Ping Yu¹, Xiangbo Min¹, Lina Li¹, Yu Wang², Di Zhao^1,

This study aimed to evaluate the therapeutic effect of black wolfberry (Lycium ruthenicum Murr.) ferment on mice with androgenetic alopecia (AGA). Mice were arbitrarily clustered into four groups: control (C), model (M), low-dosage ferment (LF), and high-dosage ferment (HF) groups. Mice in the M, LF, and HF groups had AGA induced for 16 d. Then, the C group was treated with normal saline for 16 d; and LF and HF mice received daily doses of 2.5 and 25 mL/kg body weight of black wolfberry ferment, respectively. The results indicate that black wolfberry ferment increased the weight of newborn hair on mice with AGA. It also enhanced the number of hair follicles, terminal hairs, and vellus hairs, and increased the dermal and hypodermal thickness, as verified by the proliferation of hair follicles expressing Ki-67 and hair follicle stem cell marker Sox9. A total of 1,636 genes were selected as necessary genes using differential gene expression and weighted gene co-expression network analysis. The KEGG analysis showed that the promotion of hair regrowth was most likely associated with the PI3K/AKT, NF-kappa B, and MAPK signaling pathways. Black wolfberry ferment can be used as a therapeutic agent for AGA, and the potential genes and pathways involved in its action were identified using transcriptomics.

androgenic alopecia, Lycium ruthenicum Murr., ferment, transcriptomics, enrichment analysis