1Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien NGOUABI, BP. 69, Brazzaville, République du Congo
International Journal of Environmental Bioremediation & Biodegradation.
2022,
Vol. 10 No. 1, 1-11
DOI: 10.12691/ijebb-10-1-1
Copyright © 2022 Science and Education PublishingCite this paper: Paola Sandra Elenga Wilson, Gabriel Judicael Okeni-Boba, Christian Aimé Kayath, Kiele Molingo Mbemba, Etienne Nguimbi, Gabriel Ahombo. Qualitative and Quantitative Assessment of a Surfactin
biosurfactant in the Bioaugmentation of Crude-oil Contaminated Soil in Garages in the Republic of Congo.
International Journal of Environmental Bioremediation & Biodegradation. 2022; 10(1):1-11. doi: 10.12691/ijebb-10-1-1.
Correspondence to: Christian Aimé Kayath, Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien NGOUABI, BP. 69, Brazzaville, République du Congo. Email:
chriskayath@yahoo.frAbstract
The objective of this research was to perform a survey of different garages in seven (7) districts of the Republic of Congo and to further study the influence of biosurfactant produced by isolate M28 (Bacillus subtilis based on fib-E gene identification and RNA 16S gene sequences) and a consortium of selected bacteria on soil remediation. A total of 140 garages were found in Brazzaville. The evaluated soil samples showed that total petroleum hydrocarbons (TPH) content varied from 165 to 206 g/kg of soil. Unfortunately, in the Republic of Congo, the regulatory intervention values for TPH have yet to be determined. Four experiments were conducted in order to improve bioaugmentation. First experiment: wild contaminated soil and bacteria: The second experiment included wild contaminated soil, bacteria, and a biosurfactant; the third experiment included wild contaminated soil, a consortium, and a biosurfactant. Surfactin, a biorsurfactant produced by M28, was used to enhance bioaugmentation of wild contaminated soil from a garage from Bacongo.
Keywords