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American Journal of Infectious Diseases and Microbiology
ISSN (Print): 2328-4056 ISSN (Online): 2328-4064 Website: https://www.sciepub.com/journal/ajidm Editor-in-chief: Maysaa El Sayed Zaki
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American Journal of Infectious Diseases and Microbiology. 2016, 4(3), 61-71
DOI: 10.12691/ajidm-4-3-3
Open AccessArticle

Immunoinformatics Predication and in silico Modeling of Epitope-Based Peptide Vaccine Against virulent Newcastle Disease Viruses

Marwan Mustafa Badawi1, , Afra AbdElhamid Fadl Alla1, Salma Sleak Alam2, Wafa Aljack Mohamed3, Duaa Adil Nasr-Eldin Osman2, Samar Ali Abd Alrazig Ali2, Entissar Mohamed Elhassan Ahmed2, Abdah AbdElmonim Adam4, Ranya Omar Abdullah5 and Mohamed Ahmed Salih1

1Department of Biotechnology, Africa city of Technology- Khartoum, Sudan

2Omdurman Islamic university- Khartoum, Sudan

3Ibn Sina University - Khartoum, Sudan

4Elrazi University - Khartoum, Sudan

5ALneealin University- Khartoum, Sudan

Pub. Date: June 20, 2016
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Cite this paper:
Marwan Mustafa Badawi, Afra AbdElhamid Fadl Alla, Salma Sleak Alam, Wafa Aljack Mohamed, Duaa Adil Nasr-Eldin Osman, Samar Ali Abd Alrazig Ali, Entissar Mohamed Elhassan Ahmed, Abdah AbdElmonim Adam, Ranya Omar Abdullah and Mohamed Ahmed Salih. Immunoinformatics Predication and in silico Modeling of Epitope-Based Peptide Vaccine Against virulent Newcastle Disease Viruses. American Journal of Infectious Diseases and Microbiology. 2016; 4(3):61-71. doi: 10.12691/ajidm-4-3-3

Abstract

ewcastle disease virus (NDV) is negative sense single stranded RNA belongs to the Avulavirus genus of the Paramyxoviridae family which can be transmitted by inhalation or ingestion. Birds infected shed these viruses in feces as well as respiratory secretions. The aim of this study is to analyze fusion (F) protein of all virulent Newcastle strains reported in NCBI database using insilico approaches to select all possible epitopes that can be used as a therapeutic peptide vaccine. A total of 56 virulent NDV fusion protein variants retrieved from NCBI database. the conserved regions were introduced into IEDB analysis resource to predict B and T cell epitopes, as well as predicting the binding affinity of the conserved epitopes with BF2 21:01, from the predominantly expressed chicken MHC I molecule. Epitopes with high scores in both B and T cell epitopes predicting tools were suggested. Peptide vaccine against virulent NDV is strongly supersedes the conventional vaccines, as it designed to cover variant virulent mutated strains, which will reduce the recurrent outbreaks and their huge accompanied economical loss to a minimum.

Keywords:
newcastle disease virus (NDV) peptide vaccine Immune Epitope Database IEDB epitopes Vaccine

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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