Eco-Friendly Treatment of Acid Red by an Application of Pseudomonas spp

Maulin P Shah^1,

¹Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Plot GIDC, Ankleshwar Gujarat, India

Cite this paper:
Maulin P Shah. Eco-Friendly Treatment of Acid Red by an Application of Pseudomonas spp. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(2):62-68. doi: 10.12691/ijebb-2-2-3

Abstract

Acid red decolorizing bacteria was isolated and identified as Pseudomonas spp. The effect of operation parameters such as medium composition, pH, temperature, dye concentration on the decolorization of acid red was studied and the products of degradation were analyzed and confi rmed using LC–MS analysis. The reductive cleavage of azo bond was catalyzed by azoreductase, the key enzyme for the azo dye degradation. The catalytic reduction of acid red 37 by purifi ed azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5- [(aminoxy) sulfonyl] phenyl} ethanol and 7,8- diamino-3 [(aminoxy) sulfonyl] naphthalene-1-ol.

Keywords:
acid red pseudomonas LC-MS azo reductase

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Campos R, Kandelbauer A, Robra KH, Cavaco-Paulo A, Gübitz GM (2001) Indigo degradation with purified laccases from Trametes hirsuta and Sclerotim rolfsii. J Biotechnol 89: 131-139.
[2]	Zollinger H (1987) Color Chemistry Syntheses: Properties and Application of Organic Dyes and Pigments. (2nd edn), VCH Publications, New York.
[3]	Devi M, Kaushik BD (2005) Decolorization of textile dyes and dye effluent by Aspergillus Spp. Indian J Microbiol 45: 41-44.
[4]	Shaul GM, Holdsworth TJ, Dempsey CR, Dostal KA (1991) Fate of water soluble azo dyes in the activated sludge process. Chemosphere 22: 107-119.
[5]	O’Neill C, Hawkes FR, Hawkes DL, Lourenco ND, Pinheiro HM, et al. (1999) Colour in textile effluents-sources, measurement, discharge consents and simulation: a review. Journal of Chemical Technology and Biotechnology 74: 1009-1018.
[6]	McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, et al. (2001) Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol 56: 81-87.
[7]	Chung K, Stevens Jr SE (1993) Decolourization of azo dyes by environmental microorganisms and helminths. Environ Toxicol Chem 12: 2121-2132.
[8]	Baldrian P, Gabriel J (2003) Lignocellulose degradation by Pleurotus ostreatus in the presence of cadmium. FEMS Microbiol Lett 220: 235-240.
[9]	Ponraj M, Gokila K, Zambare V (2011) Bacterial decolorization of textile dye- Orange 3R. International Journal of Advanced Biotechnology and Research 2: 168-177.
[10]	Grifoni A, Bazzicalupo M, Di Serio C, Fancelli S, Fani R (1995) Identifi cation of Azospirillum strains by restriction fragment length polymorphism of the 16S rDNA and of the histidine operon. FEMS Microbiol Lett 127: 85-91.
[11]	Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textiledye-containing effl uents: a review. Bioresour Technol 58: 217-227
[12]	Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245.
[13]	Moutaouakkil A, Zeroual Y, Zohra Dzayri F, Talbi M, Lee K, et al. (2003) Purifi cation and partial characterization of azoreductase from Enterobacter agglomerans. Arch Biochem Biophys 413: 139-146.
[14]	Maier J, Kandelbauer A, Erlacher A, Cavaco-Paulo A, Gübitz GM (2004) A new alkali-thermostable azoreductase from Bacillus sp. strain SF. Appl Environ Microbiol 70: 837-844.
[15]	Wong PK, Yuen PY (1996) Decolorization and Biodegradation of Methyl Red by K. Pneumoniae RS-13. Water Research 30: 1736-1744.
[16]	Mali PL, Mahajan MM, Patil DP, Kulkarni MV (2000) Biodecolorization of members of triphenylmethanes and azo groups of dyes. J Sci Ind Res 59: 221-224.
[17]	Lee YH, Matthews RD, Pavlostathis SG (2005) Biological decolorization of reactive anthraquinone and phthalocyanine dyes under various oxidationreduction conditions. Water Environ Res 78: 156-169.
[18]	Hsueh CC, Chen BY (2007) Comparative study on reaction selectivity of azo dye decolorization by Pseudomonas luteola. J Hazard Mater 141: 842-849.
[19]	Tan NCG, Borger A, Slenders P, Svitelskaya A, Lettinga G, et al. (2000) Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor. Water Sci Technol 42: 337-344.