Environmental Hazard Evaluation of Fecal Indicator Bacteria and Hepatitis A Virus in River Owena

Adewale Oluwasogo Olalemi^1,

¹Department of Microbiology, Federal University of Technology, Akure, Nigeriay

Cite this paper:
Adewale Oluwasogo Olalemi. Environmental Hazard Evaluation of Fecal Indicator Bacteria and Hepatitis A Virus in River Owena. Journal of Applied & Environmental Microbiology. 2019; 7(1):3-8. doi: 10.12691/jaem-7-1-2

Abstract

This study was carried out to determine the suitability of fecal indicator bacteria to predict human health risks associated with hepatitis A virus in a river in Nigeria, representing a regional situation in comparison with other global aspects. Water samples were collected from River Owena weekly over a period of twelve weeks i.e., July-September, 2017. The concentration of Escherichia coli, fecal coliforms, Salmonella and Shigella were determined by standard microbiological method. The concentration of hepatitis A virus was determined using standard molecular detection technique. Physicochemical properties of the water samples were determined using standard methods. Results showed that the concentration of Escherichia coli in the water samples ranged from 4.11 to 4.35 log₁₀ CFU 100 ml^-1 whereas those of fecal coliforms ranged from 4.23 to 4.51 log₁₀ CFU 100 ml^-1. Whilst the concentrations of the bacterial indicators correlated positively, there was no significant relationship between the concentration of hepatitis A virus and those of the bacterial indicators in the water samples. The findings from this study suggest that the sanitary quality of surface waters based on bacterial indicators may be inadequate in protecting human health from risks associated with hepatitis A virus.

Keywords:
fecal indicator bacteria hepatitis a virus human health risk assessment surface waters

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References:

[1]	Rzeżutka, A. and Cook, N. Viruses: Hepatitis A Virus. Encyclopedia of Food Safety. (Motarjemi, Y, ed.) Waltham, M.A, Academic Press, Elsevier, 2014, 198-204.
[2]	WHO. Global hepatitis report. World Health Organization, Geneva, Switzerland, 2017, 68.
[3]	Coudray-Meunier, C., Fraisse, A., Martin-Latil, S., Guillier, L., Delannoy, S., Fach, P. and Perelle, S. “A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples,” International Journal of Food Microbiology, 201. 17-26. 2015.
[4]	Prevost, B., Lucas, F.S., Goncalves, A., Richard, F., Moulin, L., & Wurtzer, S. “Large scale survey of enteric viruses in river and waste water underlines the health status of the local population,” Environment International, 79. 42-50. 2015.
[5]	Olalemi, A. Bacteriophages as surrogates of viral pathogens: a novel tool for the shellfisheries industry. PhD Thesis, Environment and Public Health Research Group, University of Brighton, Brighton, UK. 2015, 232.
[6]	Chigor, V.N. and Okoh, A.I. “Quantitative RT-PCR detection of hepatitis A virus, rotaviruses and enteroviruses in the Buffalo River and source water dams in the Eastern Cape Province of South Africa,” International Journal of Environmental Research and Public Health, 9, 4017-4032. 2012.
[7]	Zhu, H., Yuan, F., Liu, R., Xie, F., Huang, L., Liu, X., Jiang, X., Wang, J., Xu, Q., Shen, Z., Liu, D., Zhang, R. & Lu, Y. “Monitoring of Poyang lake water for sewage contamination using human enteric viruses as an indicator,” Virology Journal, 15(3), 1-9. Jan., 2018.
[8]	Alidjinou, E.K., Sane, F., Firquet, S., Lobert, P.E. and Hober, D. “Resistance of enteric viruses on formites,” Intervirology, 9. 2017.
[9]	Sunger, N., Hamilton, K.A., Morgan, P.M. and Haas, C.N. “Comparison of pathogen-derived ‘total risk’ with indicator-based correlations for recreational (swimming) exposure,” Environmental Science and Pollution Research, 1-11. Apr., 2018.
[10]	De Giglio, O., Caggiano, G., Bagordo, F., Barbuti, G., Brigida, S., Lugoli, F., Grassi, T., La Rosa, G., Lucentini, L., Uricchio, V.F., De Donno, A. and Montagna, M.T. “Enteric viruses and fecal bacteria indicators to assess groundwater quality and suitability for irrigation,” International Journal of Environmental Research and Public Health, 14(6), 558-571. May, 2017.
[11]	Fernández-Molina, M.C., Álvarez, A. and Espigares, M. “Presence of hepatitis A virus in water and its relationship with indicators of fecal contamination,” Water, Air and Soil Pollution, 159(1), 197-208. Nov., 2004.
[12]	Gamazo, P., Victoria, M., Schijven, J.F., Alvareda, E., Tort, L.F.L., Ramos, J., Burutaran, L., Olivera, M., Lizasoain, A., Sapriza, G., Castells, M. and Colina, R. “Evaluation of bacterial contamination as an indicator of viral contamination in a sedimentary aquifer in Uruguay,” Food and Environmental Virology, 1-11. Mar., 2018.
[13]	Anon. Standard methods for the examination of water and wastewater (22nd ed.) APHA/AWWA/WEF: Washington DC, USA. 2012.
[14]	Anon. ISO 9308 – 1. Water Quality. Detection and Enumeration of Escherichia coli and Coliform Bacteria – Part 1: Membrane Filtration Method for waters with low bacterial background flora. Geneva, Switzerland: International Organisation for Standardisation. 2014.
[15]	Harwood, V.J., Boehm, A.B., Sassoubre, L.M., Vijayavel, K., Stewart, J.R., Fong, T.T., Caprais, M., Converse, R.R, Diston, D., Ebdon, J., Fuhrman, J.A., Gourmelon, M., Gentry-Shields, J., Griffith, J.F., Kashian, D.R., Noble, R.T., Taylor, H., Wicki, M. “Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study,” Water Research, 47(18), 6929-6943. 2013.
[16]	Mendez, J., Audicana, A., Isern, A., Llaneza, J., Moreno, B., Tarancon, M.L., Jofre, J., Lucena, F. “Standardised evaluation of the performance of a simple membrane filtration-elution method to concentrate bacteriophages from drinking water,” Journal of Virological Methods, 117(1), 19-25. 2004.
[17]	Olalemi, A., Purnell, S., Caplin, J., Ebdon, J. and Taylor, H. “The application of phage‐based fecal pollution markers to predict the concentration of adenoviruses in mussels (Mytilus edulis) and their overlying waters,” Journal of Applied Microbiology, 121(4), 1152-1162. Aug., 2016.
[18]	Kavka, G.G., Kaimir, G.D. and Farnleitner, A.H. “Microbiological water quality of the River Danube (Km 2581 – Km 15): longitudinal variation of pollution as determined by standard parameters,” In: Proceedings 36th International Conference of IAD. Austrian Committee for Danube Research/IAD, Vienna, 415-421. 2006.
[19]	Nnane, D.E., Ebdon, J.E. and Taylor, H.D. “Integrated analysis of water quality parameters for cost-effective fecal pollution management in river catchments,” Water Research, 45, 2235-2246. 2011.
[20]	Council of European Union (CEU) “Directive 2006/7/EC of the European Parliament and of the council of 15 February 2006 concerning the management of bathing water quality and repealing Directive 76/160/EEC,” Journal of the European Union, 2006.
[21]	Wilkes, G., Brassard, J., Edge, T.A., Gannon, V., Jokinen, C.C., Jones, T.H., Neumann, N., Pintar, K.D.M., Ruecker, N., Schmidt, P.J., Sunohara, M., Topp, E. and Lapen, D.R. “Bacteria, viruses, and parasites in an intermittent stream protected from and exposed to pasturing cattle: Prevalence, densities, and quantitative microbial risk assessment,” Water Research, 47(16), 6244-6257. 2013.
[22]	Kostyla, C., Bain, R., Cronk, R., Bartram, J. “Seasonal variation of fecal contamination in drinking water sources in developing countries: A systemic review,” Science of the Total Environment, 514, 333-343. 2015.
[23]	Sewlikar, S. and D’Souza, D.H. “Survival of hepatitis A virus and Aichi virus in cranberry-based juices at refrigeration (4°C),” Food Microbiology, 62, 251-255. 2017.
[24]	Peterson, D.A., Wolfe, L.G., Larkin, E.P. and Deinhardt, F.W. “Thermal treatment and infectivity of hepatitis A virus in human feces,” Journal of Medical Virology, 2, 201-206. 2008.
[25]	Ashbolt, N.J. Grabow, W.O. and Snozzi, M. Indicators of microbial water quality. In Fewtrell, L. and Bartram, J. (ed.), Water Quality: Guidelines, Standards and Health. IWA Publishing, London, United Kingdom, 2001, 289-316.
[26]	Sinclair, R.G. Rose, J.B. Hashsham, S.A. Gerba, C.P. and Haas, C.N. “Criteria for selection of surrogates used to study the fate and control of pathogens in the environment,” Applied and Environmental Microbiology, 78, 1969-1977. 2012.
[27]	Derne B., Weinstein P., Lau C. Wetlands as sites of exposure to waterborne infectious diseases. In: Finlayson C., Horwitz P., Weinstein P. (eds) Wetlands and human health. Wetlands: Ecology, Conservation and Management, 5. Springer, Dordrecht, 2015, 45-74.
[28]	Van der Poel, W. and Rzezutka, A. Hepatitis A. Global Water Pathogens Project. In: J. S. Meschke, and R. Girones (eds) Part 3 Viruses. Michigan State University, E. Lansing, MI, UNESCO, 2017.