Toxicity and Relaxant Effects of the Aqueous Extract of Stereospermum kunthianum Cham. (Bignoniaceae) Leaves on Uterine Horn Contractility in Rats

Dabiré Anankpètinan Prosper^{1, 2,} , Somé Andouormwine Abel², Sawadogo Stanislas² and Bonkoungou Edouard Windpouiré²

¹Ecole Normale Supérieure, 01 BP 1757 Ouagadougou 01, Burkina Faso

²Laboratory of animal physiology, Department of Animal Biology and Physiology, University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03 Burkina Faso

Cite this paper:
Dabiré Anankpètinan Prosper, Somé Andouormwine Abel, Sawadogo Stanislas and Bonkoungou Edouard Windpouiré. Toxicity and Relaxant Effects of the Aqueous Extract of Stereospermum kunthianum Cham. (Bignoniaceae) Leaves on Uterine Horn Contractility in Rats. American Journal of Pharmacological Sciences. 2022; 10(1):47-52. doi: 10.12691/ajps-10-1-8

Abstract

Stereospermum kunthianum is a plant used in traditional medicine of Burkina Faso. Ethnobotanical survey showed that this plant have many virtues but the toxicity and the effect of the leaves part on uterine contraction are not yet documented. To fill this gap, we investigated the toxicity effect on mice and the pharmacological effect of Stereospermum kunthianum on the contraction of rat isolated uterine horns. The acute and subacute toxicities were studied firstly. Secondary, the uterine segments were isolated and exposed to different concentrations of the aqueous extract of Stereospermum kunthianum leaves, oxytocin and KCl. The results showed that Stereospermum kunthianum aqueous extract decreased the amplitude and frequency of contractions. IC₅₀ values were 1.46 and 2.03 respectively for amplitude and frequency. Our study revealed that the extract inhibited uterine contractions induced by oxytocin and potassium chloride (KCl). These data suggest that Stereospermum kunthianum active compound could be used for calming uterine contractions. The effect of Stereospermum kunthianum showed that it could be useful to fight against diseases that caused uterotonic actions. It may be useful to prevent preterm birth and pain caused by menstruation, as indicated by its traditional use.

Keywords:
Stereospermum kunthianum toxicity relaxant effects uterine contractility rat

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References:

[1]	Igoli, J.O., Ogaji, O.G., Tor-Anyiin, T.A., Igoli, N.P., “Traditional medicine practice amongst the Igede People of Nigeria. Part II,” Afr. J. Tradit. Complement. Altern. Med., vol. 2, no. 2, pp. 134-152, 2005.
[2]	Nacoulma/Ouédraogo, O.G., “Plantes médicinales et pratiques médicinales traditionnelles au Burkina: cas du plateau central,” Université de Ouagadougou, 1996.
[3]	Egua, M.O., Okwoche, O.J., Nwinyi, F.C., Monday, O.M., Ganiyat, A.M., Abalaka, S.E., Garba, Mikail, H., Dezi, A.D., Mohammed, A., “The sub acute toxicity evaluation of Stereospermum kunthianum aqueous methanolic stem bark extract,” J. Biosci. Med., vol. 9, pp. 71-86, 2021.
[4]	Ching, F.P., Omogbai, E.K.I. and Otokiti, I.O., “Aqueous stem bark extract of Stereospermum kunthianum (cham, sandrine petit) protects against generalized seizures in pentylenetetrazole and electro-convulsive models in rodents.,” Afr. J. Tradit. Complement. Altern. Med., vol. 6, no. 4, pp. 54-548, 2009.
[5]	Awang, A.F.I., Ferdosh, S., Sarker, M.Z.I., Sheikh, H.I., Ghafoor, K. and Yunus, K., “Stereospermum fimbriatum as a potential source of phytochemicals: A review of Stereospermum Genus” Curr. Pharm. Biotechnol., vol. 17, pp. 1024-1035, 2016.
[6]	Dabiré, A.P., Ouédraogo, Y., Somé, A.A., Sawadogo, S., Ouédraogo, I., Ilboudo, M.E., Belemtougri, G.R., “relaxant effects of the aqueous extract of Excoecaria grahamii (Euphorbiaceae) leaves on uterine horn contractility in Wistar Rats,” Hindawi, BioMed Res. Int., vol. 2021, p. 8, 2021.
[7]	Caligioni, C.S., “Assessing reproductive status / stages in mice,” Curr. Protoc. Neurosci., Append. 4I, vol. 48, pp. 1-8, 2009.
[8]	Kharchoufa, L.B., Mohamed, B., Noureddine, A., El Soufiane, A., Asmae, Y., Amal, Choukri, M. Mekhfi, H., Elachouri, M., “Acute and subacute toxicity studies of the aqueous extract from Haloxylon scoparium Pomel ( Hammada scoparia ( Pomel )) by Oral Administration in Rodents,” Biomed Res. Int., vol. 2020, p. 11 p, 2020.
[9]	OECD, “Guideline for testing of chemicals. 423 acute oral toxicity – acute toxic class method,” 2001.
[10]	Okpo, S.O. and Ching, F.P., “Sub-acute toxicity studies on the aqueous extract of Stereospermum kunthianum (Cham , Sandrine Petit) stem bark,” J. Pharm. Bioresour., vol. 10, no. 2, pp. 38-44, 2013.
[11]	Khan, R.N., Matharoo-Ball, B., Arulkumaran, S., and Ashford, M.L., “Potassium channels in the human myometrium,” Exp.Physiol, vol. 86, no. 2, pp. 25-264, 2001.
[12]	Alotaibi, M., “The physiological mechanism of uterine contraction with emphasis on calcium ion,” Calcium Signal., vol. 1, no. 2, pp. 70-75, 2014.
[13]	Jaafarpour, M., Hatefi, M., Khani, A. and Khajavikhan, J., “Comparative Effect of Cinnamon and Ibuprofen for Treatment of Primary Dysmenorrhea: A Randomized Double-Blind Clinical Trial,” J. Clin. Diagnostic Res., vol. 9, no. 4, pp. 4-7, 2015.
[14]	Thakur, P. and Pathania, A. R., “Relief of dysmenorrhea – A review of different types of pharmacological and non-pharmacological treatments,” Mater. Today Proc., pp. 1-6, 2021.
[15]	Dawood, M. Y., “Nonsteroidal Anti-Inflammatory Drugs and Changing Attitudes toward Dysmenorrhea,” Am. J. Med., vol. 84, pp. 23-29, 1988.
[16]	Bafor, E., Eyegbagharen, T., Ochoyama, E. and Idu, M., “Ex-vivo inhibition of uterine contractility by the stem bark extracts of Omphalocarpum procerum P. Beauv. (Sapotaceae) in mouse models,” J. Pharm. Pharmacogn. Res., vol. 8, no. 2, pp. 92-104, 2020.
[17]	Lloyd K. B. and Hornsby, L. B., “Complementary and Alternative Medications for Women’s Health Issues,” Nutr. Clin. Pract., vol. 24, no. 5, pp. 589-608, 2009.
[18]	Ching, F. P., Omogbai, E. K. I., Ozolua, R. I. and Okpo, S. O., “Analgesic activity of aqueous extract of Stereospermum kunthianum (Cham, Sandrine Petit) stem bark,” Acta Pol. Pharm., vol. 66, pp. 83-88, 2009.
[19]	Alotaibi, M. “The effect of cinnamon extract on isolated rat uterine strips,” Reprod. Biol., vol. 16, no. 1, pp. 27-33, 2016.
[20]	Amaechina F. C. and Bafor, E. E., “In vitro inhibitory effect of methanol leaf extract of Stachytarpheta jamaicensis (Verbenaceae) on non-pregnant rat uterus,” Trop. J. Pharm. Res., vol. 15, pp. 2557-2562, 2016.
[21]	Ferreira, J. J. et al., “Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the Na+ -activated K+ channel, Slo2.1,” J. Physiol., vol. 1, pp. 137-149, 2019.
[22]	Munglue,P., Eumkep, G., Wray, S. and Kupittayanant, S., “The effects of watermelon (Citrullus lanatus) extract and L-citruline on rat uterine contractility,” Reprod. Sci., vol. 00, no. 0, pp. 1-12, 2013.
[23]	Arrowsmith, S., “Oxytocin and vasopressin signalling and myometrial contraction,” Physiology, vol. 13, pp. 62-70, 2020.