First Detection of Fluoroquinolones and Aminoglycosides Resistance Genes in Multiresistant Enterobacteriaceae from Urinary Tract Infections

Nicole Prisca Makaya Dangui Nieko^{1, 2,} , Cyr Jonas Morabandza^{1, 3}, Moїse Doria Kaya-Ongoto^{1, 4}, Duchel Jeanedvi Kinouani Kinavouidi^{1, 4} and Prosvi-Esrom Bikouma Mbangou²

¹Service de Bactériologie, Laboratoire National de Santé Publique, BP 120, Brazzaville, Congo

²Ecole Normale Supérieure, Université Marien Ngouabi, BP 69, Brazzaville, Congo

³Ecole Normale Supérieure, Université Marien Ngouabi, BP 69, Brazzaville, Congo;Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo

⁴Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo

Cite this paper:
Nicole Prisca Makaya Dangui Nieko, Cyr Jonas Morabandza, Moїse Doria Kaya-Ongoto, Duchel Jeanedvi Kinouani Kinavouidi and Prosvi-Esrom Bikouma Mbangou. First Detection of Fluoroquinolones and Aminoglycosides Resistance Genes in Multiresistant Enterobacteriaceae from Urinary Tract Infections. American Journal of Infectious Diseases and Microbiology. 2022; 10(4):131-138. doi: 10.12691/ajidm-10-4-3

Abstract

Aminoglycosides and fluoroquinolones are among the most used antibiotics in synergy with beta-lactams. The objective of this work was to detect genes associated with resistance to fluoroquinolones and aminoglycosides in multiresistant strains of enterobacteria responsible for urinary tract infections. The diffusion method was used for antimicrobial sensitivity test of strains. qnr and aac genes were detected by Polymerase Chain Reaction. A total of 128 enterobacterial strains were studied. Resistance to the antibiotic families tested was observed with a frequency greater than 50%. Aminoglycosides were the least active family among the antibiotics used, with a resistance rate of 69.53%. aac(6’)-Ib-cr and qnrS genes were detected at rates of 34.09% and 22.72%, respectively. 13.63% of the strains showed coexpression of the qnr and aac genes. The detection of resistance genes to fluoroquinolones and aminoglycosides in enterobacterial strains isolated at the National Public Health Laboratory in Brazzaville could constitute a major concern in the therapeutic management of patients because it could lead to high costs for prolonged hospitalization.

Keywords:
Enterobacteriaceae multidrug resistance fluoroquinolones and aminoglycosides resistance genes urinary tract infections

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References:

[1]	Livermore D.M., 1995. Betalactamases in laboratory and clinical resistance. Clinical. Microbiology Review, 8: 557-584.
[2]	WHO, 2014. Antimicrobial Resistance: Global report on surveillance 2014. France: World Health Organization.
[3]	Wachino J. & Arakawa Y., 2012. Exogenously acquired 16S rRNA methyltransferases found in aminoglycoside-resistant pathogenic Gram-negative bacteria: an update, Drug Resistance Updates, 15: 133-148.
[4]	David C. Hooper and George A. Jacoby. Topoisomerase Inhibitors: Fluoroquinolone Mechanisms of Action and Resistance. Cold Spring Harb Perspect Med. 2016 Sep; 6(9): a025320.
[5]	Slama T. G., 2008 - Gram-negative antibiotic resistance: there is a price to pay. Crit Care, 12: S4.
[6]	OMS, 2015. Plan d’action mondial pour combattre la résistance aux antimicrobiens.
[7]	Davies Julian and Davies Dorothy. Origins and Evolution of Antibiotic Resistance. Microbiol Mol Biol Rev. 2010 Sep; 74(3): 417-433.
[8]	Baquero F, Martínez JL, Cantón R. Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol. 2008 Jun; 19(3): 260-5.
[9]	Leverstein-Van H.M.A., Dierikx C.M., Cohen S.J., Voets G.M., Van Den M.M.P., Van Essen-Zandbergen A., Platteel T., Fluit A.C., Van De S.B.N., Scharinga J., Bonten M.J.M. & Mevius D.J., 2011 - Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains. Clinical Microbiology Infection, 17: 873-880.
[10]	Bartley, P.S., Domitrovic, T.N., Moretto, V.T., Santos, C.S., Ponce-Terashima, R., Reis, M.G., Barbosa, L.M., Blanton, R.E., Bonomo, R.A., Perez, F., 2019. Antibiotic resistance in Enterobacteriaceae from surface waters in urban Brazil highlights risks of poor sanitation. Am. J. Trop. Med. Hyg. 100, 1369-1377.
[11]	Salah et al., (2019) Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo. Antimicrobial Resistance and Infection Control 8:104.
[12]	Gad G. F., Mohamed, H. A. and Ashour H.M, 2011. “Aminoglycoside resistance rates, phenotypes, and mechanisms of gram-negative bacteria frominfected patients in upper Egypt. Plos One. 6(2): e17224.
[13]	CLSI, Clinical and Laboratory Standards Institute (2021) Performance standards for antimicrobial susceptibility testing. 25th informational supplement, Clinical and Laboratory Standards Institute, Wayne, M100-S25.
[14]	Seyed M.S & Fereshteh E. 2014 - Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumonia. Jundishapur Journal of Microbiology, 7: e11136.
[15]	Robicsek, A., Strahilevitz, J., Sahm, D.F., Jacoby, G.A., Hooper, D.C., 2006c. Qnr prevalence in ceftazidime-resistant Enterobacteriacae isolates from the United States. Antimicrob. Agents Chemother. 50, 2872–2874.
[16]	Noppe-Leclercq I., Wallet F., Haentjens S., Courcol R & Simonet M. 1999 - PCR detection of aminoglycoside resistance genes: a rapid molecular typing method for Acinetobacter baumannii. Research Microbiology, 150: 317-322.
[17]	Bakour S., Kempf M., Touati A., Ait A.A., Haouchine D., Sahli F& Rolain J.M. 2012. Carbapenemase-producing Acinetobacter baumannii in two university hospitals in Algeria. Journal of Medical Microbiology, 61: 1341-1343.
[18]	Anago E, Ayi-Fanou L, Akpovi CD, Hounkpe WB, Agassounon-Djikpo Tchibozo M, Bankole HS, Sanni A. Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin. Ann Clin Microbiol Antimicrob. 2015 Jan 17; 14: 5.
[19]	Bahmani N., 2019. “Detection of VIM-1, VIM-2 and IMP-1 metallo-β-lactamase genes in Klebsiella pneumoniae isolated from clinical samples in Sanandaj, Kurdistan, west of Iran”. Iranian journal of microbiology. 11 (3): 225-231.
[20]	Abo-State, M. A. M. Salehb Y. E., Ghareebc H. M.. 2018. Prevalence and sequence of aminoglycosides modifying enzymes genes among E.coli and Klebsiella species isolated from Egyptian hospitals. Journal of Radiation Research and Applied Sciences.
[21]	Jamali L., Haouzane F., Bouchakour M., Oufrid S., Ghazlane Z., El Mdaghri N., Nadifi S., et Timinouni M., 2014. “Prevalence of plasmid mediated quinolone resistance genes among enterobacteria isolates in Moroccan community”. International Journal of Innovation and Scientific Research. ISSN 2351-8014; 11 (2): 387-399.
[22]	Makaya Dangui Nieko N. P., Morabandza, C.J., Kaya-Ongoto, M.D., Kinavouidi, D.J.K., Mikia, H.J., Kangoula-Dia-Kikouidi-Kia-Louzala, F. and Niama, F.R. (2022) Phenotypic and Genotypic Characterization of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase among Enterobacteria Isolated at National Public Health Laboratory of Brazzaville. Advances in Microbiology, 12, 363-377.
[23]	Bakiri. N., Amamra.I. 2009. « Etude de l’antibiorésistance de souche d’entérobactéries isolées des eaux polluées et en milieu hospitalier, Microbiologie général et biologie moléculaire des micro-organismes » Université des frères Mentouri Constantine. 1p.
[24]	Gatya Al-Mayahie SM, Al-Guranie DRT, Hussein AA, Bachai ZA (2022) Prevalence of common carbapenemase genes and multidrug resistance among uropathogenic Escherichia coli phylogroup B2 isolates from outpatients in Wasit Province/ Iraq. PLoS ONE 17(1): e0262984.
[25]	Romeli A., Derfoufi O., Omar C., Hajjam Z. et Zouhdi M., 2011-Enterobacteria ESBL urinary infections: epidelomiology and resistance. Maroc Medical. 33 (1): 12-16.
[26]	Okalla Ebongue C., Donggmo Tsiazok M., Nda Mefo’o J P Ngaba G P Beyiha Get Adiogo D 2015- Evolution de la résistance aux antibiotiques des entérobactéries isolées à l’hopital gén éral de Douala de 2005 à 2012. The pan African Medical Journal. 25(227): 1-11.
[27]	Decré D., 2012. “Acinetobacter baumannii et résistance aux antibiotiques: un modèle d’adaptation”. Revue francophone des laboratoires. 441: 43-52.
[28]	Bradford P. A. 2001. “Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat”. Clinical Microbiology Reviews. 14: 933-951.
[29]	Amirkamali S., Naserpour-Farivar T., Azarhoosh K&Peymani A. 2017. Distribution of the blaOXA, blaVEB-1, and blaGES-1 genes and resistance patterns of ESBL producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, ran. Revista Da Socieda de Brasiliera De Medicina Tropical, 50: 315-320.
[30]	Barbier F & Wolff M. 2010 - Multirésistance chez Pseudomonas aeruginosa: Vers l’impasse thérapeutique? Médecine-Sciences, 26: 960-968.
[31]	Ferjani A, Mkaddemi H, Tilouche S, Marzouk M, Hannechi N, Boughammoura L, Boukadida J. “Caractéristiques épidémiologiques et bactériologiques des bactéries uropathogènes isolées dans un milieu pédiatrique”. Archives de Pédiatrie. 2011; 18: 230-4.
[32]	Zaunbrecher MA, Sikes RD Jr, Metchock B, Shinnick TM, Posey JE (2009) Overexpression of the chromosomally encoded aminoglycoside acetyl-transferase eis confers kanamycin resistance in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 106(47): 20004-20009.
[33]	Teixeira B, Rodulfo H, Carreno N, Guzman M, Salazar E, Donato MD (2016) Aminoglycoside resistance genes in Pseudomonas aeruginosa isolates from Cumana, Venezuela. Rev Inst Med Trop Sao Paulo 58.
[34]	Mendes RE, Castanheira M, Toleman MA, Sader HS, Jones RN, Walsh TR et al (2007) Characterization of an integron carrying blaIMP-1 and a new aminoglycoside resistance gene, aac (6′)-31, and its dissemination among genetically unrelated clinical isolates in a Brazilian hospital. Antimicrob Agents Chemother 51(7): 2611-2614.
[35]	Lindemann PC, Risberg K, Wiker HG, Mylvaganam HJA (2012) Aminoglycoside resistance in clinical Escherichia coli and Klebsiella pneumoniae isolates from Western Norway. Apmis 120(6): 495-502.
[36]	Martinez-Martinez L., Pascual A. & Jacoby G.A., 1998 - Quinolone resistance from a transferable plasmid, Lancet; 351: 797-9.
[37]	Strahilevitz J, Jacoby GA, Hooper DC, Robicsek A. Plasmid-mediated quinolone resistance: a multifaceted threat. Clin Microbiol Rev. (2009) 22: 664-89.
[38]	Mahmud ZH, Kabir MH, Ali S, Moniruzzaman M, Imran KM, Nafiz TN, Islam MS, Hussain A, Hakim SAI, Worth M, Ahmed D, Johnston D and Ahmed N (2020) Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Drinking Water Samples From a Forcibly Displaced, Densely Populated Community Setting in Bangladesh. Front. Public Health 8: 228.
[39]	Guessennd N., Bremont S., Gbonon V., Kacou-Ndouba A., Ekaza E., Lambert T., Dosso M. et Courvalin P., 2008-Qnr-type quinolone resistance in extended-spectrum bêta-lactamases producing Enterobacteria in Abidjan Ivory Coast. Pathologie Biologie. 56: 439-446.
[40]	Makaya Dangui Nieko P.N., et al., 2017. Phenotypical characterization of Pseudomonas aeruginosa clinical strains isolated in Abidjan (Côte d’Ivoire). Microbiology Research Journal International. 22(2): 1-10, 2017.
[41]	Tahou E. J., Guissennd K. N., GboNon V., Gha K. M. K., Koman F., Tiekoura K. B., Ouattara M. B. Toty A. et Dosso M., 2017. Caractérisation moléculaire des intégrons des classe 1, 2 et 3 et transfert horizontal de la résistance aux antibiotiques chez Klebsiella pneumoniae à Abidjan Côte d’Ivoire. Institut Pasteur de Côte d’Ivoire. Abidjan. 172p.
[42]	Levy SB & Marshall B. 2004. Antibacterial resistance worldwide: causes, challenges and responses. Nat Med.; 10: S122-129.