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American Journal of Public Health Research. 2025, 13(3), 117-132
DOI: 10.12691/ajphr-13-3-3
Open AccessArticle

Investigating the Protective Roles of Mulberry (Morus alba L.) Leaves in Alleviating Benzo[a]pyrene-Induced Liver Damage in Rats

Yousif A. Elhassaneen1, , Sobhi I. Hassab El-Nabi2, Nazeha A. Khalil1 and Sara A. Abd ElMaksoud1

1Department of Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt

2Biology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt

Pub. Date: May 12, 2025
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Cite this paper:
Yousif A. Elhassaneen, Sobhi I. Hassab El-Nabi, Nazeha A. Khalil and Sara A. Abd ElMaksoud. Investigating the Protective Roles of Mulberry (Morus alba L.) Leaves in Alleviating Benzo[a]pyrene-Induced Liver Damage in Rats. American Journal of Public Health Research. 2025; 13(3):117-132. doi: 10.12691/ajphr-13-3-3

Abstract

This study aimed to evaluate the potential protective effects of Mulberry (Morus alba L.) leaf powder (MLP) against benzo[a]pyrene-induced liver damage in rats. MLP was prepared, and its chemical composition, bioactive compounds, and antioxidant properties were analyzed. The results showed that MLP is rich in protein, ash, crude fiber, dietary fiber, carbohydrates, and non-nutrient antioxidants, such as phenolics, carotenoids, flavonoids, polysaccharides, tannins, and kampferol. It also demonstrated strong antioxidant activity, with values of 63.72%, 80.04%, and 82.66% in aqueous, methanol, and ethanol extracts, respectively. For the biological studies, rats were fed MLP at concentrations of 5%, 10%, and 15% (g/100 g) in their basal diet for four weeks following B[a]P-induced hepatotoxicity. Rats exposed to B[a]P showed a significant (p≤0.05) reduction in glycogen content (-66.5%) and glucose-6-phosphate dehydrogenase (G6PD) activity (-60%), while glucose-6-phosphatase (G6Pase) activity increased substantially by 279.7% compared to the normal control group. Additionally, B[a]P exposure resulted in a significant (p≤0.05) increase in the activity of drug-metabolizing enzymes (cytochrome P450, CYP450, 64.6%) and reactive oxygen species (ROS, 267.2%), alongside a significant (p≤0.01) decrease in serum triglycerides (TGs), high-density lipoprotein cholesterol (HDL-c), and glutathione (GSH) levels, with reductions of -15.6%, -37.3%, and -58.4%, respectively, compared to the normal control group. In contrast, the inclusion of MLP in the rat diets for 28 days resulted in significant improvements in liver function, antioxidant status, and lipid metabolism, along with positive changes in liver histopathology. In conclusion, these results suggest that MLP may have potential as a hepatoprotective agent for mitigating liver damage.

Keywords:
Chemical composition bioactive compounds liver functions CYP450 serum lipid profile glutathione

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Voet, D., & Voet, G. (1990). Biochemistry, 1st Edition, John Wiley and Sons, USA.
 
[2]  Elhassaneen, Y. A. (1996). Biochemical and technological studies on pollution of fish with pesticides and polycyclic aromatic hydrocarbons. Ph.D. Thesis., Faculty of Agriculture, Mansoura University, Egypt.
 
[3]  Crawford, J. M. (1999). "Liver and biliary system." In Basic Histopathology, 4th ed., W.B. Saunders, pp. 517-530.
 
[4]  Crawford, J. M. (1999). The Liver and the Biliary Tract., Pathologic Basis of Disease. Eds. R. S. Cotran, V. Kumar, and T. Collins. W. B. Saunders Company: Philadelphia, USA.
 
[5]  Lawrence, R., & Emmet, J. (2012). "The liver: physiology, disease, and pharmacology." Oxford University Press, pp. 100-110.
 
[6]  Lawrence, S. F. & Emmet B. K. (2012). Handbook of Liver Disease, 3rd ed. Elsevier Saunders, Philadelphia, PA.
 
[7]  Lorenz, G. (1985). Liver damage caused by pesticides. Zentralblatt für Allgemeine Pathologie und Pathologische Anatomie, 130(6), 533-538.
 
[8]  Lorenz, S. (1985). The effect of pesticides and aflatoxins on liver function in Egypt. International Journal of Environmental Health, 15(3), 211-219.
 
[9]  Elhassaneen, Y. (2004). The effect of charcoal-broiled meat consumption on the antioxidant defense system of erythrocytes and antioxidant vitamins in plasma. Nutrition Research, 24(6), 435-446.
 
[10]  Elhassaneen, Y. A., Ragab, S., & Badawy, R. (2016). Antioxidant activity of methanol extracts from various plant parts and their potential roles in protecting liver disorders induced by benzo(a)pyrene. Public Health International, 2(1), 38-50.
 
[11]  Elhassaneen, Y., El-Waseef, S., Fathy, N., & Ahmed, S. S. (2016). Bioactive compounds and antioxidant potential of food industry by-products in Egypt. American Journal of Food and Nutrition, 4(1), 1-7.
 
[12]  Elhassaneen, Y., Gorge, S., Elkhamisy, A., & Negm, S. (2016). Removal techniques of aflatoxin from contaminated commodities: Review study. 4th International-18th Arab Conference of Home Economics "Home Economics and Development Issues", 5-6 April 2016, Faculty of Home Economics, Minoufiya University, Egypt. Journal of Home Economics (Special Issue), 26(2), 23-41.
 
[13]  Jørs, E., El-Bayoumy, K., & El-Serag, H. B. (2018). "Environmental and dietary risk factors for liver cancer in Egypt." International Journal of Environmental Research and Public Health, 15(10), 2142-2156.
 
[14]  Jørs, E.; Neupane,D. & London, L. (2018). Pesticide poisonings in low- and middle-income countries, Environmental Health Insights, 12: 1-3.
 
[15]  Anwar, W., Khaled Hussein, M., Amra Hassan, A., El-Nezami, H., & Loffred, C. A. (2008). Changing pattern of hepatocellular carcinoma (HCC) and its risk factors in Egypt: Possibilities for prevention. Mutagenesis, 659, 176-184.
 
[16]  Kenko, N. D.; Fai, P.; Ngameni, T. N. & Mbida, M. (2017). Environmental and human health assessment in relation to pesticide use by local farmers and the Cameroon Development Corporation (CDC), Fako Division, South-West Cameroon,” European Scientific Journal, 13, ( 21): 454–473.
 
[17]  Andrade, Z.A. (2009).Schistosomiasis and liver fibrosis. Parasite Immunol. 31(11):656-63.
 
[18]  Mohammad, M., Abdel-Aziz, F., Elhassaneen, M. A., Ahmed, M., & Farouk, H. (2020). [Further information needed to complete the citation].
 
[19]  WHO (2010). Global Surveillance, Control of Hepatitis C. Report of a WHO Consultation organized in collaboration with the Viral Hepatitis Prevention Board, Antwerp, Belgium. J Viral Hepat, 6:35–47.
 
[20]  World Health Organization (WHO). (2010). "Global burden of hepatitis and liver cancer." World Health Organization Report, Geneva, Switzerland.
 
[21]  El-Zayadi, A.R. (2004). Curse of schistosomiasis on Egyptian liver. World J Gastroenterol. 15; 10(8): 1079-81.
 
[22]  Tomah S, Hamdy O, Abuelmagd MM, Hassan AH, Alkhouri N, Al-Badri MR, Gardner H, Eldib AH, Eid EA. (2021). Prevalence of and risk factors for non-alcoholic fatty liver disease (NAFLD) and fibrosis among young adults in Egypt. BMJ Open Gastroenterol. 2021 Oct; 8(1): e000780.
 
[23]  Gray, J. I., & Morton, D. I. (1981). Some toxic compounds produced in food by cooking and processing: A review. Journal of Human Nutrition, 35, 5-23.
 
[24]  Elhassaneen, A., & Tawfik, L. (1998). The presence of some carcinogens in human foods distributed in Egyptian local markets. Journal of Home Economics, 8(3), 23-38.
 
[25]  Elhassaneen Y., & El-Badawy, A. (2013). Influence of charcoal broiled meat consumption on the liver functions and non-enzymatic antioxidants in human blood. Food and Nutrition Sciences, 4(1), 90-99.
 
[26]  Emerole, G. O., Uwaifo, A. O. & Bababunmi, E.A. (1982): The presence of aflatoxine and some polycyclic aromatic hydrocarbons in human foods. Cancer Lett. , 15: 123-129.
 
[27]  Larsson, A., Orrenius, S., Holmgren, A. and Mannervik, B. (1983): Eds., Functions of glutathione, Raven Press, New York.
 
[28]  Van Maanen, J.M., Moonen, E.J., Maas, L.M., Kleinjans, ‎J.C. & Schooten van, F.J. (1994): Formation of aromatic ‎DNA adducts in white blood cells in relation to urinary ‎excretion of 1-hydroxypyrene during consumption of grilled ‎meat. Carcinogenesis, 15: 2263-2268.‎
 
[29]  Bassiouny, E. (1999). The effect of grilled meat consumption on health“M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Menoufia University, Egypt.
 
[30]  U.S. Environmental Protection Agency. (2005). Supplemental guidance for assessing cancer susceptibility from early-life exposure to carcinogens.
 
[31]  Harvey, R. G. (1985). Polycyclic hydrocarbons and carcinogenesis. ACS Symposium Series, 283, American Chemical Society, Washington, D.C.
 
[32]  Plakunov, I., Smolarek, T.A., Fischer, L.D., Wiley, J.C., & ‎Baird, W.M. (1987): Separation by ion-pair high-performance ‎liquid chromatography of the glucuronid, sulfate and ‎glutathione conjugates formed from benzo(a)pyrene in cell ‎cultures rodents, fish and humans. Carcinogenesis, 8: 59 – 66.‎
 
[33]  Hawkins, E. W., Walker, W. W., Overstreet, R. M., Lytle, T. F. & Lytle, J. S. (1990). Carcinogenic effects of some polycyclic aromatic hydrocarbons on the Japanese medaka and Guppy in waterborne exposures. The Science of the Total Environment, 94: 155-167.
 
[34]  Elhassaneen, Y. (2002). New and quickly biological method for detection the potential chemical toxins and/or carcinogens in foods. Proceedings of2nd scientific Conference on Foodborne Contamination and Egyptian’s Health (24–24 April), Faculty of Agriculture, Mansoura University, Mansoura, Egypt, pp 371-394.
 
[35]  Kaarthik J., , , Mona, I., Gail, M., Ofelia, A. , Igor, P. & Miriam, C. (2012). Benzo[a]pyrene (BP) DNA adduct formation in DNA repair–deficient p53 haploinsufficient [Xpa(−/−)p53(+/−)] and wild-type mice fed BP and BP plus chlorophyllin for 28 days. , 33(11): 2236–2241.
 
[36]  El-Safty, A. (2012). Production of some important nutritional and functional compounds from the by-products of food processing companies. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[37]  Elhassaneen Y. & Sayed, R. (2015). Polycyclic aromatic hydrocarbons formation in charcoal broiled meatballs are reduced by addition of onion peel extracts to ground beef. Proceeding of 2nd International Conference on Food and Biosystems Engineering (2nd I.C. FABE2015), 28-31 May, 2015), [Vol. II, PP. 95-104], Mykonos, Greece.
 
[38]  Elmaadawy, A., Arafa, R., & Elhassaneen, Y. (2016). Oxidative stress and antioxidant defense systems status in obese rats feeding some selected food processing by-products applied in bread. Journal of Home Economics, 26(1), 1-37.
 
[39]  Aly, A. S., Elbassyouny, G. M., & Elhassaneen, Y. A. (2017). Studies on the antioxidant properties of vegetable processing by-products extract and their roles in alleviating health complications caused by diabetes in rats. Proceedings of the 1st International Conference of the Faculty of Specific Education, Kafrelsheikh University, 1-24, Sharm El Sheikh, Egypt, 24-27 October 2017.
 
[40]  Butt, M. S., Nazir, A., Sultan, M. T., & Schoёn, K. (2008). “Morus alba L. Nature’s Functional Tonic.” Trends in Food Science and Technology 19 (10): 505-12.
 
[41]  Ewa F., Joanna, K., Monica, P., Jozef, K.; Marian, R., Eugeniusz, K. & Maciej, B. (2013). Chemical characterization and antioxidative properties of Polish variety of Morus alba L. leaf aqueous extracts from the laboratory and pilot-scale processes.Agricultural Sciences,4 (5B): 141-147.
 
[42]  Doi, K., Kojima, T., Makino, M., Kimura, Y., & Fujimoto, Y. (2001). Studies on the constituents of the leaves of Morus alba L. Chemical and Pharmaceutical Bulletin, 49, 151-153.
 
[43]  Anastasia-Varvara F. & (2016). Berry Leaves: An Alternative Source of Bioactive Natural Products of Nutritional and Medicinal Value. . 5(2): 17-27.
 
[44]  Harauma, A., Murayama, T., Ikeyama, K., Sano, H., Arai, H., & Takano, R. (2007). Mulberry leaf powder prevents atherosclerosis in apolipoprotein E-deficient mice. Biochemical and Biophysical Research Communications, 358, 751-756.
 
[45]  A.O.A.C. (1995). “Official Methods of the Association of Official analytical Chemists16 th Ed. Published by the Association of Official Analytical Chemists. Arlington, Virginia, VA.
 
[46]  Singleton, V. L. & Rossi, J. A., Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16: 144-158.
 
[47]  Wolfe, K., Wu, X., & Liu, R. H. (2003). Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, 51, 609–614.
 
[48]  Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. In R. Douce & L. Packer (Eds.), Methods in Enzymology (pp. 350-382). Academic Press Inc., New York.
 
[49]  Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559.
 
[50]  Vazirian, M., Dianat, S., Manayi, A., Ziari, R., Mousazadeh, A., Emran, H., Saeidnia, S., & Amanzadeh, Y. (2014). Anti-inflammatory effect, total polysaccharide, total phenolics content, and antioxidant activity of the aqueous extract of three basidiomycetes. Research Journal of Pharmacognosy, 1, 13-19.
 
[51]  Van-Burden, T. P., & Robinson, W. C. (1981). Formation of complexes between protein and tannic acid. Journal of Agricultural and Food Chemistry, 1, 77.
 
[52]  Kumari. T., S. Singh, & A. D. Tiwari (2016). "Colorimetric Determination of Kaempferol Using Aluminum Chloride Reagent." Analytical Biochemistry, 508, 42-48.
 
[53]  Porra, R. J., Thompson, W. A., & Kriedemann, P. E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 975(3), 384-394.
 
[54]  Marco, G. J. (1968). A rapid method for evaluation of antioxidants. J. Am. Oil Chem. Soc., 45: 594-598.
 
[55]  Reeves, P., Nielsen, F. & Fahey, G. (1993) AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. Journal of Nutrition 123(11): 1939-1951.
 
[56]  NRC, National Research Council (1996) Guide for the Care and Use of Laboratory Animals Washington: National Academy Press.
 
[57]  Hasegawa, R; Chujo, T; Sai-kato, K.; Umemura, T; Tanimura, A. and Kurokawa, Y (1995). Preventive Effects of Green Tea Against Liver Oxidative DNA Damage and Hepatotoxicity in Rats Treated with 2-Nitropropane, Fd Chem. Toxic. 33 (11): 961-970.
 
[58]  Chapman, D.G., Castilla, R. & Champbell, J.A. (1959). Evaluation of protein in food. I.A. Method for the determination of protein efficiency ratio. Can. J. Biochemistry Physiology, 37(5):679-86. PMID: 13651969.
 
[59]  Drury, R.A. & Wallington, E.A. (1980). Carlton's Histological Technique. 5th ed. Oxford University.
 
[60]  El-Khawaga, O. Y., Abou-Seif M.A., El-Waseef A. & Negm A.A. (2010). Hypoglycemic, Hypolipidemic and Antioxidant Activities of Cleome droserifolia in Streptozotocin-Diabetic Rats. Journal of Stress Physiology & Biochemistry, 6(4). 28-41.
 
[61]  Damsbo, P., Vaag, A., Hother-Nielsen, O., & Beck-Nielsen, H. (1991). Reduced glycogen synthase activity in skeletal muscle from obese patients with and without type 2 diabetes mellitus. Diabetologia, 34(4), 239-245.
 
[62]  Chan, T.K., Todd, D. & Wong, C.C. (1965). Tissue levels in erythrocyte glucose-6-phosphate dehydrogenase deficiency. J Lab Clin Med, 6: 936-940.
 
[63]  Rossetti, L., Lee, Y. T., Ruiz, J., Aldridge, S., Shamoon, H., & Boden, G. (1993). Quantitation of glycolysis and skeletal muscle glycogen synthesis in humans. American Journal of Physiology, 295, 761-769.
 
[64]  Ahmadi, S. A., Boroumand, M., Gohari-Moghaddam, K., Tajik, P., & Dibaj, S. (2008). The impact of low serum triglyceride on LDL-cholesterol estimation. Archives of Iranian Medicine, 11, 318-321.
 
[65]  Fossati, P., & Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry, 28(10), 2077-2080.
 
[66]  Ellman, G. L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82(1), 70-77.
 
[67]  Buege, J. A., & Aust, S. D. (1978). Microsomal lipid peroxidation. In L. Packer (Ed.), Methods in Enzymology (Vol. 52, pp. 302-310). New York, NY: Academic Press.
 
[68]  Omura, T., & Sato, R. (1964). The carbon monoxide difference spectrum of liver microsomes. Journal of Biological Chemistry, 239(7), 2370-2378.
 
[69]  Bancroft, J. D., Stevens, A., & Turner, D. R. (1996). Theory and Practice of Histological Techniques (4th ed.). Churchill Livingstone.
 
[70]  Chaudhary, R., Yadav, S. K., & Singh, M. (2017). Moisture content and preservation of dried plant materials. Journal of Agricultural Science, 8(2), 123-130.
 
[71]  Jafari, S. M., Ehsani, M. R., & Fathi, M. (2020). Study of the chemical composition and nutritional profile of spinach. Food Chemistry, 312, 126052.
 
[72]  Bhattacharya, A., Saha, P., & Roy, D. (2019). Comparative study of the chemical composition of Amaranthus leaves. Journal of Food Science and Technology, 56(3), 1399-1407.
 
[73]  Bhat, A. H., Sharma, N., & Pandey, R. (2018). Nutritional and functional properties of spinach leaves. Journal of Food Science and Technology, 55(2), 652-659.
 
[74]  Akhtar, M. S., Ahmed, S. M., & Khan, A. M. (2017). Nutritional and functional properties of Moringa (Moringa oleifera) leaves. Food Research International, 101, 128-137.
 
[75]  Akinmoladun, O. F., Adeyemi, O. S., & Olatunji, G. A. (2018). Nutritional and phytochemical composition of Moringa oleifera leaves. Food Science & Nutrition, 6(5), 1570-1576.
 
[76]  Sulaiman, N., Hassan, M. A., & Ibrahim, M. (2020). The effect of geographical origin on the nutritional composition of mulberry leaves. Journal of Agricultural and Food Chemistry, 68(15), 4439-4447.
 
[77]  Hekal, H. A. A. (2012). Nutritional, biochemical, and microbiological studies on mulberry [M.Sc. Thesis, Faculty of Home Economics, Minoufiya University, Egypt].
 
[78]  Abd El-Mageed, L. S. M. (2012). Magnification of Using Berries (Leaves and Fruits) on Food in Food Technology and Therapeutic Nutrition. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[79]  El-Nassag, D. E., Ghamry, H. I., & Elhassaneen, Y. A. (2019). Stevia (Stevia rebaudiana) leaves: Chemical composition, bioactive compounds, antioxidant activities, antihyperglycemic and antiatherogenic effects. Journal of Studies and Searches of Specific Education, 5(1), 157-180. [http:// www.jse.zu.edu.eg/ index.php/jse/article/view/97/96].
 
[80]  Elhassaneen, Y. A., Nour El-Deen, A. A., & Nasef, A. Z. (2023). Ultraviolet-C Radiation Induced Changes on Bioactive Compounds Content, Antioxidant Capacity, and Microbial Quality of Minimally Processed Molokhia (Corchorus Olitorius L.) Leaves. Journal of Agriculture and Crops, 9(3), 309-322.
 
[81]  Elhassaneen, Y. A., El-Bassouny, G. M., Emam, O. A., & Ammar, H. E. (2024-a). Strawberry and Cauliflower Leaves Are Rich in Bioactive Compounds and Antioxidant Activity: Application on Obese Rats. American Journal of Public Health Research, 12(4), 64-80.
 
[82]  Elhassaneen, Y. A., Gharib, M. A., & Omara, A. Z. (2024-b). Enrichment of Egyptian Balady Bread with Nutrients and Bioactive Compounds through Mixing It with Sweet Potato and Cauliflower Leaves Powder. American Journal of Food Science and Technology, 12(6), 192-206.
 
[83]  Kumari, N., Prasad, R., & Kumar, A. (2019). Proximate composition and phytochemical evaluation of mulberry leaves. Journal of Food Science & Technology, 56(6), 3034-3043.
 
[84]  Patel, S., Patel, M., & Bhutani, M. (2018). Health benefits of dietary fibers from plant sources. International Journal of Food Sciences, 12(2), 118-127.
 
[85]  El-Barbary, A. K. M. Z. (2019). Evaluation of bioactive compounds of stevia (Stevia rebaudiana) leaves and their antihyperglycemic effects in alloxan-induced diabetic rats. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[86]  Khoneem, A. (2009). Antioxidant activity of some vegetables, spices, and herbs distributed in Egyptian local markets. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[87]  Jaggi, L. (2012). Turmeric, Curcumin and Our Life: A Review. Bull. Environ. Pharmacol. Life Sci.; 1 (7): 11 – 17.
 
[88]  Elhassaneen, Y., Sherif, R., Alaa, E., & Emad, A. (2013). Mango peel powder: A potential source of phenolics, carotenoids, and dietary fiber in biscuit preparations. 2nd International-16th Arab Conference of Home Economics "Home Economics in the Service of Industry", 10-11 September 2013, Faculty of Home Economics, Minoufiya University, Egypt. Journal of Home Economics (Special Issue), 23(4), 1-16.
 
[89]  Chaitanya, K. V., Pathan, A. A. K., Mazumdar, S. S., Charavarthi, G. P., Parine, N., & Bobbarala, V. (2010). Role of oxidative stress in human health: An overview. Journal of Pharmacy Research, 3, 1330-1333.
 
[90]  El-Harbi, E. N. (2018). Nutritional and technological studies on some plant parts and their effects on obesity complications induced in experimental animals. Faculty of Specific Education, Benha University, Benha, Egypt
 
[91]  Sayed Ahmed, S. (2016). Nutritional and technological studies on the effect of phytochemicals on obesity injuries and their related diseases by using experimental animals. Ph.D. Thesis in Home Economics (Nutrition and Food Science), Faculty of Specific Education, Port Said University, Egypt.
 
[92]  Elbasouny, G., Shehata, N., & Elhassaneen, Y. A. (2019). Feeding of some selected food industries by-products induced changes in oxidants/antioxidant status, lipids profile, glucose, and immunological parameters of blood obese rats. The 6th Scientific and 4th International Conference "The Future of Specific Education and People with Special Needs in Light of the Concept of Quality", 24-26 February 2019, Faculty of Specific Education, Ain Sokhna University, El-Ain El-Soghna, Egypt.
 
[93]  Mater, E. N. A. (2019). Effect of Papaya (Leaves and Seeds) on Rat Liver Disorders Induced by Carbon Tetrachloride. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[94]  Darwish, A. H. A. (2020). Potential Therapeutic Applications of Persimmon (Diospyros kaki-Virginiana) Fruits and Leaves as Evaluated on Diabetic and Hepatopathic Male Albino Rats. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[95]  Abd Elalal, N., El Seedy, G. & Elhassaneen, Y. (2021). Chemical Composition, Nutritional Value, Bioactive Compounds Content and Biological Activities of the Brown Alga (Sargassum Subrepandum) Collected from the Mediterranean Sea, Egypt. Alexandria Science Exchange Journal, vol. 42, (4), pp. 893-906.
 
[96]  Abd El-rahman, A. N. (2021). Effects of some Egyptian plant herbs on hypercholestermic rats treated with carbon Tetrachloride" Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[97]  Badawy, E. Z. M. (2022). Study on the effect of strawberry leaves on diabetic rats induced by alloxan. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
 
[98]  Elhassaneen, Y. A., Nasef, A. Z., & Abdel Rhman, N. (2021-a). Potential Effects of Olive and Mango Leaves on Alloxan Induced Diabetes Complications in Rats. Journal of Home Economics, 31(2), 49-62.
 
[99]  Elhassaneen, Y. A., Abd El-Rahman, A. N., & El-Samouny, S. H. (2021-b). Potential Protective Effects of Cauliflower Leaves and Prickly Pear Fruits Skin on Liver Disorders Induced by Carbon Tetrachloride in Rats. Journal of Home Economics, 32(1), 19-42.
 
[100]  Elhassaneen, Y. A ., Hassab El-Nabi, S. E., Mahran, M. Z., Bayomi, A. I. & Badwy, E. Z. (2022). Potential Protective Effects of Strawberry (Fragaria Ananassa) Leaves Against Alloxan Induced Type 2 Diabetes in Rats: Molecular, Biological and Biochemical Studies. Sumerianz Journal of Biotechnology, 5(1): 1-15.
 
[101]  Elhassaneen, Y. A., Emam, O., El-Bassouny, G., & El-Qalaaf, G. (2022). Effect of cabbage and radish leaves on obesity biological changes induced in rats. Journal of the College of Specific Education for Educational and Specific Studies, 7(19), 1-33.
 
[102]  EIhassaneen Y. A., Afifi, T. A., Elhefny, M. A., & Bayomi, A. I. (2024). Effect of Silybum marianum seeds extract intervention on biochemical parameters, histological changes, and apoptosis and cell cycle of liver tissue in benzo[a]pyrene-injected rats. American Journal of Food and Nutrition, 12(1), 1-15.
 
[103]  Elhassaneen, Y. A., El-Bassouny, G. M., Emam, O. A., & Ammar, H. E. (2024). Strawberry and cauliflower leaves are rich in bioactive compounds and antioxidant activity: Application on obese rats. American Journal of Public Health Research, 12(4), 64-80.
 
[104]  Elhassaneen, Y. A., ElBassouny, G. M., Emam, O. A., & Aram, E. I. (2024). Nutrients and nutraceuticals content and in vitro biological activities of formulae from plant parts commonly spread in Egyptian markets. American Journal of Food and Nutrition, 12(5), 134-151.
 
[105]  Patel, S. S., Srivastava, M., & Mishra, A. (2015). Hepatoprotective and antioxidant properties of Morus alba L. leaves: A review. Journal of Medicinal Plants Research, 9(7), 140-147.
 
[106]  Singh, S., Yadav, S., & Sharma, R. (2017). The antioxidant and anti-inflammatory effects of Morus alba in liver disorders. Antioxidants, 6(3), 52.
 
[107]  Fang, L., Li, Z., & Zhang, Y. (2019). Flavonoids from Morus alba as a therapeutic agent in oxidative stress-related diseases. Antioxidants, 8(10), 494-505.
 
[108]  Fang, M., Wang, L., & Zhang, H. (2019). Dose-dependent effects of plant-derived hepatoprotective agents in experimental liver injury models. Phytotherapy Research, 33(4), 878-890.
 
[109]  El-Banaa, S. (2014). Phytochemicals in Artichoke and their effects on liver cancer induced by carbon tetrachloride, CCl4" M.Sc. Thesis in Nutrition and Food Science, Faculty of Home, Minoufiya University, Egypt.
 
[110]  Mansour, Z. M. A. (2017). Hepatoprotective activity and antioxidant effects of avocado (Persea americana) fruits on rat's hepatotoxicity induced by carbon tetrachloride. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[111]  Tahoon, S. R. (2019). Hepatoprotective effect of apricot and plum kernel on carbon tetrachloride induced hepatic rats. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[112]  Sayed-Ahmed, S.; Shehata, V. & Elhassaneen, Y. (2020). Potential Protective Effects of Ganoderma lucidum Powder against Carbon Tetrachloride Induced Liver Disorders in rats: Biological, Biochemical and Immunological Studies, Egypt. Bulletin of the National Nutrition Institute of the Arab Republic of Egypt, 56(2): 99-132.
 
[113]  Elhassaneen, Y., Elkamisy, A., Sayed, R., & Hamza, R. (2021). The bioactive compounds content and antioxidant activities of some plant parts formulae distributed in Egyptian local markets. Port Said Specific Research Journal (PSSRJ), 14(2), 585-608.
 
[114]  Mahran, M. Z., & Elhassaneen, Y. A. (2023). Attenuation of benzo[a]pyrene-induced oxidative stress and cell apoptosis in albino rats by wild milk thistle (Silybum marianum L.) seeds extract. Egyptian Journal of Chemistry, 66 (SI: 13), 1671-1687.
 
[115]  Elhassaneen, Y. A., Garib, M. A., Bayomi, A. I., & Salama, E. E. (2025). Benzo[a]pyrene induced toxicity and molecular damage in liver of rats: Possible protective effects of onion (Allium cepa) skin extract. American Journal of Food and Nutrition, 13(1), 32-47.
 
[116]  Fayez, F. (2016). The effect of turmeric and curcumin on liver cancer induced by benzo[a]pyrene in rats [M.Sc. Thesis, Faculty of Specific Education, Port Said University, Port Said, Egypt].
 
[117]  Mahran, M. Z., Elbassyouny, G. M., & Elhassaneen, Y. (2018). Preventive effects of onion skin powder against hepatotoxicity in rats treated with benzo(a)pyrene. Proceedings of the Annual Conference (13th Arab; 10th International), 11-12 April, Faculty of Specific Education, Mansoura University, "Higher Education in Egypt and the Arab World in the Light of Sustainable Development Strategies," Mansoura, Egypt.
 
[118]  Elhassaneen, Y. A., El-Kholie, E. M., Zaki, A. N., & Yassen, H. A. (2018). Potential effects of cauliflower leaves powder on obese rats. Proceedings of the Annual Conference (13th Arab; 10th International), 11-12 April, Faculty of Specific Education, Mansoura University, "Higher Education in Egypt and the Arab World in the Light of Sustainable Development Strategies", Mansoura, Egypt. [http:// sefac.mans.edu.eg/ english/ mokatamar.htm].
 
[119]  Elhassaneen, Y., Ghamry, H. & Lotfy, L. (2018). Potential chemoprevention of liver disorders by dietary curcumin in rats treated with benzo(a)pyrene. Proceeding of the 1st Scientific International Conference of the Faculty of Specific Education, Minia University, “Specific Education, innovation and labor market” 16-17 Juli, 2018, Minia, Egypt.
 
[120]  Ajami, A. M. (2022). Study the effects of Turmeric on Liver disorder induced by Benzo(a)pyrene. M.Sc. Thesis in Nutrition and Food Science, Faculty of Specific Education, Benha University, Benha, Egypt.
 
[121]  Elhassaneen, Y.A & Mahrran, M.Z. (2024). Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological Studies.
 
[122]  Hassan, R.A., F.M. Shehab El-Din & Y.A. Elhassaneen (1996). Interactive effects of benzo(a)pyrene and xenobiotic chemicals on benzo(a)pyrene carcinogenicity of liver Spot (Leiostomus xanthurus) hepatocytes. Procedding of the Conference on Food Borne Contamination & Egyptian's Health (Nov. 26-27) pp. 249-259, Faculty of Agriculture, University of Mansoura, Mansoura, Egypt. [ISSN 1110-0346].
 
[123]  Elhassaneen, Y. A., Khater, O. M., & Morsey, A. A. (1997). The effect of vitamins on the cancer initiation induced by some food chemical carcinogenic pollutants in vitro. Proceeding of the 2nd Conference: The Role of Women and Egyptian Associations in Environment Protection and Community Development, 25-26 August, Dept. of Home Economics, Faculty of Agriculture, Alexandria University, Egypt, pp. 257-282.
 
[124]  Elhassaneen, Y. (2000). Polycyclic aromatic hydrocarbons in foods: sources, occurrence, toxicological effects and therapy. Proceeding of the First Mansoura Conference of Food Science and Dairy Technology (17 – 19 October), Faculty of Agriculture, Mansoura University, Mansoura, Egypt. pp 319-341. [ISSN 1110-0346].
 
[125]  Elhassaneen, Y. A. (1999).Toxicological and biochemical effects of polycyclic aromatic hydrocarbon compounds produced in fish by cooking and processing. 6 th Arabic Conference on Food Science and Technology, 16 – 18 th march, Egyptian Society of Food Science and Technology, Cairo, Egypt 249 – 270.
 
[126]  Elhassaneen Y. A. (2000). A new biological model system for the determination of industrial and agricultural effluents polluted the aquatic environment in vitro. Pakistan J. of Biological Science, 3 (3): 378-382.
 
[127]  Badawy, R. M. (2017). The effect of phytochemical extracts of some plant parts in liver cancer initiation induced by benzo(a)pyrene. Ph.D. Thesis in Nutrition and Farchood Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[128]  Gao, H., Zhang, L., Chen, X., & Wang, X. (2020). Regulation of glucose-6-phosphatase activity and its implications for liver metabolism. Journal of Hepatology, 72(4), 712-725.
 
[129]  Badawi, A. R. (2023). The effect of reishi mushroom (canoderma lucidum) on liver cancer induced by benzo[a]pyrene in rats. M.Sc. Thesis in Nutrition and Food Science, Faculty of Specific Education, Port Said University, Port Said, Egypt.
 
[130]  El-Nagar, M. A. E. (2024). Biochemical and molecular studies on the effect of milk thistle (Silybum marianum) on benzopyrene-induced hepatotoxicity in rats [MSc. Thesis, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt].
 
[131]  Badawy, F. E. E. (2008). The influence of some chemical, physical, and industrial treatments on the antioxidant activity of onion. M.Sc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
 
[132]  Poma, P., Villa, F. & De Giori, G. (2011). Antioxidant properties of Morus alba and their relevance in liver diseases. Phytotherapy Research, 25(2), 204-212.
 
[133]  Elhassaneen, Y. A., Sabry, S., Musalum, T., El-Eskafy, A., & Abd El-Fatah, A. (2012). Effect of Sweet Violet (Viola odorata L.) blossoms powder on liver and kidney functions as well as serum lipid peroxidation of rats treated with carbon tetrachloride. The Journal of American Science, 9(5), 88-95. [ISSN 1545-1003].
 
[134]  Elhassaneen, Y. A., Nasef, A. Z., Arafa, R. S., & Bayomi, A. I. (2023). Bioactive compounds and antioxidant activities of milk thistle (Silybum marianum) extract and their potential roles in the prevention of diet-induced obesity complications. American Journal of Food Science and Technology, 11(3), 70-85.
 
[135]  Kaneko, T., Hishida, A., & Kinoshita, M. (1994). Oxidation of low-density lipoprotein and damage to endothelial cells in the early development of atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 14(10), 1683-1690.
 
[136]  Aviram, M., Dornfeld, L., Rosenblat, M., & Levy, A. P. (1999). Reduction of low-density lipoprotein oxidation and its consequences for atherosclerosis by antioxidants and other natural compounds. Free Radical Biology and Medicine, 27(4-5), 804-810.
 
[137]  Boraey, R.A.F. (2023). Study of The Biological Activities of Ashwagandha Extract and Its Effects on Neurological Complications in Obese. MSc. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt (December, 2023).
 
[138]  Halliwell, B., & Gutteridge, J. M. C. (1985). Free radicals in biology and medicine (1st ed.). Clarendon Press., USA.
 
[139]  Abd Elalal, N., Elsemelawy, S. & Elhassaneen, Y. (2022). Potential Effects of Wild Milk Thistle (Silybum marianum L.) Seed Extract Intervention on Oxidative Stress Induced by Busulfan Drug in Different Organs of Rats. International Journal of Healthcare and Medical Sciences, vol. 8(3), pp.19-34.
 
[140]  Elhassaneen, Y. A., ElBassouny, G., Moharem, E., & Ahmed, B. (2022). Preservation of leafy vegetables by co-treatment with refrigeration process and ultraviolet radiation (UV-C) and its potential effects on bioactive compounds content and antioxidant activity. Journal of the College of Specific Education for Educational and Specific Studies (SJSE), 7(22), 584-615.
 
[141]  Elhassaneen, Y. A., Emam, O., El-Bassouny, G., & El-Qalaaf, G. (2022). Effect of Cabbage and Radish Leaves on Obesity Biological Changes Induced in Rats. Journal of the College of Specific Education for Educational and Elhassaneen Specific Studies, 7(19), 1-33.
 
[142]  Faisal, M., Elhassaneen, Y.A., Adham, K. & Hassan, R. (1995). Formation of DNA adducts and activation of xenobiotics by hepatocytes of Spot (Leiostomus xanthurus) in vitro. Fish Health Section of the American Fisheries Society and the and the Eastern Fish Disease Workshop, (July 19-22), Syracuse, New York, USA (Abstract).
 
[143]  Abenavoli, L., Milic, N., & Gallo, A. (2020). Polycyclic aromatic hydrocarbons and liver injury: The role of cytochrome P450 enzymes in benzo[a]pyrene metabolism. Journal of Clinical Medicine, 9(5), 1397.
 
[144]  Rangi, S., Dhatwalia, S. K., Bhardwaj, P., Kumar, M., & Dhawan, D. K. (2018). Evidence of similar protective effects afforded by white tea and its active component 'EGCG' on oxidative-stress mediated hepatic dysfunction during benzo(a)pyrene induced toxicity. Food and Chemical Toxicology, 116(Pt B), 281-291.
 
[145]  Hajam, Y. A., Rani, R., Ganie, S. Y., Sheikh, T. A., Javaid, D., Qadri, S. S., Pramodh, S., Alsulimani, A., Alkhanani, M. F., Harakeh, S., Hussain, A., Haque, S., & Reshi, M. S. (2022). Oxidative stress in human pathology and aging: Molecular mechanisms and perspectives. Cells, 11, 552.
 
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