Androgenic and Antioxidant Effects of Ethanolic Extract of Strychnos camptoneura (longaniaceae) Trunk Bark in Male Wistar Rats

Akassa Herman^1, , Peneme Bonaventure Max Lazare^{1, 2}, Etou Ossibi Wilfrid Arnauld^{1, 2}, Morabandza Cyr Jonas¹, Kanga Marie Roselyne¹, Goma Dinouvanza Lèges Aphrodite¹ and Abena Ange Antoine^{1, 3}

¹Laboratory of Biochemistry and Pharmacology, Heath Sciences Faculty, Marien Ngouabi University P.0.Box. 69, Brazzaville (Congo)

²Animal Physiology Laboratory, Sciences and Technical Faculty, Marien Ngouabi University, P.O. Box 69, Brazzaville, (Congo)

³Denis SASSOU N’GUESSO University, Kintélé, Republic of Congo

Cite this paper:
Akassa Herman, Peneme Bonaventure Max Lazare, Etou Ossibi Wilfrid Arnauld, Morabandza Cyr Jonas, Kanga Marie Roselyne, Goma Dinouvanza Lèges Aphrodite and Abena Ange Antoine. Androgenic and Antioxidant Effects of Ethanolic Extract of Strychnos camptoneura (longaniaceae) Trunk Bark in Male Wistar Rats. American Journal of Pharmacological Sciences. 2023; 11(1):8-14. doi: 10.12691/ajps-11-1-2

Abstract

The main purpose of the present study was to evaluate the androgenic and antioxydant effects of ethanolic extract of Strychnos camptoneura trunk bark in Wistar rats. For this purpose, 20 rats were randomly arranged in 4 lots of 5 animals each and received respectively for lots 1 and 2 distilled water and testosterone enanthate and lots 3 and 4 treated with ethanolic extract of Strychnos camptoneura (100 and 250 mg /kg). It appears from this study that the administration of ethanolic extract of Strychnos camptoneura orally at the doses studied causes significant increases in body weight gain (p < 0.05; p < 0.01 and p < 0.001) in rats in contrast to the animals of the negative control lot that received distilled water. Administration of Strychnos camptoneura extract at the doses studied did not induce any significant change (P > 0.05) in the relative weights of testes, vas deferens, seminal vesicles, and penis compared to negative control rats. Furthermore administration of ethanolic extract of Strychnos camptoneura at the doses studied induced a significant increase (p < 0.01 and p < 0.001) in serum and testicular testosterone levels in rats compared to those treated with distilled water. Chemical analyses performed in this study revealed the presence of total polyphenols (TPP) and flavonoids in the ethanolic extract of Strychnos camptoneura. These results suggest that the ethanolic extract of Strychnos camptoneura would have androgenic and antioxidant properties and would justify its use in traditional Congolese medicine for the treatment of male infertility.

Keywords:
Strychnos camptoneura androgenic hypogonadisme and antioxidant

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

References:

[1]	Bhasin S, Cunningham GR, Hayes FJ. Testosterone therapy in men with androgen deficiency syndromes: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab. 95: 2536-59, 2010.
[2]	Boehm U, Bouloux PM, Dattani MT. 2 Expert consensus document: European Consensus Statement on congenital hypogonadotropic hypogonadism--pathogenesis, diagnosis and treatment. Nat Rev Endocrinol. 11: 547-64, 2015.
[3]	Ngoula F, Guemdjo T M, Kenfack A, Tadondjou CD, Nouboudem S, Ngoumtsop H, TsafackB, Teguia A, Kamtchouing P, Galeotti M, Tchoumboue J. Effects of heat stress on some reproductive parameters of male cavie (Cavia porcellus) and mitigation strategies using guava (Psidium guajava) leaves. Journal of Thermal Biology. 64: 67-72, 2017.
[4]	Kenfack A, Akono NE, Ngoula F, Nounamo JG.A, Kamtchouing P, Tchoumboue J. Post Exposure Effects of Propoxur (Agricultural Pesticide) on Male Fertility in Wistar Rat. Iranian Journal of Toxicology. 12 (3): 21-27, 2018.
[5]	Akassa H. Aphrodisiac and androgenic effects of the aqueous extract of the trunk bark of pausinystalia yohimbe k schum (rubiaceae) in wistar rats PP 106 UMNG, 2019.
[6]	Zena F H. Study the effect of Eruca sativa leaves extract on male fertility in albino mice. Journal of Al-Nahrain University, 16(1): 143-146, 2013.
[7]	Ngandjui A, Ngaha NM, Kenmogne H, Koloko BL, Massoma L.D. Evaluation of the fertility activity of the aqueous leaves extract of Zanthoxylum macrophylla (Rutaceae) on male rats. J Phytopharmacol. 6 (5): 277-281, 2017.
[8]	Akassa H, Peneme BM, Ondélé R, Etou Ossebi AW, Tamboura HH, Abena AA. Androgenic activity of the aqueous extract of the stem barks of Pausinystalia yohimbe kschum (Rubiaceae) in wistar rats. European Journal of Biotechnology and Bioscience 7 (4): 83-87, 2019.
[9]	Talla C, Fogang Vougmo JC, NNJ, and Mpondo M. Evaluation of the Androgenic Properties of the Aqueous Extract of the Trunk Bark of Spathodea Campanulata P. Beauv. (Bignoniaceae) in Male Rats Health Sci. Dis: 22 (3): 60-68, 2021.
[10]	Akassa H, Peneme BML, Morabandza CJ, Etou 0ssibi AW, and Abena AA. Evaluation of the androgenic potentialities of the hydro-ethanolic extract of the trunk barks of Strychnos camptoneura (longaniaceae) in the wistar rat. world journal of pharmacy and pharmaceutical science. 11: 145-156, 2022.
[11]	Gayrard V. Physiology of mammalian reproduction. National Veterinary School. Toulouse, France, 198p, 2007.
[12]	Gonzales GF, Cordova A, Vega K, Chung A, Villena A, Gonez C. Effect of Lepidium meyenii (Maca), a root with aphrodisiac and fertility-enhancing properties, on serum reproductive hormone levels in adult healthy men. J Endocrinol. 8 (1): 163-176, 2003.
[13]	Brand-Williams W, Cuvelier M.E, and Berset C. Use of free radical method to evaluate antioxidant activity. Technology. 28: 25-30, 1995.
[14]	Huang DB, Prior RL The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry; 53: 1841-1856, 2005.
[15]	Waterhouse A L. Determination of total phenolics. Current Protocols in Food Analytical Chemistry; I1.1.1-I1.1.8, 2001.
[16]	Subhasree B, Baskar R, Keerthana R. L, Susan R, & Rajasekran P.Evaluation of antioxidant potential in selected green leafy vegetables. Food Chemistry; 115: 1213-1220, 2009.
[17]	Akono N E, Moundipa PF E, Beboy NS, Monsees T K., Carreau S. Etude de I. ‘effet androgénique de I ‘extrait au méthanol de Basella alba L. (Basellaceae) sur la fonction de reproduction du rat male, 17, N~ 129-133, 2007.
[18]	Watcho P, Hermine M, Watio M, Wankeu-Nya E, Ngadjuil P D, Defo P, Alango Nkeng-Efouet TBN, and Kamanyil A. Androgenic effects of aqueous and methalic extract of ficus asperifolia in male Wistar rats BMC complementary and alternative medicine 17(42): 1-9, 2017.
[19]	Akassa H, Samlan Okemy AN, Peneme BM L, Goma Dinouvanza A. L, Kanga MR, and Abena AA. Spermatogenic and antiradical activities of the hydro-ethanolic extract of Strychnos camptoneura (longaniaceae) trunk barks in wistar rats. World Journal of Advanced Research and Reviews. 17(01): 386-394, 2023.
[20]	Nelisiwe PM, Jeremiah O U, and Sogolo L L. Phytochemical content, antioxidant activities and androgenic properties of four South African medicinal plants J Herbmed Pharmacol.; 9(3): 245-256, 2020.
[21]	Klimczak I, Małecka M, Szlachta M, Gliszczyńska Ś. Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. J Food Compost Anal. 20(3-4): 313-22, 2007.