Qualitative and Quantitative Assessment of a Surfactin biosurfactant in the Bioaugmentation of Crude-oil Contaminated Soil in Garages in the Republic of Congo

Paola Sandra Elenga Wilson¹, Gabriel Judicael Okeni-Boba¹, Christian Aimé Kayath^1, , Kiele Molingo Mbemba¹, Etienne Nguimbi¹ and Gabriel Ahombo¹

¹Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien NGOUABI, BP. 69, Brazzaville, République du Congo

Cite this paper:
Paola Sandra Elenga Wilson, Gabriel Judicael Okeni-Boba, Christian Aimé Kayath, Kiele Molingo Mbemba, Etienne Nguimbi and Gabriel Ahombo. Qualitative and Quantitative Assessment of a Surfactin biosurfactant in the Bioaugmentation of Crude-oil Contaminated Soil in Garages in the Republic of Congo. International Journal of Environmental Bioremediation & Biodegradation. 2022; 10(1):1-11. doi: 10.12691/ijebb-10-1-1

Abstract

The objective of this research was to perform a survey of different garages in seven (7) districts of the Republic of Congo and to further study the influence of biosurfactant produced by isolate M28 (Bacillus subtilis based on fib-E gene identification and RNA 16S gene sequences) and a consortium of selected bacteria on soil remediation. A total of 140 garages were found in Brazzaville. The evaluated soil samples showed that total petroleum hydrocarbons (TPH) content varied from 165 to 206 g/kg of soil. Unfortunately, in the Republic of Congo, the regulatory intervention values for TPH have yet to be determined. Four experiments were conducted in order to improve bioaugmentation. First experiment: wild contaminated soil and bacteria: The second experiment included wild contaminated soil, bacteria, and a biosurfactant; the third experiment included wild contaminated soil, a consortium, and a biosurfactant. Surfactin, a biorsurfactant produced by M28, was used to enhance bioaugmentation of wild contaminated soil from a garage from Bacongo.

Keywords:
Bacillus bioaugmentation biosurfactants garages

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