Article citationsMore >>

Alessio M, Albano F, Tarallo F, Guarino A, “Interspecific plasmid transfer and modification of heat-stable enterotoxin expression by Klebsiella pneumoniae from infants with diarrhea,” Ped Res, 33, 205-208, 1993.

has been cited by the following article:

Article

Enterotoxin Detection by PCR in Klebsiella pneumoniae Isolated from Diarrheal Cases among Children in Kirkuk City - Iraq

1Medical lab, Department, Technical College, Kirkuk, Iraq

2Department of Life Science, Al-Iraqia University, Baghdad, Iraq

3Department of Medical Microbiology, College of medicine, Al-Iraqia University, Baghdad, Iraq

4Department of Pediatrics, College of medicine, Al-Iraqia University, Baghdad, Iraq


American Journal of Medical Sciences and Medicine. 2016, Vol. 4 No. 5, 92-96
DOI: 10.12691/ajmsm-4-5-1
Copyright © 2016 Science and Education Publishing

Cite this paper:
Siham Sh. AL-Salihi, Ali S. Al-Jubouri, Asa'ad F. Albayati, Yusra AR. Mahmood. Enterotoxin Detection by PCR in Klebsiella pneumoniae Isolated from Diarrheal Cases among Children in Kirkuk City - Iraq. American Journal of Medical Sciences and Medicine. 2016; 4(5):92-96. doi: 10.12691/ajmsm-4-5-1.

Correspondence to: Asa'ad  F. Albayati, Department of Medical Microbiology, College of medicine, Al-Iraqia University, Baghdad, Iraq. Email: molarfast@gmail.com

Abstract

The presence of genes encoding the heat stable (ST) and heat labile (LT) enterotoxins were detected in Klebsiella spp. using the PCR method with pairs of specific primers designed for the sta, stb, and lth genes described for diarrheagenic Escherichia coli. It was found that the plasmid DNA of the 8 (40%) of the 20 Klebsiella pneumoniae isolates isolated from diarrhea cases in children contained the enterotoxin-encoding genes (sta, stb, lth), sta gene was the most frequently in the strains 7 isolates with (35%), stb genes 2 isolates with (10%) and lth 1 isolates with (5%). Of which (1) K. pneumoniae isolate had plasmid DNA included all three genes and 12 did not have any of the genes. None of the strains of K. oxytoca, K. terrigena and K. ornithinolytica, isolated from children with diarrhea, had genes encoding the tested enterotoxins.

Keywords