Cyclooxygenase Expression in Canines Following Peripheral Nerve Injury

Shigeru Kobayashi^{1, 2,} , Yoshihiko Suzuki³, Syuichi Yamada⁴, Naji Al-Khudairi⁵ and Adam Meir⁵

¹Department of Orthopedics and Rehabilitation Medicine, Faculty of Medical Sciences, The University of Fukui, Fukui, Japan

²Research and Education Program for Life Science, The University of Fukui, Fukui, Japan

³Department of Orthopedic Surgery, Suzuki Orthopaedic Clinics, Toki, Gifu, Japan

⁴Department of Orthopedic Surgery, Yamada Orthopaedic Clinics, Hamamatsu, Shizuoka, Japan

⁵Victor Horsley Department of Neurosurgery, The National Hospital for Neurology and Neurosurgery, Queen Square, London, UK

Cite this paper:
Shigeru Kobayashi, Yoshihiko Suzuki, Syuichi Yamada, Naji Al-Khudairi and Adam Meir. Cyclooxygenase Expression in Canines Following Peripheral Nerve Injury. International Journal of Clinical and Experimental Neurology. 2015; 3(2):26-31. doi: 10.12691/ijcen-3-2-1

Abstract

In order to investigate the mechanism of neurogenic pain, this study used a median nerve compression model in dogs. The nerve was compressed with a clip for three weeks. Immunohistochemistry was done by the avidin-biotin-peroxidase complex method to observe the changes of T cells (CD45) and macrophages (Mac-1) after compression. Antibodies against cyclooxygenase (COX)-1 and 2 were used to examine the localization and changes of these mediators caused by nerve compression. In control animals, resident T cells were detected, but there were no macrophages. COX-2 was positive in the Schwann cells and vascular endothelial cells, while COX-1 was detected in the vascular endothelial cells. After nerve compression, numerous T cells and macrophages appeared among the demyelinized nerve fibers. The macrophages were positive for COX-2. COX-2 may be deeply involved in neuritis arising from mechanical compression, and this mediator seems to be important in the manifestation of neurogenic pain.

Keywords:
peripheral nerve wallerian degeneration macrophage cyclooxigenase entrapment neuropathy

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