Article citationsMore >>

Miller, H. “Biostimulation as a form of bioremediation of soil pollutants,” Basic Biotechnol. 6, 7-12, 2010.

has been cited by the following article:

Article

Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07

1Department of Microbiology, Usmanu Danfodiyo University Sokoto, Sokoto state, Nigeria

2Department of Microbiology, Federal University of Technology, Minna, Niger state, Nigeria


International Journal of Environmental Bioremediation & Biodegradation. 2016, Vol. 4 No. 1, 8-12
DOI: 10.12691/ijebb-4-1-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Maryam L. Riskuwa-Shehu, Udeme J.J. Ijah. Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07. International Journal of Environmental Bioremediation & Biodegradation. 2016; 4(1):8-12. doi: 10.12691/ijebb-4-1-2.

Correspondence to: Maryam  L. Riskuwa-Shehu, Department of Microbiology, Usmanu Danfodiyo University Sokoto, Sokoto state, Nigeria. Email: mlriskuwa@hotmail.com

Abstract

Oil degrading bacteria were isolated from polluted soil from Eremu, Delta State, Nigeria and identified as species of Bacillus, Pseudomonas, Alcaligenes and Micrococcus. The bacteria were able to utilize Ubefan (Nigerian) light crude oil as a source of carbon and energy. Two isolates, Bacillus megaterium UL05 and Pseudomonas aeruginosa UL07, which had very high ability in utilizing the crude oil, were used as mixed culture for bioremediation studies. Results showed that addition of the mixed bacterial culture to oil polluted soil caused a decreased in pH of the soil and a reduction in the peaks of the soil components as revealed by the GLC analysis. The hydrocarbon components (C5 – C12) were extensively degraded in all treatments. In oil polluted soil inoculated with bacteria, C14 – C38 were highly degraded as compared to the control non-bioaugmented soil. These results suggest that Bacillus megaterium UL05 and Pseudomonas aeruginosa UL07 can be useful in treating oil spills in tropical soil.

Keywords