Effects of Ficus exasperata Root Bark Aqueous and Ethanolic 70 % Extracts on Urinary Excretion in Wistar Rat

Kouao Augustin Amonkan¹, Brou André Konan^1, and Kacou Jules Marius Djétouan¹

¹UPR-Nutrition and Pharmacology, Laboratory of Biology and Health, UFR-Biosciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire

Cite this paper:
Kouao Augustin Amonkan, Brou André Konan and Kacou Jules Marius Djétouan. Effects of Ficus exasperata Root Bark Aqueous and Ethanolic 70 % Extracts on Urinary Excretion in Wistar Rat. American Journal of Pharmacological Sciences. 2022; 10(1):20-25. doi: 10.12691/ajps-10-1-4

Abstract

The roots of Ficus exasperata Valh (Moraceae) are commonly used in traditional medicine to treat various pathologies including edema and arterial hypertension. Also, this study was conducted to assess the effects of two extracts of the root bark of this medicinal plant on urinary excretion in rats. To do this, 12 groups of 6 water-overloaded rats (50 ml/kg) were treated separately with 0.9% NaCl solution, FURO 5 mg/kg, FEae (15-100 mg/kg) and FEee (15-100 mg/kg). Excreted urine was collected at regular intervals of 2 h for 8 h to determine urinary excretion volume (UEV). Like furosemide, the two plant extracts (FEae and FEee) caused significant urinary excretion in water-overloaded rats. The most marked effect in terms of UEV was observed after the administration of FEae 15 mg/kg. At this dose, an EUV of 76 ± 0.21% and higher than that of FURO 5 mg/kg (59 ± 0.01%) was obtained 6 h after the treatment of the animals. These recorded EUV values were significantly higher at p < 0.001 than that of 0.9 % NaCl saline solution. Ficus exasperata root bark extracts induced significant increases in urinary excretion in Wistar rats. These results could justify the empirical use of this plant as a diuretic in the treatment of edema and hypertension. However, in order to better understand the physiological mechanisms underlying this action, additional studies must be carried out.

Keywords:
Ficus exasperata furosemide diuretic urinary excretion arterial hypertension edema

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References:

[1]	Monkam-Mbouende, Y., “L’hypertension artérielle en Afrique Noire”, Médical Digest, 15. 2-8. Jan.1989.
[2]	Bertrand, E., “Evolution épidémiologique des maladies cardio-vasculaires dans les pays en voie de développement”, Archives des Maladies du Coeur et des Vaisseaux, 90 (7). 981-985. Jan. 1997.
[3]	OMS, Comment éviter une crise cardiaque ou un accident vasculaire cérébral. Protégez-vous avant qu’il soit trop tard, OMS, Genève, Switzerland, 2006, 44.
[4]	Perucca, J., Rein, vasopressine et pression arterielle: importance de la Concentration de l'urine et du rythme nycthemeral d'excretion d'eau et de sodium, Doctorat de Physiologie et Physiopathologie, Universitée Paris IV, Pierre et Marie Curie, Paris, France, 2008, 218.
[5]	Whitworth, J.A. and WHO-ISH, “World Health Organization (WHO)/International Society of Hypertension (ISH) statement on management of hypertension”, Journal of Hypertension, 21 (11). 1983-1992. Nov 2003.
[6]	Kearney, P.M., Whelton, M., Reynolds, K., Whelton, P.K. and He, J., “Worldwide prevalence of hypertension: a systematic review”, Journal of Hypertension, 22 (1). 11-19. Jan 2004.
[7]	Sofowara, A., Medicinal Plants and Traditional Medicine in Africa, Spectrum Books, Ltd, Ibadan, Nigeria, 1993, 157-161.
[8]	HAS, Traitements contre l’hypertension: efficaces, mais souvent trop chers, 2013. Available: https://www.allodocteurs.fr/se-soigner-medicaments-antihypertenseur-traitements-contre-l-hypertension-efficaces-mais-souvent-trop-chers-10353.html [Accessed April 5, 2022]
[9]	OMS, Stratégie de l’OMS pour la Médecine Traditionnelle pour 2014-2023, 2013, 72. Available: https://apps.who.int/iris/bitstream/handle/10665/95009/9789242506099_fre.pdf [Accessed April 5, 2022].
[10]	Chevalier, A., Encyclopédie des plantes médicinales: Identification, préparations, soins, Larousse, Paris, France, 2001, 335.
[11]	Ogbonnia, S.O., Mbaka, G.O., Anyika, E.N., Emordi, J.E. and Nwakakwa, N., “An evaluation of acute and subchronic toxicities of a nigerian polyherbal tea remedy”. Pakistan Journal of Nutrition, 10 (11). 1022-1028. Nov. 2011.
[12]	Ake-Assi, L. and Guinko, S., Plantes utilisées dans la Médecine Traditionnelle en Afrique de l’Ouest, Roche Basel, Switzerland, 1991, 127.
[13]	Kerharo, J. and Adam, J.G., Pharmacopée sénégalaise traditionnelle : Plantes médicinales et toxiques, Ed Vigot et Frères, Paris, France, 1974, 1011.
[14]	Malgras, D., Arbres et arbustes guérisseurs des savanes maliennes. Ed. Karthala et ACCT, Paris, France, 1992, 478.
[15]	Berg, C.C., “Classification and distrubition of Ficus”, Experientia, 45. 605-611. Jan. 1989.
[16]	Ake-Assi, L., “Utilisation des diverses espèces de Ficus (Moraceae) dans la pharmacopée traditionnelle africaine en Côte d’Ivoire. Compte-rendu de la XII Réunion Pléniere de Symposium VIII” in Mitteilungen aus dem Institut für allgemeine Botanik in Hamburg Band, 1990, 1039-1046.
[17]	Berg, C.C. and Wiebes, J.T. African Fig Trees and Fig Wasps, Ed. KNAW, Amsterdam, Niederland, 1998, 208.
[18]	Amonkan, K.A., Konan, B.A., Kouakou, K.L., Bouafou, K.G.M., Bléyéré, N.M., Ahui, M.L.B., Zannou, V.T., Ouattara, H., Datté, J.Y. and Kati-Coulibaly, S., “Criblage phytochimique et effets d’un extrait aqueux de feuilles de Ficus exasperata Vahl. 1805 (Moraceae) sur la pression artérielle et l’activité contractile du cœur chez les mammifères”, Internationnal Journal of Biology and Chemimical Science, 4 (3). 681-691. Jun. 2010.
[19]	Amonkan, K.A., Konan, B.A., Kouakou, K.L., Bléyéré, N.M., Bouafou, K.G.M. and Kati-Coulibaly, S., “Comparative effects of different fractions of crude aqueous extract of Ficus exasperata leaves on blood pressure”, International Current Pharmaceutical Journal, 2 (11). 193-195. Oct. 2013.
[20]	OCDE, “Série sur les principes de bonnes pratiques de laboratoire et vérification du respect de ces principes”, ENV/MC/CHEM, 98. 17. Mar. 1998.
[21]	Europeen Union, “Commission implementing decision of 14 november 2012 establishing a common format the submission of the information pursuant to Directive 2010/63/EU of the Europeen parliament and the council on the protection of animals used for scientific purposes (notified under document C (2012) 8064) text with EEA relevance”, Special Education Croatian, 15 (28). 163-180. Nov. 2012.
[22]	Konan, B.A., Kpahé, Z.F., Koko, K.B. and Adepo, Y.P., “Diuretic activities of root bark aqueous and ethanolic extracts of Parquetina nigrescens. I-Effects on urinary excretion in Wistar rat”, Journal of Drug Delivery and Therapeutics, 12 (3). 57-61. May 2022.
[23]	Amonkan, K.A., Konan, B.A., Bléyéré, N.M., Ahui, M.L.B., Kouakou, K.L., Bouafou, K.G.M. and Kati-Coulibaly, S. “Comparative effects of Ficus exasperata aqueous leaf extract and furosemide on urinary excretion in DOCA-salt hypertensive rat”, Journal of Medical Sciences, 13 (5). 385-390. May 2013.
[24]	Amonkan, K.A., Konan, B.A., Ahui, M.L.B., Bléyéré, N.M., Kouakou, K.L., Bouafou, K.G.M., “Diuretic effects of aqueous extract of Ficus exasperata Vahl. leaves in rat”, Pakistan Journal of Biological Sciences, 16 (21). 1383-1387. Nov. 2013.
[25]	Maghrani, M., Zeggwagh, N., Haloui, M. and Eddouks, M., “Acute diuretic effect of aqueous extract of Retama raetam in normal rats”, Journal of Ethnopharmacology, 99 (1). 31-35. May 2005.
[26]	Adebayo, E.A., Ishola, O., Taiwo, R.O.S., Majolagbe, O.N. and Adekeye, B.T., “Evaluations of the methanol extract of Ficus exasperata stem bark, leaf and root for phytochemical analysis and antimicrobial activities”, African Journal of Plant Science, 3 (12). 283-287. Dec. 2009.
[27]	Lawal, I.O., Borokini, T.I., Oyeleye, A., Williams, O.A. and Olayemi, J., “Evaluation of extract of Ficus exasperata Vahl root bark for antimicrobial activities against some strains of clinical isolates of bacterial and fungi”, International Journal of Modern Botany, 2 (1). 6-12. Aug. 2012.
[28]	Abraham, O.J., Odiba, P.A., Okpanachi, E., Miachi, O.E. and Ocheja, F.B. “Antimicrobial properties of crude ethanolic extract of Ficus exasperata root”. Journal of Medical and Applied Biosciences, 6 (2). 11-20. Jun. 2014.
[29]	Agunu, A., Abdurahman, E.M., Andrew, G.O. and Muhammed, Z., “Diuretic activity of the stem-bark extracts of Steganotaenia araliacea Hochst (Apiacea) “, Journal of Ethnopharmacology, 96 (3). 471-475. Jan. 2005.
[30]	Galati, E.M., Tripodo, M.M., Trovato, A., Miceli, N. and Monforte, M.T., “Biological effect of Opuntia Ficus indica (L.) Mill. (Cactaceae) waste matter. Note I: diuretic activity”, Journal of Ethnopharmacolology, 79 (1). 17-21. Jan. 2002.
[31]	Iloki-Assanga, S.B., Lewis-Luján, L.M., Lara-Espinoza, C.L., Gil-Salido, A.A., Fernandez-Angulo, D., Rubio-Pino, J.L. and Haines, D.D., “Solvents effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum”, BMC Research Notes, 8. 396. Sep. 2015.
[32]	Thouri, A., Chahdoura, H., El Arem, A., Hichri, A.O., Hassin, R.B. and Achour, L. “Effect of solvents extraction on phytochemical components and biological activities of tunisian date seeds (var. Korkobbi and Arechti)”, BMC Complementary and Alternative Medicine, 17. 248. May 2017.
[33]	Dirar, A.I., Alsaadi, D.H.M., Wada, M., Mohamed, M.A., Watanabe, T. and Devkota, H.P., “Effects of extraction solvents on total phenolic and flavonoid contents and biological activities of extracts from Sudanese medicinal plants”. South African Journal of Botany, 120. 261-267. Jan. 2019.
[34]	Onyebuchi, C. and Kavaz, D., “Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts”. Scientific Reports, 10. 21760. Dec. 2020.
[35]	Haas, M. and Forbush, B., “The Na-K-Cl cotransporters”, Journal of Bioenergetics and Biomembranes, 30 (2). 161-172. Apr. 1998.
[36]	Nielsen, S., Maunsbach, A.B., Ecelbarger, C.A. and Knepper, M. A. “Ultrastructural localization of Na-K-2Cl cotransporter in thick ascending limb and macula densa of rat kidney”, American Journal of Physiology, 275 (6). F885-F893. Dec. 1998.
[37]	Odlind, B., Beermann, B., Selen, G. and Persson, A.E., “Renal tubular secretion of piretanide and its effects on electrolyte reabsorption and tubuloglomerular feedback mechanism”, Journal of Pharmacology and Experimental Therapeutics, 225 (3). 742-746. Jun. 1983.
[38]	Koopmans, P.P., Thien, T. and Gribnau, F.W., “Influence of non-steroidal anti-inflammatory drugs on diuretic treatment of mild to moderate essential hypertension”, British Medical Journal (Clinical Research Ed), 289 (6457). 1492-1494. Dec. 1984.
[39]	Lijnen, P., Petrov, V., Tjandramaga, T., Verbesselt, R. and Amery, A. “Erythrocyte and platelet cationic concentrations and transport systems in normal volunteers treated with carmoxirole”, Methods Findings in Experimental and Clinical Pharmacology, 15 (7). 483-490. Sep. 1993.