Assessment of Haemoglobin Variants in Malaria Infected Individuals Using Haematological Parameters

Esan A.J^1,

¹Haematology Department, Federal Teaching Hospital, Ido-Ekiti, Ekiti State Nigeria

Cite this paper:
Esan A.J. Assessment of Haemoglobin Variants in Malaria Infected Individuals Using Haematological Parameters. International Journal of Hematological Disorders. 2015; 2(1):4-9. doi: 10.12691/ijhd-2-1-2

Abstract

The aim of this study was to determine the effect of haemoglobin variants in Plasmodium falciparum infected individuals using haematological parameters. The study was conducted at Federal Teaching Hospital, Ido-Ekiti Nigeria. Two hundred and two blood samples were collected twice from each malaria infected individuals; grouped as pre-treatment and post anti-malaria drug treatment; 4ml of blood sample was collected and dispensed into K₂EDTA vaccutainer bottles for haemoglobin electrophoresis using cellulose acetate electrophoresis as described by Dacie and Lewis, haemoglobin concentration, packed cell volume and red cell indices were analysed using haematology analyser, thick blood film was made and stained with Giemsa's staining technique for malaria parasite detection as described by Monica Cheesbrough. Data obtained was analysed using SPSS version 16. The result of this present study showed that, the mean±SD of red blood cell, haemoglobin concentration, packed cell volume, red cell indices and red cell distribution width of HbAA in pre and post anti-malaria drug treatment were significantly (P<0.05) lower compared to HbAS and HbAC in pre and post anti-malaria drug treatment. This present study showed that, HbAS and HbAC suffer less malaria attack with lower incidence of clinical malaria compared to HbAA.

Keywords:
malaria parasite haemoglobin variants and haematological parameters

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References:

[1]	UNICEF. Rolling back malaria goals. A United Nation Children’s Fund document. (2000); 16pp.
[2]	Alaribe AAA, Ejekie GC, Ezedinachi ENU. The ecology of Bain BJ (1996). Ethnic and sex differences in the total and differential white cell count and platelet count. J Clin Pathol. (2006); 49: 664-666.
[3]	Tiffert T, Lew VL, Ginsburg H, et al. The hydration state of human red blood cells and their susceptibility to invasion by Plasmodium falciparum. Blood; (2005)105:4853.
[4]	Ayi K, Turrini F, Piga A, Arese P. Enhanced phagocytosis of ring-parasitized mutant erythrocytes: a common mechanism that may explain protection against falciparum malaria in sickle trait and beta-thalassemia trait. Blood; (2004)104: 3364-3371.
[5]	Rihet P, Flori L, Tall F, Traore AS, Fumoux F: Hemoglobin C is associated with reduced Plasmodium falciparum parasitemia and low risk of mild malaria attack. Hum Mol Genet, (2004) 13: 1-6.
[6]	Kreuels B, Kreuzberg C, Kobbe R, Ayim-Akonor M, Apiah-Thompson P, Thompson B, Ehmen C, Adjei S, Langefeld I, Adjei O, May J: Differing effects of HbS and HbC traits on uncomplicated falciparum malaria, anemia, and child growth. Blood, (2010) 115: 4551-4558.
[7]	Williams TN, Mwangi TW, Wambua S, Alexander ND, Kortok M, Snow RW, Marsh K: Sickle cell trait and the risk of Plasmodium falciparum malaria and other childhood diseases. J Infect Dis, (2005) 192: 178-186.
[8]	Williams TN, Mwangi TW, Roberts DJ, Alexander ND, Weatherall DJ, Wambua S, Kortok M, Snow RW, Marsh K: An immune basis for malaria protection by the sickle cell trait. PLoS Med, (2005)2:e128.
[9]	Verra F, Bancone G, Avellino P, Blot I, Simpore J, Modiano D: Haemoglobin C and S in natural selection against Plasmodium falciparum malaria: a plethora or a single shared adaptive mechanism? Parassitologia, (2007); 49: 209-213.
[10]	Le Hesran JY, Personne I, Personne P, Fievet N, Dubois B, Beyeme M, Boudin C, Cot M, Deloron P: Longitudinal study of Plasmodium falciparum infection and immune responses in infants with or without the sickle cell trait. Int J Epidemiol, (1999) 28:793-798.
[11]	Williams TN: How do hemoglobins S and C result in malaria protection? J Infect Dis, (2011) 204: 1651-1653.
[12]	Williams TN, Obaro SK: Sickle cell disease and malaria morbidity: a tale with two tails. Trends Parasitol, (2011) 27:315-320.
[13]	Kennedy JR: Malaria: a vaccine concept based on sickle haemoglobin's augmentation of an innate autoimmune process to band 3. Int J Parasitol, (2001); 31; 1275-1277.
[14]	Roberts DJ, Williams TN : Haemoglobinopathies and resistance to malaria. Redox Rep, (2003); 8: 304-310.
[15]	Ferreira A, Marguti I, Bechmann I, Jeney V, Chora A, Palha NR, Rebelo S, Henri A, Beuzard Y, Soares MP: Sickle hemoglobin confers tolerance to Plasmodium infection. Cell, (2011); 145:398-409.
[16]	Monica Cheesbrough. Discrete Laboratory Practice in Tropical Countries Part 1, Cambridge Second Editions. Published by Press Syndicate of the University of Cambridge, (2005); chp. 5, page 247-258.
[17]	Uzoegwu P.N and Onwurah A.E. Prevalence of heamoglobinopathy and malaria disease in the population of old Aguata Division, Anambra State, Nigeria. Biokemistri, (2003)15: 57-66.
[18]	Francis M. AWAH and Pete N. UZOEGWU. Influence of sickle heterozygous status and glucose-6-phosphate dehydrogenase deficiency on the clinico-haematolgoical profile of Plasmodium falciparum-infected children Biokemistri (2006) 18(2):89-97.
[19]	Edith C Bougouma, Alfred B Tiono, Alphonse Ouédraogo, Issiaka Soulama, Amidou Diarra, Jean-Baptiste Yaro, Espérance Ouédraogo, Souleymane Sanon, Amadou T Konaté, Issa Nébié,. Haemoglobin variants and Plasmodium falciparum malaria in children under five years of age living in a high and seasonal malaria transmission area of Burkina Faso; Malaria Journal (2012)11: 154.
[20]	Zuk M, McKean KA. Sex differences in parasite infections: patterns and processes. Inter J Parasitol; (1996) 26: 1009-23.
[21]	Esan Ayodele. J. Severity and Prevalence of Malaria Infection and Effect of Anti-Malaria Drugs on Gender Differences Using Some Haematological Parameters * International Journal of Hematological Disorders, 2014, Vol. 1, No. 1, 1-7.
[22]	ESAN AYODELE J. Effect of Anti-malaria Drugs on Some Blood Cell Lines Parameters in Adult Individuals Infected with Acute Uncomplicated Plasmodium falciparum Malaria. International Journal of Hematological Disorders, 2014, Vol. 1, No. 1, 12-21.