International Journal of Environmental Bioremediation & Biodegradation
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International Journal of Environmental Bioremediation & Biodegradation. 2014, 2(4), 160-166
DOI: 10.12691/ijebb-2-4-3
Open AccessArticle

Exploited Application of Lactobacillus in Microbial Degradation and Decolorization of Acid Orange

Maulin P Shah1,

1Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology, Enviro Technology Limited, GIDC, Ankleshwar, Gujarat, India

Pub. Date: July 03, 2014

Cite this paper:
Maulin P Shah. Exploited Application of Lactobacillus in Microbial Degradation and Decolorization of Acid Orange. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(4):160-166. doi: 10.12691/ijebb-2-4-3

Abstract

A bacterium identified as Lactobacillus was isolated from acclimated sludge from a dyeing wastewater treatment plant. This strain rapidly decolorized Reactive orange (RO) at 0.1% (w/v) concentration of both sucrose and peptone supplemented in Mineral Salt Medium (MSM) under static conditions at a temperature of 30°C with in 24 h with an initial dye concentration of 100 mg/L. The organism exhibited a remarkable color removal capability (95%) even at a high concentration of 1000 mg/L (RO16) dye within 24 h. The biodegradation products were analyzed by, FTIR spectroscopy and LC-MS analysis. The LC-MS analysis indicated the presence of 1-amino- 1-napthalene sulphonic acid in degraded product of the dye.

Keywords:
Lactobacillus biodegradation Reactive orange decolorization

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References:

[1]  Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. International Biodeterioration & Biodegradation 59: 73-84.
 
[2]  Vijaykumar MH, Vaishampayan PA, Shouche YS, Karegoudar TB (2007) Decolourization of naphthalene-containing sulfonated azo dyes by Kerstersia sp strain VKY1. Enzyme Microbial Technology 40: 204-211.
 
[3]  Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastgheib SM (2007) Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresources Technology 98: 2082-2088.
 
[4]  Ozturk A, Abdullah M (2006) Toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions. Science of the Total Environment 358: 137-142.
 
[5]  Moller P, Wallin H (2000) Genotoxic hazards of azo pigments and other colorants related to 1-phenylazo-2-hydroxynaphthalene. Mutation Research 462: 13-30.
 
[6]  Okazaki S, Nagasawa S, Goto M, Furusaki S, Wariishi H, et al. (2002) Decolorization of azo and anthraquinone dyes in hydrophobic organic media using microperoxidase-11 entrapped in reversed micelles. Biochemical Engineering Journal 12: 237-241
 
[7]  Van der Zee FP, Santiago V (2005) Combined anaerobic-aerobic treatment of azo dyes-a short review of bioreactor studies. Water Research 39: 1425-1440.
 
[8]  Isik M, Sponza DT (2004) Decolorization of azo dyes under batch anaerobic and sequential anaerobic/aerobic conditions. Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering 39: 1107-1127.
 
[9]  Xu M, Guo J, Sun G (2007) Biodegradation of textile azo dye by Shewanella decolorationisS12 under microaerophilic conditions. Applied Microbiology and Biotechnology 76: 719-726.
 
[10]  Stolz A (2001) Basic and applied aspects in the microbial degradation of azo dyes. Applied Microbiology and Biotechnology 56: 69-80.
 
[11]  Seesuriyachan P, Takenaka S, Kuntiya A, Srikarnjana S, Shuichiro S, et al. (2006) Metabolism of azo dyes by Lactobacillus casei TISTR 1500 and effects of various factors on decolorization. Water research 41: 985-992.
 
[12]  Grifoni A, Bazzicalupo M, Di Serio C, Fancelli S, Fani R (1995) Identification of Azospirillum strains by restriction fragment length polymorphism of the 16S rDNA and of the histidine operon. FEMS Microbiol Lett 127: 85-91.
 
[13]  Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245.
 
[14]  Dave SR, Dave RH (2009) Isolation and characterization of Bacillus thuringiensis for acid red 119 dye decolourisation. Bioresour Technol 100: 249-253.
 
[15]  Lee YH, Matthews RD, Pavlostathis SG (2005) Biological decolorization of reactive anthraquinone and phthalocyanine dyes under various oxidation-reduction conditions. Water Environ Res 78: 156-169.
 
[16]  Hsueh CC, Chen BY (2007) Comparative study on reaction selectivity of azo dye decolorization by Pseudomonas luteola. J Hazard Mater 141: 842-849.
 
[17]  Hu TL (1994) Decolourization of reactive azo dyes by transformation with Pseudomonas luteola. Bioresource Technol 49: 47-51.
 
[18]  Chang JS, Kuo TS (2000) Kinetics of bacterial decolorization of azodye with Escherichia coli NO3. Bioresour Technol 75: 107-111.
 
[19]  Pearce CI, Lloyd JR, Guthrie JT (2003) The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes Pigm 58: 179-196.
 
[20]  Oei BC, Ibrahim S, Wang S, Ang HM (2009) Surfactants modified barley straw for removal of acid and reactive dyes from aqueous solution. Bioresour Technol 100: 4292-4295.