Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07

Maryam L. Riskuwa-Shehu^1, and Udeme J.J. Ijah²

¹Department of Microbiology, Usmanu Danfodiyo University Sokoto, Sokoto state, Nigeria

²Department of Microbiology, Federal University of Technology, Minna, Niger state, Nigeria

Cite this paper:
Maryam L. Riskuwa-Shehu and Udeme J.J. Ijah. Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07. International Journal of Environmental Bioremediation & Biodegradation. 2016; 4(1):8-12. doi: 10.12691/ijebb-4-1-2

Abstract

Oil degrading bacteria were isolated from polluted soil from Eremu, Delta State, Nigeria and identified as species of Bacillus, Pseudomonas, Alcaligenes and Micrococcus. The bacteria were able to utilize Ubefan (Nigerian) light crude oil as a source of carbon and energy. Two isolates, Bacillus megaterium UL05 and Pseudomonas aeruginosa UL07, which had very high ability in utilizing the crude oil, were used as mixed culture for bioremediation studies. Results showed that addition of the mixed bacterial culture to oil polluted soil caused a decreased in pH of the soil and a reduction in the peaks of the soil components as revealed by the GLC analysis. The hydrocarbon components (C₅ – C₁₂) were extensively degraded in all treatments. In oil polluted soil inoculated with bacteria, C14 – C38 were highly degraded as compared to the control non-bioaugmented soil. These results suggest that Bacillus megaterium UL05 and Pseudomonas aeruginosa UL07 can be useful in treating oil spills in tropical soil.

Keywords:
bioremediation enhanced removal bacterial crude oil mixed culture soil

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