Cellulase Producing Bacteria Isolation, Screening and Media Optimization from Local Soil Sample

Md. Arju Hossain¹, Md. Akash Ahammed¹, Saiful Islam Sobuj¹, Siratul Kubra Shifat¹ and Pratul Dipta Somadder^1,

¹Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh

Cite this paper:
Md. Arju Hossain, Md. Akash Ahammed, Saiful Islam Sobuj, Siratul Kubra Shifat and Pratul Dipta Somadder. Cellulase Producing Bacteria Isolation, Screening and Media Optimization from Local Soil Sample. American Journal of Microbiological Research. 2021; 9(3):62-74. doi: 10.12691/ajmr-9-3-1

Abstract

Cellulase enzyme earns consecutively increasing demand in industries of Bangladesh. This experiment has been planned to develop the enzyme industry in our country rather than import enzymes from other countries and conclude with isolating and screening cellulose-degrading bacteria from local land samples and ensuring maximum enzyme production through media optimization. After collecting soil samples from various Tangail areas, a typical dilution of series was performed on a Carboxymethylcellulose plate (CMC) to produce cellulase-producing microorganisms. Based on the hydrolysis zone of the ratio, four isolates were chosen and labeled CBM21, CSG8, CSR35, and CHT8 for further morphological and biochemical analysis. Then subjected to cellulase production in 250 ml of Erlenmeyer flask using CMC broth for 48 hours of fermentation at 37°C at an agitation rate of 140rpm, and cellulase enzyme assay methods evaluated crude enzymes. Eventually, those confirmed isolates were optimized using different parameters by submerged fermentation process for enhanced cellulase production. Among four isolates, CSG8 and CBM21 exhibited the maximum clearance zone with the hydrolytic values 6.96 and 8.46. These isolates were identified as Pseudomonas spp. (CSG8). According to the screening process, CBM21 (0.156U/ml), CSG08 (0.106U/ml), and E. coli (negative control) have been used for optimization with their initial enzyme productivity. After optimization, an amazing enhancement was noticed for CBM21 (1.35 U/ml) and CSG8 (1.23 U/ml) compared to E.coli (0.44 U/ml). The enzyme was characterized in isolates with an effective temperature of 40 and 35°C for CBM21 and CSG8, respectively, and pH 7, glucose 5% with the incubation time 24h was optimal. Aftermath, this type of optimization can be a significant initiative in the enzyme industries of Bangladesh.

Keywords:
ellulase isolation screening carboxymethylcellulose optimization hydrolysis

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