Characterization and Antimicrobial Susceptibility Patterns of Vibrio cholerae and Vibrio cholerae O₁ Isolated in Tropical Waters: A Contribution to Cholera Disease Prevention in Equatorial Region

Blandine Pulchérie Tamatcho Kweyang^{1, 2,} , Antoine Tamsa Arfao², Pierre René Fotsing Kwetche³, Luciane Marlyse Moungang², Mamert Fils Onana⁴, Siméon Tchakonté², Mireille Ebiane Nougang⁵, Olive Vivien Noah Ewoti² and Ajeagah Gideon Aghaindum²

¹Department of Microbiology, University of Yaounde 1, Faculty of Sciences, P.O. Box 812 Yaounde, Cameroon

²Hydrobiology and Environment Laboratory, University of Yaounde 1, Faculty of Sciences, P.O. Box 812 Yaounde, Cameroon

³Higher Institute of Health Science, Université des Montagnes; Bangangté, Cameroon

⁴Department of Aquatic Ecosystems Management, Institute of Fisheries and Aquatic Sciences, University of Douala, Douala, Cameroon

⁵Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O Box. 814 Maroua, Cameroon

Cite this paper:
Blandine Pulchérie Tamatcho Kweyang, Antoine Tamsa Arfao, Pierre René Fotsing Kwetche, Luciane Marlyse Moungang, Mamert Fils Onana, Siméon Tchakonté, Mireille Ebiane Nougang, Olive Vivien Noah Ewoti and Ajeagah Gideon Aghaindum. Characterization and Antimicrobial Susceptibility Patterns of Vibrio cholerae and Vibrio cholerae O₁ Isolated in Tropical Waters: A Contribution to Cholera Disease Prevention in Equatorial Region. Applied Ecology and Environmental Sciences. 2021; 9(6):599-606. doi: 10.12691/aees-9-6-4

Abstract

The isolation and characterization of Vibrio strains were carried out in Yaounde (equatorial area). Well water characteristic focused on water temperature, pH, dissolved oxygen, dissolved CO₂, electrical conductivity, suspended solids. Vibrio cholerae, Vibrio fluvialis, Vibrio mimicus, Vibrio parahaemolyticus and Vibrio vulnificus were identified in all four wells investigated. On 50 strains of Vibrio cholerae tested, 8 agglutinated anti O₁ serum; 16% therefore belong to serogroup O₁. Antimicrobial susceptibility tests showed that most of the environmental strains of Vibrio cholerae studied are sensitive to the majority of the antibiotics tested. The percentage of sensitivity for most of the antibiotics tested is above 50%. CPR (98%); CTX (94%); GM (84%); AMC (82%); DOX (72%); AMX (70%); NAL (52%). There were 34% cases of resistant to nalidixic acid, 26% to amoxicillin, 24% to chloramphenicol, and 18% to doxycycline. The percentage of sensitivity of Vibrio cholerae O₁ is always greater than 50% with GM (100%); AMX and DOX (87.5%); CTX and CPR (75%); AMC and NAL (62.5%); CHL (50%). These results contribute to prevention and control of cholera in equatorial region.

Keywords:
isolation characterization Vibrio strains antimicrobial susceptibility physico-chemical parameters well

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