1Department of Microbiology, Rayat Institute of Research and Development, Satara, & Shivaji University, Kolhapur, Maharashtra, India
2Department of Microbiology, MirajMahavidyalaya, Miraj, Maharashtra, India
3PG Department of Microbiology, Yashwantrao Chavan Institute of Science, and Rayat Institute of Research and Development, Satara, Maharashtra, India
Applied Ecology and Environmental Sciences.
2022,
Vol. 10 No. 2, 60-64
DOI: 10.12691/aees-10-2-5
Copyright © 2022 Science and Education PublishingCite this paper: Oliver Madhale, Vinay Chougule, Ujwala Mane. Study of Soil Actynomycetes and Their Antagonastic Activity against Entric Fever Pathogens.
Applied Ecology and Environmental Sciences. 2022; 10(2):60-64. doi: 10.12691/aees-10-2-5.
Correspondence to: Oliver Madhale, Department of Microbiology, Rayat Institute of Research and Development, Satara, & Shivaji University, Kolhapur, Maharashtra, India. Email:
oliver.paul.madhale@gmail.comAbstract
Actinomycetes were isolated from agricultural and non-agricultural farm soil samples in the Sangli district of Maharashtra. 60 actinomycetes were isolated from 30 soil samples, with only three isolates from agricultural soil and three from non-agricultural soil displaying inhibitory activity. Soil samples from non-agriculture farms yielded a higher percentage of antagonistic actinomycetes (10%) than those from agriculture farms (2 %). Three non-agricultural isolates with high inhibitory activity were tested for their inhibitory profile against three human test pathogens, Salmonella typhi, S. paratyphi A, and paratyphi B. The actinomycete isolate INA - 53 exhibited a high degree of inhibitory activity against all the test pathogens both in terms of the number of test pathogens inhibited and the zone of inhibition as compared to the standard antibiotic used that is streptomycin. INA - 53 was identified to be belonging to the genus Streptomyces spp. The results suggest that non agriculture soil samples are rich in organic matter with a high C:N ratio are the potential source of antagonistic actinomycetes and a large number of antagonistic actinomycetes producing anti-microbial compounds can be isolated from non agriculture soil.
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