Antagonistic Activity of Pseudomonas fluorescens CI on Fungal Contaminants of Groundnut (Arachis hypogaea L.) Seeds During Sale in Côte d’Ivoire

Zamblé Bi Irié Abel Boli^1, , Wahauwouélé Hermann Coulibaly¹, Rokiatou Bamba¹, Koffi Maïzan Jean-Paul Bouatenin¹ and W. Aimée Mireille Alloue-Boraud¹

¹Laboratory of Biotechnology and Food Microbiology, Department of Food Science and Technology, NANGUI ABROGOUA University, 02 BP 801 Abidjan 02, Côte d’Ivoire

Cite this paper:
Zamblé Bi Irié Abel Boli, Wahauwouélé Hermann Coulibaly, Rokiatou Bamba, Koffi Maïzan Jean-Paul Bouatenin and W. Aimée Mireille Alloue-Boraud. Antagonistic Activity of Pseudomonas fluorescens CI on Fungal Contaminants of Groundnut (Arachis hypogaea L.) Seeds During Sale in Côte d’Ivoire. American Journal of Microbiological Research. 2023; 11(4):88-96. doi: 10.12691/ajmr-11-4-1

Abstract

Post-harvest groundnut commonly used for many culinary preparations are most often subject to fungal contamination during storage in shops. In the perspective of designing an integrated control strategy for fungal spoilage contaminants in groundnut seeds, the objective of this work was to identify them and to evaluate the inhibitory capacity of the bacterium Pseudomonas fluorescens CI against these fungal contaminants. Thus, 27 samples of 500 g each of groundnut seeds were collected in the markets of three communes of Abidjan, namely Abobo, Adjamé and Yopougon. Five fungal genera including Absidia (one strain), Mucor (two strains), Rhizopus (two strains), Aspergillus (five strains) and Trichophyton (three strains) were isolated from collected groundnut seeds and subsequently pathogenicity tests of isolated fungal strains were applied to healthy groundnut seeds surface disinfected with a chlorine solution (2%). Subsequently, tests of biocontrol antagonists of P. fluorescens CI were also demonstrated by direct in vitro confrontation of P. fluorescens CI and fungal strains of isolated groundnut seeds. The results indicate that all fungal strains isolated caused partial or total spoilage of groundnut seeds within two weeks. The most significant spoilage was caused by Rhizopus sp. and Absidia sp. which caused soft rot of the seeds. However, the in vitro antifungal activity of Pseudomonas fluorescens CI showed strong inhibition of the growth of all the spoilage fungal strains when Pseudomonas fluorescens CI was inoculated 72 h before the fungal strains. These inhibition rates ranged from 52.63±3.73% to 80.42±0.42%. This indicates that Pseudomonas fluorescens CI has antifungal properties that could be used during groundnut seeds storage.

Keywords:
groundnut seeds storage fungal contamination antagonistic activity Pseudomonas fluorescens CI

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