Efficacy of a Novel, Dentin-Derived, Xenogeneic Bone Graft Material in a Clinically Relevant PorcineModel: A Comparative Study

Lari Sapoznikov¹ and Martin Humphrey^2,

¹Private Practice, Tel Aviv, Israel

²Private Consultant, Munich, Germany

Cite this paper:
Lari Sapoznikov and Martin Humphrey. Efficacy of a Novel, Dentin-Derived, Xenogeneic Bone Graft Material in a Clinically Relevant PorcineModel: A Comparative Study. International Journal of Dental Sciences and Research. 2025; 13(3):53-59. doi: 10.12691/ijdsr-13-3-2

Abstract

Tooth dentin-derived autogenous bone graft material is very effective for dental bone defect repair but is limited by the need for sufficient material. A xenogeneic material with retained organic matrix, Ivory Dentin Graft, has thus been developed. The properties of this material in comparison to a bone-derived material were examined in a clinically relevant porcine model. Using a split-mouth design, two types of bone defects were created and grafted using either Ivory Dentin Graft or a bone-derived material with retained organic component. The extraction site of an extracted mandibular incisor modelled post-extraction socket preservation, while a mandibular sub-periosteal pouch modelled bone augmentation in procedures such as sinus lifting. At 10 weeks after grafting, when new bone growth and site remodeling is active, the graft sites were solid, dense and stable with no sign of loose particles with both materials. The dentin-derived material was distinguished by having a much higher mean percentage of intimate contact between the graft material and new bone growth (77.5% versus 45.5%) which was statistically different (p < 0.001, paired t-test, 2-tailed). This confirms that Ivory Dentin Graft retains the key property of autologous dentin, of forming an ankylosed network with new bone ingrowth which is key for early and maintained graft site stability.

Keywords:
bone graft bone dentin tooth material orthopedic dental xenogeneic osteoinductive ankylosis animal model implant

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Sapoznikov, L., Humphrey, M. Progress in Dentin-Derived Bone Graft Materials: A New Xenogeneic Dentin-Derived Material with Retained Organic Component Allows for Broader and Easier Application. Cells, 13(21), 1806. Oct 2024.
[2]	Binderman, I.; Hallel, G.; Nardy, C.; Yaffe, A.; Sapoznikov, L. A Novel Procedure to Process Extracted Teeth for Immediate Grafting of Autogenous Dentin. J. Interdiscipl. Med. Dent. Sci., 2, 154. Oct 2014.
[3]	Murata, M.; Nezu, T.; Takebe, H.; Hirose, Y.; Okubo, N.; Saito, T.; Akazawa, T. Human dentin materials for minimally invasive bone regeneration: Animal studies and clinical cases. J. Oral Biosci., 65(1), 13–18. Nov 2023.
[4]	Cervera-Maillo, J.M.; Morales-Schwarz, D.; Morales-Melendez, H.; Mahesh, L.; Calvo-Guirado, J.L. Autologous Tooth Dentin Graft: A Retrospective Study in Humans. Medicina, 58(1), 56. Dec 2021.
[5]	Inchingolo, A.M.; Patano, A.; Di Pede, C.; Inchingolo, A.D.; Palmieri, G.; de Ruvo, E.; Campanelli, M.; Buongiorno, S.; Carpentiere, V.; Piras, F.; et al. Autologous Tooth Graft: Innovative Biomaterial for Bone Regeneration. Tooth Transformer® and the Role of Microbiota in Regenerative Dentistry. A Systematic Review. J. Funct. Biomater., 14(3), 132. Feb 2023.
[6]	Kim, Y.K.; Kim, S.G.; Byeon, J.H.; Lee, H.J.; Um, I.U.; Lim, S.C.; Kim, S.Y. Development of a novel bone grafting material using autogenous teeth. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontol. 109(4), 496–503. Apr 2010.
[7]	Kim, Y.; Um, I.; Murata, M. Tooth Bank System for Bone Regeneration - Safety Report. J. Hard Tissue Biol., 23(3), 371–376. Jul 2014.
[8]	Galindo-Moreno, P.; Hernández-Cortés, P.; Mesa, F.; Carranza, N.; Juodzbalys, G.; Aguilar, M.; O'Valle, F. Slow resorption of anorganic bovine bone by osteoclasts in maxillary sinus augmentation. Clin. Implant Dent. Relat. Res., 15(6), 858–866. Dec 2013.
[9]	Trzaskowska, M.; Vivcharenko, V.; Przekora, A. The Impact of Hydroxyapatite Sintering Temperature on Its Microstructural, Mechanical, and Biological Properties. Int. J. Mol. Sci., 24(6), 5083. Mar 2023.
[10]	Miron, R.J.; Fujioka-Kobayashi, M.; Pikos, M.A.; Nakamura, T.; Imafuji, T.; Zhang, Y.; Shinohara, Y.; Sculean, A.; Shirakata, Y. The development of non-resorbable bone allografts: Biological background and clinical perspectives. Periodontol 2000, 94(1), 161–179. Feb 2024.
[11]	Dłucik, R.; Orzechowska-Wylęgała, B.; Dłucik, D.; Bogus, K. Histological examination of tooth-derived biomaterials obtained from different devices. Expert Rev. Med. Devices, 20(11), 979–988. Jul-Dec 2023.
[12]	Sapoznikov, L.; Haim, D.; Zavan, B.; Scortecci, G.; Humphrey, M.F. A novel porcine dentin-derived bone graft material provides effective site stability for implant placement after tooth extraction: A randomized controlled clinical trial. Clin. Oral Investig., 27(6), 2899–2911. Jun 2023.
[13]	Romasco, T.; Tumedei, M.; Inchingolo, F.; Pignatelli, P.; Montesani, L.; Iezzi, G.; Petrini, M.; Piattelli, A.; Di Pietro, N. A Narrative Review on the Effectiveness of Bone Regeneration Procedures with OsteoBiol® Collagenated Porcine Grafts: The Translational Research Experience over 20 Years. J. Funct. Biomater., 13(3), 121. Aug 2022.
[14]	Wang, S.; Liu, Y.; Fang, D.; Shi, S. (2007). The miniature pig: a useful large animal model for dental and orofacial research. Oral diseases, 13(6), 530–537. Nov 2007.
[15]	Li, Y.; Chen, S. K.; Li, L.; Qin, L.; Wang, X. L.; Lai, Y. X. (2015). Bone defect animal models for testing efficacy of bone substitute biomaterials. Journal of orthopaedic translation, 3(3), 95–104. Jun 2015.
[16]	Zhang, Z.; Gan, Y.; Guo, Y.; Lu, X.; Li, X. Animal models of vertical bone augmentation (Review). Experimental and therapeutic medicine, 22(3), 919. Sep 2021.
[17]	Buser, D.; Hoffmann, B.; Bernard, J. P.; Lussi, A.; Mettler, D.; Schenk, R. K. Evaluation of filling materials in membrane--protected bone defects. A comparative histomorphometric study in the mandible of miniature pigs. Clinical oral implants research, 9(3), 137–150. Jun 1998.
[18]	Jensen, S. S.; Broggini, N.; Hjørting-Hansen, E.; Schenk, R.; Buser, D. Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs., Clinical oral implants research, 2006, 17(3), 237–243. Jun 2006.