Marwan Mustafa Badawi^1,, Afra AbdElhamid Fadl Alla¹, Salma Sleak Alam², Wafa Aljack Mohamed³, Duaa Adil Nasr-Eldin Osman², Samar Ali Abd Alrazig Ali², Entissar Mohamed Elhassan Ahmed², Abdah AbdElmonim Adam⁴, Ranya Omar Abdullah⁵, Mohamed Ahmed Salih¹

ewcastle disease virus (NDV) is negative sense single stranded RNA belongs to the Avulavirus genus of the Paramyxoviridae family which can be transmitted by inhalation or ingestion. Birds infected shed these viruses in feces as well as respiratory secretions. The aim of this study is to analyze fusion (F) protein of all virulent Newcastle strains reported in NCBI database using insilico approaches to select all possible epitopes that can be used as a therapeutic peptide vaccine. A total of 56 virulent NDV fusion protein variants retrieved from NCBI database. the conserved regions were introduced into IEDB analysis resource to predict B and T cell epitopes, as well as predicting the binding affinity of the conserved epitopes with BF2 21:01, from the predominantly expressed chicken MHC I molecule. Epitopes with high scores in both B and T cell epitopes predicting tools were suggested. Peptide vaccine against virulent NDV is strongly supersedes the conventional vaccines, as it designed to cover variant virulent mutated strains, which will reduce the recurrent outbreaks and their huge accompanied economical loss to a minimum.

newcastle disease virus (NDV), peptide vaccine, Immune Epitope Database IEDB, epitopes, Vaccine