Study of Soil Actynomycetes and Their Antagonastic Activity against Entric Fever Pathogens

Oliver Madhale^1, , Vinay Chougule² and Ujwala Mane³

¹Department of Microbiology, Rayat Institute of Research and Development, Satara, & Shivaji University, Kolhapur, Maharashtra, India

²Department of Microbiology, MirajMahavidyalaya, Miraj, Maharashtra, India

³PG Department of Microbiology, Yashwantrao Chavan Institute of Science, and Rayat Institute of Research and Development, Satara, Maharashtra, India

Cite this paper:
Oliver Madhale, Vinay Chougule and Ujwala Mane. Study of Soil Actynomycetes and Their Antagonastic Activity against Entric Fever Pathogens. Applied Ecology and Environmental Sciences. 2022; 10(2):60-64. doi: 10.12691/aees-10-2-5

Abstract

Actinomycetes were isolated from agricultural and non-agricultural farm soil samples in the Sangli district of Maharashtra. 60 actinomycetes were isolated from 30 soil samples, with only three isolates from agricultural soil and three from non-agricultural soil displaying inhibitory activity. Soil samples from non-agriculture farms yielded a higher percentage of antagonistic actinomycetes (10%) than those from agriculture farms (2 %). Three non-agricultural isolates with high inhibitory activity were tested for their inhibitory profile against three human test pathogens, Salmonella typhi, S. paratyphi A, and paratyphi B. The actinomycete isolate INA - 53 exhibited a high degree of inhibitory activity against all the test pathogens both in terms of the number of test pathogens inhibited and the zone of inhibition as compared to the standard antibiotic used that is streptomycin. INA - 53 was identified to be belonging to the genus Streptomyces spp. The results suggest that non agriculture soil samples are rich in organic matter with a high C:N ratio are the potential source of antagonistic actinomycetes and a large number of antagonistic actinomycetes producing anti-microbial compounds can be isolated from non agriculture soil.

Keywords:
Actinomycetes antibacterial activity antibiotics human pathogens

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References:

[1]	R. N. Wadetwar and A. T. Patil, “Isolation and characterization of bioactive actinomycetes from soil in and around Nagpur,” International Journal of Pharmaceutical Sciences and Research, 2013; vol. 4, no. 4, p. 1428.
[2]	Bérdy J. Bioactive microbial metabolites: A personal view. J Antibiot (Tokyo) 2005; 58: 1-26.
[3]	Okami, Y. & Hotta, K. Search and discovery of new antibiotics, In: Goodfellow, M., Williams S.T. and Mordarski, M. (Eds.), Actinomycetes in biotechnology. Academic Press, Inc., New York, 1988; pp.33-67.
[4]	Luzhetskyy, A., Pelzer, S., Bechthold, A. The future of natural products as a source of new antibiotics. Curr. Opin. Invest. Drugs, 2007; 8: 608-613.
[5]	Kumar N, Singh R.K. and Mishra S.K. et al. Isolation and screening of soil actinomycetes as sources of antibiotics active against bacteria. International Journal of Microbiology Research, 2010, 2: 12-16.
[6]	Lange L, Breinholt J, Rasmussen FW, Nielsen RI.Microbial fungicides.the natural choice. Pestic Sci. 1993; 39:155-160.
[7]	Pandey, B., Ghimire, P. and Agrawal, V.P. International Conference on the Great Himalayas: Climate, Health, Ecology, Management and Conservation, Kathmandu, Organized by Kathmandu University and the Aquatic Ecosystem Health and Management Society, Canada 2004 .
[8]	Krishnakumari K, Ponmurugan P, Kannan N Isolation and characterization of Streptomyces sp. for secondary metabolite production. Biotechnology 2006; 5:478-480.
[9]	Ponmurugan P, Nithya B. Plasmid DNA of antibiotic producing strains of Streptomyces sannanensis. Biotechnology 2008; 7: 487-492.
[10]	Xu LH, Jiang Y, Li WJ, Wen ML, Li MG, Jiang CL. Streptomyces roseoalbus sp. nov., an actinomycetes isolated from soil in Yunnan, China. Antonie Van Leeuwenhoek 2005; 87: 189-194.