Journal of Applied & Environmental Microbiology
ISSN (Print): 2373-6747 ISSN (Online): 2373-6712 Website: Editor-in-chief: Sankar Narayan Sinha
Open Access
Journal Browser
Journal of Applied & Environmental Microbiology. 2020, 8(1), 32-38
DOI: 10.12691/jaem-8-1-5
Open AccessArticle

Harnessing Biological Activities in Soil-Bacillus Strains to Promote the Discovery of New Bioactive Compounds

Ngo- Itsouhou1, 2, Etienne Nguimbi1, 3, , Rachel Moyen1 and Armel Faly Soloka Mabika1, 3

1Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences et Techniques Université Marien Ngouabi, Brazzaville, République du Congo

2Unité de Microbiologie moléculaire et Bioinformatique, Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, République du Congo

3Unité de Microbiologie moléculaire et Bioinformatique, Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, République du Congo;Institut de Recherche en Sciences Exactes et Naturelles (IRSEN), Avenue de l’Auberge Gascogne Cité scientifique (Ex. OROSTOM), Brazzaville, Republic of Congo

Pub. Date: July 10, 2020

Cite this paper:
Ngo- Itsouhou, Etienne Nguimbi, Rachel Moyen and Armel Faly Soloka Mabika. Harnessing Biological Activities in Soil-Bacillus Strains to Promote the Discovery of New Bioactive Compounds. Journal of Applied & Environmental Microbiology. 2020; 8(1):32-38. doi: 10.12691/jaem-8-1-5


The biological activities of bacteria of the genus Bacillus (MN6. MN7, MN12, MN14, MN17, and MN26) isolated from the soils at Brazzaville were explored. The capacity of six strains to produce hydrolytic enzymes, in particular: proteases, lipases, amylases, cellulases was evaluated. The antibacterial activity of each strain of Bacillus for three pathogenic strains (Escherichia coli MN40, Salmonella typhimurium MN42, Pseudomonas aeruginosa MN41) was also tested. The methods of petri dishes containing the substrate relating to the search for each hydrolytic activity were used. All Bacillus strains grow under these culture conditions. The growth of each strain of Bacillus correlated with the production of protease gave values for the optical density respectively between 0.75 ± 0.2 and 0.99 ± 0.1, while the production of proteolytic enzyme values between 11.1 ± 0.04 and 20 ± 0.11 Two strains of Bacillus MN7 and MN17 produce amylases. The MN17 strain was tested producing lipase. The MN12, MN17, MN26 strains were tested positive for the production of cellulases. The other three strains of Bacillus MN6, MN7, MN14 were tested negative for cellulase production. A principal component analysis revealed that all of the strains have antibacterial activity against at least one of the three pathogenic strains indicated. A principal component analysis indicates that the antibacterials produced by the MN6, MN14 and MN17 strains are very effective at the three pathogenic strains with greater efficacy of MN17 on E. coli MN40. MN7, MN12 and MN14 produce antibacterials which neutralize the bacteria Salmonella typhimurium MN42, Pseudomonas aeruginosa MN41. These bacteria isolated from the soil in Brazzaville, produce bioactive compounds of biotechnological interest.

exploitation biological activities Bacillus soil bioactives compounds

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit


[1]  Alexander M, 1977. Introduction to soil microbiology, 2nd edition. J. Wiley and Sons Inc. NY, 467pp.
[2]  Torsvik, V., Daae, F.L., Sandaa, R-A., Øvreås L., 1998. Novel techniques for analysing microbial diversity in natural and perturbed environments. Journal of Biotechnology 64: 53-62.
[3]  Hugenholtz, P., 2002. Exploring prokaryotic diversity in the genomic era. Genome Biol 3, reviews0003.1.
[4]  Gupta, R., Beg, Q.K., Lorenz, P., 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. ApplMicrobiolBiotechnol, 59: 15-32.
[5]  Singh, J., Batra N. and Sobti C., 2004. Purification and characterization of alkaline cellulase produced by a novel isolate, Bacillus sphaericus JS1. J. Ind. Microbiol. Biotechnol. 31, 51-56.
[6]  Berger, E., Zhang, D., Zverlov, V.V., Schwarz W.H., 2007. Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically. FEMS Microbiol Lett 268: 194-201.
[7]  Sohail, M., Siddiqi, R., Ahmad, A and Khan,S.A., 2009. Cellulase production from Aspergillus nigerMS82: Effect of temperature and pH. New Biotechnol., 25: 437-441.
[8]  Kuhad,. R.C., Gupta, R., and Singh, A.,. (2011). Microbial cellulases and their industrial applications. Enzyme Research. Volume. 10 pages.
[9]  Alani, B., Zamani, Mr., Motallebi, M., Zanghani, 2005. Purification of polygalacturonase enzyme from strain F58 Fusarium oxysporum. August 28-Sept 1 HUPO 4th Annual Word Congress, Munich.
[10]  Pandey, A., Nigam, P., Soccol, C.R, Soccol VT, Singh D and Mohan R., 2000. Advances in microbial amylases. Biotechnol. Appl. Biochem. 31, 135-152.
[11]  Kathiresen, K and Manivannan S., 2006. α-Amylase production by Penicillium fellutanum isolated from mangrove rhizosphere soil. African journal of Biotechnology; 5(10): 829-832.
[12]  Kikani, B.A and Singh, S.P., 2011. Single step purification and characterization of a thermostable and calcium independent 𝛼-amylase from Bacillus amyloliquifaciensTSWK1-1 isolated fromTulsi Shyamhot spring reservoir, Gujarat (India). International Journal of Biological Macromolecules, vol.48, no.4, pp. 676-681.
[13]  Ngo-Itsouhou., Nguimbi, E., Kayath, A. C., Ampa R., 2019. Molecular Identification, Phylogenetic Classification and Proteolytic Capacity of Cultivable Bacteria Isolated from Soils in Brazzaville, Republic of Congo Jobimb, Vol 7, No 2, 1-7.
[14]  Tadesse, G., Ephraim, E., and Ashenafi. M., 2004. Assessment of the antimicrobial activity of lactic acid bacteria isolated from Borde and Shamita, traditional Ethiopianfermented beverages, on some foodborne pathogens and effect of growth medium on the inhibitory activity. Int. J. Food Safety, 5: 13-20.
[15]  Settanni., Corsetti., 2008. Application of bacteriocins in vegetable food biopreservation. International Journal of Food Microbiology. 121(2): 123-38.
[16]  TAGG, J. R AND McGIVEN, A. R. 1971. Bacteriocin activity can be detected and assayed by a modification of the punchhole method. Applied Microbiology, May 1971, p. 943 Vol. 21, No. 5 Printed in U.S.A.
[17]  M. Rammelsberg, F.R., 1990. Antibacterial polypeptides of Lactobacillus species. Journal of Applied Microbiology. 69(2): 177-184.
[18]  Fleming, H,P., Etchells, J.L, and Costilow, R.N., 1975. Microbial inhibition by an Isolate of Pediococcus from cucumber brines. Appl. Microbiol. 30: 1040-1042.
[19]  Sabaou, N., Amir, H., et Bounaga D., 1980. Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa Volume 14, Issue 3.
[20]  Sztajer, H., Maliszewska L., Weiczorek J., 1988. Production of exogenous lipase by bacteria, Fungi and actinomycetes enzyme. Microbiol. Technol. 10: 492-7.
[21]  Schallmey, M., Singh, A., and Ward, O. P., 2004. Developments in the use of Bacillus species for industrial production. Can. 1. Microbial, 50: 1-17.
[22]  Guiraud, J.P., 2003. Microbiologie Alimentaire. Tec & Doc, Dunod. Paris. 90-292.
[23]  Fooladi, J., and Sajjadian A., 2010. Screening the thermophilic and hyperthermophilic bacterial population of three Iranian hot-springs to detect the thermostable a- amylase producing strain. Iran. J Microbiol. 2 (1): 49-53.
[24]  De ,Vos , P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A.,Schleifer K. H. and Whitman W. B., 2009. Bergey's Manual of Systematic Bacteriology, 2 ed., The Firmicute. Springer. New York. Volume Three. 63-67.
[25]  Apun, K., Jong B.C, Salleh., 2000. Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste. The Journal of General and Applied Microbiology 46(5):263-267.
[26]  Puri, S., Beg, Q., & Gupta, R., 2002. Optimization of Alkaline protéase production from Bacillus Sp. by response surface methodology Curr Microbiol, 44: 286-290.
[27]  Soloka, Mabika A, F., Moyen, R,, Nguimbi E., Ahombo, G., Ampa, R., Kayath, A, C., Vouidibio, A., Morabandza, C,J,. and Kobawila. S,C., 2017. Production, Partial Purification and Based SDS-PAGE Profilesof Caseinolytic Enzyme in two Bacillus Strains Isolated from Fermented Cassava leaves "Ntoba mbodi" in Congo Brazzaville. Journal of Pure and Applied Microbiology. Vol. 11(1), p. 77-86.
[28]  Nihan, S. and Elif D., 2011. Production of Protease by Bacillus sp. N-40 Isolated from Soil and Its Enzymatic Properties J. Biol. Environ. Sci. 5(14), 95. 103.
[29]  Adinarayana, K., Ellaiah, P., and Prasad DS.,2003. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS Pharm. Sci Tech., 4: 1-9.
[30]  Minotto, Kingchan., Malisorn1 and Kanokwan Nikhome., 2014. Isolation and screening of Actinomycetes from soil for their enzymatic and antifungal activity. 151KHON KAEN AGR. J. 42 SUPPL. 4.
[31]  Immanuel, G., E. P., 2008. Investigation of Lipase Production by Milk Isolate Serratia rubidaea. Food Technol. Biotechnol, 46: 60 65.
[32]  Smibert, R., & Krie, N., 2010. Phenotypic characterization. In Methods for General and Molecular Bacteriology. American Society for Microbiology, 617-622.
[33]  Hind, Leghlimi., 2013. Production and characterization of cellulolytic activities. Journal of biotechnology 12 (5).
[34]  Fergani et Lakhel., 2015. Activités cellulolytiques de Trichoderma longibrachiatum cultivée sur son de blé. Mémoire Master: Biotechnologie des mycètes. Constantine: Université Mentouri, 56.
[35]  Ghaferah, H., Amani, S., Monerah, R., 2018. Anticandidal activity of the extract and compounds isolated from Cyperus conglomertus Rottb. Saudi Pharm J 26:891-5.
[36]  Al-Hazmi, GH., Awaad AS, Alothman MR, et al, 2018. Antican-didal activity of the extract and compounds isolated fromCype-rus conglomertus Rottb. Saudi Pharm J. 26: 891-5.
[37]  Gayathri, A., Madhanraj, P; and Panneerse lvam A., 2011. Diversity, Antibacterial Activity And Molecular Characterization of Actinomycetes Isolated From Salt Pan Region of Kodiakarai, Nagapattinam, DT. Asian J. Pharm. Tech. Vol: 1. N° 3. Pp: 79-81.
[38]  Khalil A, Mohamad., A, Hassan ,H, Hani, O., 2015. Antibacterial activity of four herbal extracts against methicillin resistant Bacterial strains isolated from patients in Almadinah hospitals, Saudi Arabia. IJASR International Journal of Academic Scientific Research ISSN: Volume 3, Issue 1, PP 34-40.
[39]  Mahadik, ND., Puntam bekar US, Bastawde KB., 2002. Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochem 38: 715-721.