Isolation and Molecular Characterization of Cellulolytic Bacillus Isolates from Soil and Compost

Elhadi A. I. Elkhalil^1, , Fatima Y. Gaffar¹, Marmar A. El Siddig² and Huda A. H. Osman¹

¹Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, 13314 Shambat, Sudan

²Department of Botany, Faculty of Science, University of Khartoum, Sudan

Cite this paper:
Elhadi A. I. Elkhalil, Fatima Y. Gaffar, Marmar A. El Siddig and Huda A. H. Osman. Isolation and Molecular Characterization of Cellulolytic Bacillus Isolates from Soil and Compost. American Journal of Microbiological Research. 2015; 3(2):55-58. doi: 10.12691/ajmr-3-2-2

Abstract

Fifty five Bacillus isolates were isolated from compost, and alkaline silty clay soil (rhizosphere of potato plant) in Shambat, Khartoum North, Sudan, and screened using morphological tests, biochemical and molecular characterization using 16s rDNA analysis., Screening of cellulase producing isolates was done using carboxyl methyl cellulose (CMC) as a substrate at 25°C. Twenty six isolates were found to be cellulase producers. Among the isolates, four isolates, 9+, 23, 20 and 13 showed high potential in producing extracellular cellulase and had an average cellulase activity of 2.89, 3.12, 3.48 and 3.53 Unit/ml, respectively. Genetic distance between the four isolates with high cellulase activity was determined with RAPD analysis based on OPC-3 primer.

Keywords:
bacillus cellulase biochemical identification 16s rDNA

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