Enterotoxin Detection by PCR in Klebsiella pneumoniae Isolated from Diarrheal Cases among Children in Kirkuk City - Iraq

Siham Sh. AL-Salihi¹, Ali S. Al-Jubouri², Asa'ad F. Albayati^3, and Yusra AR. Mahmood⁴

¹Medical lab, Department, Technical College, Kirkuk, Iraq

²Department of Life Science, Al-Iraqia University, Baghdad, Iraq

³Department of Medical Microbiology, College of medicine, Al-Iraqia University, Baghdad, Iraq

⁴Department of Pediatrics, College of medicine, Al-Iraqia University, Baghdad, Iraq

Cite this paper:
Siham Sh. AL-Salihi, Ali S. Al-Jubouri, Asa'ad F. Albayati and Yusra AR. Mahmood. Enterotoxin Detection by PCR in Klebsiella pneumoniae Isolated from Diarrheal Cases among Children in Kirkuk City - Iraq. American Journal of Medical Sciences and Medicine. 2016; 4(5):92-96. doi: 10.12691/ajmsm-4-5-1

Abstract

The presence of genes encoding the heat stable (ST) and heat labile (LT) enterotoxins were detected in Klebsiella spp. using the PCR method with pairs of specific primers designed for the sta, stb, and lth genes described for diarrheagenic Escherichia coli. It was found that the plasmid DNA of the 8 (40%) of the 20 Klebsiella pneumoniae isolates isolated from diarrhea cases in children contained the enterotoxin-encoding genes (sta, stb, lth), sta gene was the most frequently in the strains 7 isolates with (35%), stb genes 2 isolates with (10%) and lth 1 isolates with (5%). Of which (1) K. pneumoniae isolate had plasmid DNA included all three genes and 12 did not have any of the genes. None of the strains of K. oxytoca, K. terrigena and K. ornithinolytica, isolated from children with diarrhea, had genes encoding the tested enterotoxins.

Keywords:
Klebsiella pneumonia Enterotoxin sta Kirkuk

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