Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia

Cut Muthiadin¹, Rosdiana Natsir², Rosana Agus³, Muhammad Nasrum⁴, Ressy Dwiyanti⁵, Muhammad Sabir⁵, Yadi Yasir⁶ and Mochammad Hatta^4,

¹Department of Biology, Islam Government University, Makassar, Indonesia

²Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

³Department of Microbiology, Faculty of Life Sciences, Hasanuddin University, Makassar, Indonesia

⁴Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

⁵Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

⁶Department of Microbiology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia

Cite this paper:
Cut Muthiadin, Rosdiana Natsir, Rosana Agus, Muhammad Nasrum, Ressy Dwiyanti, Muhammad Sabir, Yadi Yasir and Mochammad Hatta. Identification and Characterization of Antigenic 36 Kda Outer Membrane Protein (OMP) of Salmonella entericaserovar Typhi (S.typhi) from Makassar, South Sulawesi, Indonesia. American Journal of Biomedical Research. 2015; 3(1):9-12. doi: 10.12691/ajbr-3-1-3

Abstract

Typhoid fever caused by Salmonella entericaserovar Typhi (S. typhi), still remains a public health concern. A simple, rapid and early diagnostic test has been a long felt need for clinicians. Adh036 protein, discovered from Malang, Indonesia, has a candidate vaccine potential for Typhoid Fever. This study aims towards identification and characterization of 36 kda Outer Membrane Protein (OMP) from Makassar, South Sulawesi, Indonesia as a generalization protein test. Isolated bacteria were cultured in MacConkey medium and Brain Heart Infusion Broth (BHIB), and then harvested by using sarcosyl and sonication methods. Then, crude protein was purified by dialysis method using fractional ammonium sulfate precipitation and the protein contents were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophores (SDS-PAGE) for identification it’s molecular weight. The results, from five purified proteins, only three fractions show band at 36 kda, were 0-20%, 20-40%, and 40-60%.

Keywords:
Outer Membrane Protein (OMP) Salmonella entericaserovar Typhi (S. typhi) Purification SDS-PAGE

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