Evaluation of the Toxicity of Hemizygia bracteosa (Benth) Plant Used in Traditional Medicine for the Treatment of Diabetes Mellitus in Benin

Nicodème W CHABI¹, Christian T. R. KONFO^2, , Marius ADJAGBA³, Loukman MOUSSEDIKOU², Edwige AHOUSSI-DAHOUENON², Anatole LALEYE³, Christophe GBAGUIDI⁴ and Mohamed Mansourou SOUMANOU²

¹Laboratory of Teaching and Research in Food Microbiology, DSepartment of Food Technology Engineering, Polytechnic School of Abomey, University of Abomey, 01P.BP 2009 Cotonou, Benin

²Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey, University of Abomey-calavi, 01 BP 2009 Cotonou, Benin.

³Laboratory of Cytogenetic, Human Biology Unit, Faculty of Health Sciences / University of Abomey-calavi, Benin

⁴Association for the Promotion of Tropical Medicinal Plants (APTMP), 03 BP 246 Cotonou, Benin

Cite this paper:
Nicodème W CHABI, Christian T. R. KONFO, Marius ADJAGBA, Loukman MOUSSEDIKOU, Edwige AHOUSSI-DAHOUENON, Anatole LALEYE, Christophe GBAGUIDI and Mohamed Mansourou SOUMANOU. Evaluation of the Toxicity of Hemizygia bracteosa (Benth) Plant Used in Traditional Medicine for the Treatment of Diabetes Mellitus in Benin. American Journal of Biomedical Research. 2015; 3(3):40-44. doi: 10.12691/ajbr-3-3-2

Abstract

This study aims to assess the toxicity of Hemizygia bracteosa (Benth), a medicinal plant used for the treatment of diabetes mellitus in Benin. Wistar rats were force-feeded by using solutions obtained by dissolving the powder of this plant in distilled water. The assessment of biochemical parameters (blood glucose, triglycerides, cholesterol, HDL cholesterol, proteins) of Wistar rat serum was used to highlight the hypoglycaemic properties of this plant and its effects on cardiovascular risk factors. The results showed an increase in HDL-cholesterol levels (0.13 ± 0.004 vs 0.48 ± 0.15) and a decrease in total cholesterol levels (1.06 ± 0.40 vs 0.81 ± 0.12), triglycerides (1.2 ± 0.43 vs 1.18 ± 0.38), glucose (1.60 ± 0.25 vs 0.98 ± 0.24) and proteins (95. 33 ± 20.59 vs 58.30 ± 6.26) in rats serum after one month treatment. No significant differences were observed in control rats for all parameters. our data also shows that at concentrations from 2 g/kg this plant appears topresent significant toxicity on kidneys and liver. The use of this plant for diabetes treatment must be done with caution and doses must be controlled.

Keywords:
diabetes mellitus traditional medicine toxicity Hemizygia bracteosa (Benth) Benin

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References:

[1]	Holm, G., V. Herbst, and B. Teil Brogenkundé, In: Planta Medica 67:263-269. 1998.
[2]	Kokwaro, J.O, Medicinal Plants of East Africa, Second Edition, Kenya Literature Bureau, Nairobi. 1993.
[3]	Elqaj, M., Ahami, A., & Belghyti, D, La phytothérapie comme alternative à la résistance des parasites intestinaux aux antiparasitaires. Journée scientifique" ressources naturelles et antibiotiques". Maroc. Juin. 2007.
[4]	OMS, (2002), la phytothérapie comme alternative à la résistance des parasites intestinaux aux antiparasitaires. Journée Scientifique « Ressources Naturelles et Antibiothérapie », Faculté des Sciences-Kenitra. 22 Juin 2007
[5]	India Council Of Medical Research, Quality Standards of India Medical plants, Volume 1. Ansari Nagar New Delhi-110029, India. 2003.
[6]	Wild, S., G. Roglic., A. Green., R. Sicree and King, H, Global prevalence of diabetes estimates for the year 2000 and projections for 2030. Diabetes care, 27(5), 1047-1053. 2004.
[7]	Maiti, R., D.Jana., U. K. Das and D. Ghosh, Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 92(1), 85-91. 2004.
[8]	Iwu, M.M., A.R. Duncan., and C.O. Okunji, New Antimicrobials of plant.Origin.J.Janick(ed), ASHS Press, Alexandria, VA.Egypt. 1999.
[9]	Konfo, T.R.C., N.W. Chabi, J. Agbadjizo, E. Dahouenon-Ahoussi, M.M. Soumanou, and D.C.K. Sohounhloue, Influence de la feuille de Hemizygia bracteosa (Benth) sur la qualité de la bière du sorgho « tchakpalo » produite au Bénin. International Journal of Innovation and Applied Studies. 7 (2), 453-463. Aug.2014.
[10]	Cantino P.D., R.M. Harley., and S.J. Wagstaff, Genera of Labiatae: Status and classification. In: Harley R., M., Reynolds T. (eds) Advances in Labiatae science. Royal Botanic Gardens, Kew. 511-522. 1992.
[11]	Paton A. and K. Balkwill, Hemizygiastalmansii (Labiatea), a new species from Mpumalanga, South Africa and Swaziland Kew-Bull. 56: 491-496. 2001.
[12]	Hutchinson, J., and J. M Dalziel, Flora of Tropical West AfricaCrown agents for the ColoniesLondon. 1931.
[13]	Fossati, P. and L. Prencipe, Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry, 28(10): 2077-2080. 1982.
[14]	Doumas, B.T., D.D. Bayse., R.J. Carter., T. Peters. and R. Schaffer, A candidate reference method for determination of total protein in serum. I. Development and validation. Clinical Chemistry; 27(10): 1642-1650. 1981.
[15]	Oecd. (1994). OECD Guidelines for the Testing of Chemicals. Organization for Economic. (Section 4: Health Effects; Test No. 423: Acute Oral Toxicity-class method adopted on 17 December 2001.
[16]	Otieno, D. F., K. Balkwill. And A. J. Paton, A Multivariate analysis of the Hemizygia bracteosa complex. Plant Systematics and Evolution. 261: 19 – 38. 2006.
[17]	Chabane, D., F. Saidi., A. Rouibi. et K. Azine, Activité hypoglycémique de l’extrait aqueux d’Ajugaiva L. schreber chez les rats diabétiques induite par l’alloxane. Afrique Science 09 (1) 120-127. 2013.
[18]	Eddouks, M., A. Lemhadri., N.A. Zegghwagh. and J.B. Michel, Potent hypoglycemic activity of the aqueous extract of Chamaemelumnobile in normal in normal and streptozotocin-induced diabetic rats, Diabetes Res. Clin. Pract, 67.189-195. 2005.
[19]	Alaoui, J.F., Y. Lagorce., M. Cherrah., H. Amarouch. and M. Roquehert, Activité analgésique et anti-inflammatoire des saponines d’arganiaspinosa, in : Annales pharmaceutiques Françaises, 220-228. 1998.
[20]	Deraedt, R., S. Journey, J. Benzouni. and M. Peterfalvi, Release of prostaglandins E and F in algogenic reaction and inhibition, Eur. J. 1980; 61: 17-24. Fitoterapia , 80 475-477. 2009.
[21]	Areej, M., S.P. Al-Taweel., A.F. Ghada., A. Saleh Ibrahim. and E.H. Kamal El Tahir, New flavane gallates isolated from the leaves of Plicosepaluscurviflorus and their hypoglycemic activity, Fitoterapia, 83, 1610-1615. 2012.
[22]	Deutschländer, M.S., N. Lall., M. Van de Venter. and S. Dewanjee, The hypoglycemic activity of EucleaundulataThunb. var. myrtina (Ebenaceae) root bark evaluated in a streptozotocin–nicotinamide induced type 2 diabetes rat model. South African Journal of Botany, 80, 9-12. 2012.
[23]	Antenne Médicale de Prévention du Dopage (AMPD), Les risques des plantes médicinales, Languedoc Roussillon, Lettre d'Actualités de septembre 2007.