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Ngô, I., Nguimbi, E., Kayath, C., Ampa, R. 2019. Molecular Identification and Phylogenetic classification and Proteolytic capacity of cultivable Bacteria Isolated from soils in Brazzaville, Republic of Congo. Journal of Biochemestry,Microbiology and Biotechnology.

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Article

Monitoring of Cellulase and Biosurfactant Production in Bacteria Isolated from the Likouala Peat Bog (Republic of Congo)

1Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville

2Laboratory of Cellular and Molecular Biology, FST-UMNG, B.P 69 Brazzaville

3Laboratory of Geomatics and Applied Tropical Ecology


International Journal of Environmental Bioremediation & Biodegradation. 2023, Vol. 11 No. 1, 1-8
DOI: 10.12691/ijebb-11-1-1
Copyright © 2023 Science and Education Publishing

Cite this paper:
Gatse Elgie Viennechie, Mboukou Kimbatsa Irène Marie Cecile, Onyankouang Isaac Samuel, Morabandza Cyr Jonas, Goma Tchimbakala Joseph, Nguimbi Etienne. Monitoring of Cellulase and Biosurfactant Production in Bacteria Isolated from the Likouala Peat Bog (Republic of Congo). International Journal of Environmental Bioremediation & Biodegradation. 2023; 11(1):1-8. doi: 10.12691/ijebb-11-1-1.

Correspondence to: Morabandza  Cyr Jonas, Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville. Email: moressejonas@gmail.com

Abstract

The production of cellulases and biosurfactants of isolated bacteria from the Likouala peat bog (Congo-Brazzaville) was studied. Growth and production of cellulases and biosurfactants were monitored by conventional techniques on Mossel and TSB media enriched with petroleum or vegetable oil. Correlation coefficients between cellulases and biosurfactants production were calculated with Microsoft Excel 2016 software. The results showed optical densities from 0.634 to 1; diameters of translucent halos from 1.4 to 5.8cm and emulsification indices of isolates from 0 to 80%. The production time of cellulases and biosurfactants was 24 to 96 h with an optimum at 72h for biosurfactants depending on the isolate. The isolate correlation index were: r=0.74 and r=0.53 for RE 20; r=0.87 and r=0.60 for RE 21 and r=0.96 and 0.91 for RE 24 on Mossel; r=0.77 and r=0.61 for BV1; r=0.96 and r=0.95 for BV2; r=0.95 and r=0.52 for BV3 and r=0.92 and r=0.83 for BV4 on TSB enriched with petroleum; r=0.95 and r=0.86 for BV6; 0.54 and 0.57 for BV7 and r=0.84 and r=0.95 for BV9 on TSB enriched with vegetable oil respectively for the production of cellulases and biosurfactants. The Likouala peat bog area is rich in hydrocarbonoclast bacteria that produce cellulases and biosurfactants from peat.

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