Evaluation of the Anticonvulsive Effects of the Aqueous Extract of the Roots of Crossopteryx febrifuga in Mice

Bassoueka D’Avila Judicaël^1, , Peneme Bonaventure Max Lazare¹ and Abena Ange Antoine²

¹Laboratory of Pharmacodynamics and Experimental Physiopathology (L2PE), Faculty of Sciences and Techniques, Marien Ngouabi University, BP. 69 Brazzaville, Congo

²Laboratory of Biochemistry and Pharmacology, Faculty of Health Sciences, Marien Ngouabi University, BP: 69 Brazzaville, Congo

Cite this paper:
Bassoueka D’Avila Judicaël, Peneme Bonaventure Max Lazare and Abena Ange Antoine. Evaluation of the Anticonvulsive Effects of the Aqueous Extract of the Roots of Crossopteryx febrifuga in Mice. American Journal of Pharmacological Sciences. 2022; 10(1):26-30. doi: 10.12691/ajps-10-1-5

Abstract

The present study aimed to evaluate the anticonvulsant and sedative effects of the aqueous extract of Crossopteryx febrifuga. Two pharmacological tests such induction of convulsions by Picrotoxin and Strychnine were carried out to evaluate the anticonvulsant effect of C. febrifuga roots. The two other tests performed for the sedative effect involved the motor activity test and the barbiturate sleep potentiation test in mice. The aqueous extract of Crossopteryx febrifuga roots at a dose of 200 mg/kg showed a significant increase (**p < 0.01 and *p < 0.05) in the time to onset of seizures induced by Picrotoxin and a non-significant increase in the time to onset of strychnine-induced seizures in mice. The extract caused a non-significant decrease in the duration of convulsions in mice at doses of 100 and 200 mg/kg. Crossopteryx febrifuga extract had no effect on motor activity. However, at a dose of 200 mg/kg the extract caused a significant increase (**p < 0.01 and *** p < 0.001) in the time to onset of sleep.

Keywords:
anticonvulsant motor activity Crossopteryx febrifuga mice Picrotoxin

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References:

[1]	Maiha B.B., Magaji M.G., Yaro A.H., Hamza A.H., Ahmed S.J., Magaj A.R. (2009). Anticonvulsant studies on cochlospermum tinctorium and Paullinia pinnata extracts in laboratory animals. Nigerian Journal of Pharmaceutical Sciences 8: 102-108.
[2]	Vyawahare N.S., Khandelwal A.R., Batra V.R., Nikam A.P. (2007). Herbal anticonvulsants. Journal of Herbal Medicine and Toxicology 1: 9-14.
[3]	Marjan N.A., Zamansoltani F., Torabinejad B. (2009). Antiepileptic effects of quinine in the pentylenetetrazole model of seizure. Seizure 8: 129-132.
[4]	Reddy D.S. (2005). Pharmacotherapy of catamenial epilepsy. Indian Journal of Pharmacology 37: 288-293.
[5]	Hema B., Bhupendra S., Mohamed Saleem T.S., Gauthaman K. (2009). Anticonvulsant effect of Drosera burmannii Vahl. International Journal of Applied Research in Natural Products 2: 1-4.
[6]	Ezekiel I., Mabrouk M.A., Ayo J.O., Goji A.D., Okpanachi A.O., Mohammed A., Tanko Y. (2010). Study of the effect of hydro-ethanolic extract of Commiphora africana (stem-bark) on sleeping time and convulsion in mice. Asian Journal of Medical Sciences 2: 85-88.
[7]	Wannang N.N., Anuka J.A., Kwanashie H.O., Gyang S.S., Auta A. (2008). Anti-seizure activity of the aqueous leaf extract of Solanum nigrum L. (Solanaceae) in experimental animal. African Health Sciences 8: 74-79.
[8]	Joy P.P., Thomas J., Mathew S., Skaria B.P. (2001); Medicinal Plants Tropical Horticulture. Vol-2, Calcutta: Naya Prokashan.
[9]	Hosseinzadeh H., Khosravan V. (2002). Anticonvulsant effects of aqueous and ethanolic extracts of Crocus sativus L. stigmas in mice. Archives of Internal Medicine 5: 44-47.
[10]	Hosseinzadeh H., Parvardeh S. (2004). Anticonvulsant effects of thymoquinone, the major constituent of Nigella sativa seeds in mice. Phytomedicine 11: 56-64.
[11]	Quintans-Júnior L.J., Almeida J.R., Lima J.T., Nunes X.P., Siqueira J.S., Gomes de Oliveira L.E., Almeida R.N., Athayde-Filho P., Barbosa-Filho J.M. (2008a). Plants with anticonvulsant properties-a review. Revista Brasileira de Farmacognosis 18: 798-819.
[12]	Quintans-Júnior L.J., Souza T.T., Leite B.S., Lessa N.M.N., Bonjardim L.R., Santos M.R.V., Alves P.B., Blank A.F., Antoniolli A.R. (2008b). Phythochemical screening and anticonvulsant activity of Cymbopogon winterianus Jowitt (Poaceae) leaf essential oil in rodents. Phytomedicine 15: 619-624.
[13]	Bassoueka D.J., Taiwe Sotoing G., Nsonde Ntandou G.F. et Ngo Bum E. (2016). Anticonvulsant activity of the decoction of Crossopteryx febrifuga in mice. International Journal of Science and Research 5(3): 112-116.
[14]	Bassoueka D.J., Loufoua B.A.E., Abena A.A., (2019). Assessment of anxiolytic abd antidepressant effects of Crossopteryx febrifuga in mice. International journal of science and research 7: 2319-7064.
[15]	Bassoueka D. J., Loembet E.C., Mavethy A.B.E., Loufoua O.B., Abena1 A.A. (2022). Anticonvulsant and sedative effects assessment of aqueous extract of Crossopteryx febrifuga seeds in mice. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) 1: 27-31.
[16]	Adesina S.K. (1982). Studies on some plants used as anticonvulsants in Amerindian and African traditional medicine. Fitoterapia 53: 147-162.
[17]	Mao S.C., Li Z.Y., Li G. (2007). Studies on antioxidation activity of three plants of Desmodium. Yunnan Daxue Xue Bao 29: 393-397.
[18]	Poole K., Moran N., Bell G., Solomon J., Kendall S., McCarthy M., McCormick D., Nashef L., Johnson A., Sander J., Shorvon S. (2000). Patients' perspectives on services for epilepsy: a survey of patient satisfaction, preferences and information provision in 2394 people with epilepsy. Seizure 9: 551-558.
[19]	Ngo Bum I., Temkou Ngoupaye G., Dimo T. (2008). The anticonvulsant and sedative properties of stems of Cissus quadrangularis in mice. African Journal of Pharmacy and Pharmacology 2(3): 42-47.
[20]	Ngo Bum E., Taiwe G.S., Nkaissa L.A., Moto F.C.O., Seke Etet P.F., Hiana I.R., T., Rouyatou, Papa Seyni, Rakotonirina A., Rakotonirina S.V. (2009). Validation of anticonvulsant and sedative activity of six medicinal plants. Epilepsy and Behavior 14: 454-458.