Monitoring of Cellulase and Biosurfactant Production in Bacteria Isolated from the Likouala Peat Bog (Republic of Congo)

Gatse Elgie Viennechie^{1, 2}, Mboukou Kimbatsa Irène Marie Cecile^{2, 3}, Onyankouang Isaac Samuel², Morabandza Cyr Jonas^{1, 2,} , Goma Tchimbakala Joseph² and Nguimbi Etienne^{1, 2}

¹Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville

²Laboratory of Cellular and Molecular Biology, FST-UMNG, B.P 69 Brazzaville

³Laboratory of Geomatics and Applied Tropical Ecology

Cite this paper:
Gatse Elgie Viennechie, Mboukou Kimbatsa Irène Marie Cecile, Onyankouang Isaac Samuel, Morabandza Cyr Jonas, Goma Tchimbakala Joseph and Nguimbi Etienne. Monitoring of Cellulase and Biosurfactant Production in Bacteria Isolated from the Likouala Peat Bog (Republic of Congo). International Journal of Environmental Bioremediation & Biodegradation. 2023; 11(1):1-8. doi: 10.12691/ijebb-11-1-1

Abstract

The production of cellulases and biosurfactants of isolated bacteria from the Likouala peat bog (Congo-Brazzaville) was studied. Growth and production of cellulases and biosurfactants were monitored by conventional techniques on Mossel and TSB media enriched with petroleum or vegetable oil. Correlation coefficients between cellulases and biosurfactants production were calculated with Microsoft Excel 2016 software. The results showed optical densities from 0.634 to 1; diameters of translucent halos from 1.4 to 5.8cm and emulsification indices of isolates from 0 to 80%. The production time of cellulases and biosurfactants was 24 to 96 h with an optimum at 72h for biosurfactants depending on the isolate. The isolate correlation index were: r=0.74 and r=0.53 for RE 20; r=0.87 and r=0.60 for RE 21 and r=0.96 and 0.91 for RE 24 on Mossel; r=0.77 and r=0.61 for BV1; r=0.96 and r=0.95 for BV2; r=0.95 and r=0.52 for BV3 and r=0.92 and r=0.83 for BV4 on TSB enriched with petroleum; r=0.95 and r=0.86 for BV6; 0.54 and 0.57 for BV7 and r=0.84 and r=0.95 for BV9 on TSB enriched with vegetable oil respectively for the production of cellulases and biosurfactants. The Likouala peat bog area is rich in hydrocarbonoclast bacteria that produce cellulases and biosurfactants from peat.

Keywords:
biosurfactant cellulase correlation bacteria bog

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

References:

[1]	Dargie, G.C., Lewis, S.L., Lawson, I.T., Mitchard, E.T.A., Page, S.E., Bocko, Y.E., Ifo, S. A. 2017. Age, extent and carbon storage of the central Congo Basin peatland complex. Nature 542(7639) :86–90.
[2]	Dargie, G.C., Lawson, I.T., Rayden, T.J., Miles, L., Michard, E.T.A., Page, S.E., Bocko, Y.E., Ifo, S.A., Lewis, S.L. 2018. Les tourbières du bassin du Congo : les menaces et les mesures de conservation prioritaires le complexe à tourbières dans le centre du bassin du Congo : âge, étendue et bilan de carbone. Nature
[3]	Bahouli, M. et Zidalmal, B. 2020. Valorisation des déchets lignocellulosique par fermentation en milieu solide pour la production de cellulases microbiennes. Mémoire de Master en Biochimie, Université des freres Mentouri, constantine 1. Republique populaire d’Algerie, 68p.
[4]	Khelil, O. 2017. Production de cellulase et d’enzymes associées par des souches de Bacillus sp : rôle des prétraitements et l’effet des polyphénols, des flavonoïdes et des biosurfactant. Thèse en vue de l’obtention du diplôme de Doctorat. Université des Sciences et de la technologie d’Oran ; MOHAMED BOUDIAF. 202p
[5]	Touijer, H., Benchemsi, N., Mohamed, E., Janati Idrissi, A., Chaouni, B., Bekkari, H. 2019. Thermostable Cellulases fromb the yeast Trichosporon sp. Enzyme Research, 1-6.
[6]	Martinho, V., Lima Dos Santos, L.M., Barros, A.C., Ferrari, V.B., Passarini, M.R.Z., Santos, L.A., De Souza, S.F.L., Lacava, P.T. and De Vasconcellos, S.P. 2019. Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil AMB Expr 9:130 ; 1-8.
[7]	Zheng T., Jiang J., Yao J.. (2021), Surfactant-promoted hydrolysis of lignocellulose for ethanol production Fuel Process. Tech., 213 Article 106660.
[8]	Santos, D., Rufino, R., Luna, J., Santos, V., Sarubbo, L. 2016. Biosurfactants : Multifunctional Biomolecules of the 21st Century. International Journal of Molecular Sciences, 17(3), 401.
[9]	Magdalena P-P, Grazyna AP, Zofia P-S, Cameotra SS. 2011: Environmental applications of biosurfactants: Recent advances. Int J Mol Sci. 12: 633-654. 10.3390/ijms12010633.
[10]	Mulligan CN: Environmental applications for biosurfactants. Environ Pollution. 2005, 133: 183-198. 10.1016/j.envpol.2004.06.009.
[11]	Morabandza C. J., Gatsé E. V., Mboukou K.I. M. C., Onyankouang I. S., Ifo S. A., Nguimbi E. 2022. Characterization of Isolated Bacteria from Soils in the Likouala Peat Bog Area (Republic of Congo)” American Journal of Microbiological Research, vol. 10, no. 2 (2022): 59-70.
[12]	Hentati, D. 2018. Isolement et caractérisation des bactéries marine hydrocarbonoclastes, production des biosurfactants et étude de la biodiversité microbienne au sein de trois ports de Sfax, Tunisie. Thèse pour obtenir le grade de docteur de l’université de Montpellier; École doctorale Biodiversité, Agriculture, Alimentation, Environnement, Terre, Eau ; Laboratoire de Biotechnologie de l’Environnement, Institut National de la Recherche Agronomique (LBE-INRA), France.274p.
[13]	Ngô, I., Nguimbi, E., Kayath, C., Ampa, R. 2019. Molecular Identification and Phylogenetic classification and Proteolytic capacity of cultivable Bacteria Isolated from soils in Brazzaville, Republic of Congo. Journal of Biochemestry,Microbiology and Biotechnology.
[14]	Priscillia Renée Nguimbi-Tsati, Christian Aimé Kayath, Nicaise Saturnin Mokemiabeka, Tarcis Baloki Ngoulou, Thantique Moutali Lingouangou, Yannich Okouakoua, and Gabriel Ahombo, “Production of Biosurfactants and Hydrolytic Enzymes by Bacillus-Genus Bacteria Isolated from Mbala Pinda, Traditional Food, Republic of Congo.” ajmr, vol. 10, no. 3 (2022): 76-83.
[15]	Nguimbi etienne., Ahombo Gabriel, Moyen r., Ampa r., Vouidibio a., Ontsira e. n., Kobawila s. c., Louembe d. (2014). optimization of growtheures, fibrinolytic enzyme production and pcr amplification of encoding fibrinolytic enzyme gene in bacillus amyloliquefaciens isolated from ntoba mbodi at brazzaville. international journal of science and research (ijsr), volume 3 issue 11, 2319-7064
[16]	Soloka M.F., Moyen R., Nguimbi E., Ahombo G., Ampa R., Kayath A.C., Vouidibio A., Morabandza C.J., Kobawila S.C., (2017). Production, Partial Purification and Based SDS-PAGE Profiles of Caseinolytic Enzyme in two Bacillus Strains Isolated from Fermented Cassava leaves “Ntobambodi” in Congo Brazzaville. Journal of Pure and Applied Microbiology. March. Flight. 11 (1), p. 77-86.
[17]	Kannan n., Aravindan r. and Yirutheuresagiri t. (2011). effect of culture conditions and kinetic studies on extracellular tannase production by lactobacillus plantarummtcc1407.). indian journal of biotecheuresnology, vol 10, pp321-328.