The Effect of Hypermagnesemia on Platelet Aggregation in Peritoneal Dialysis Patients: An in Vitro Study

Serkan Bakirdogen^1, , M.Baki Cekmen², Umit Bilgili³, Ozgur Mehtap⁴, Necmi Eren⁵, Sibel Gokcay Bek⁵, Ferruh Kemal Isman², Sara Yavuz⁶, Mehmet Tuncay⁷ and Coskun Bakar⁸

¹Department of Nephrology, Canakkale Onsekiz Mart University, The School of Medicine, Canakkale, Turkey

²Department of Medical Biochemistry, Medeniyet University, The School of Medicine, Istanbul, Turkey

³Department of Medical Biochemistry, Fatih Sultan Mehmet Educating and Research Hospital, Istanbul, Turkey

⁴Department of Hematology, Kocaeli University, The School of Medicine, Kocaeli, Turkey

⁵Department of Nephrology, Kocaeli University, The School of Medicine, Kocaeli, Turkey

⁶Division of Nephrology, Department of Internal Medicine, Yeditepe University, The School of Medicine, Istanbul, Turkey

⁷Department of Nephrology, Ersin Arslan Educating and Research Hospital, Gaziantep, Turkey

⁸Department of Public Health, Canakkale Onsekiz Mart University, The School of Medicine, Canakkale, Turkey

Cite this paper:
Serkan Bakirdogen, M.Baki Cekmen, Umit Bilgili, Ozgur Mehtap, Necmi Eren, Sibel Gokcay Bek, Ferruh Kemal Isman, Sara Yavuz, Mehmet Tuncay and Coskun Bakar. The Effect of Hypermagnesemia on Platelet Aggregation in Peritoneal Dialysis Patients: An in Vitro Study. American Journal of Medical Sciences and Medicine. 2019; 7(1):9-12. doi: 10.12691/ajmsm-7-1-3

Abstract

Magnesium has a reducing effect on platelet aggregation. Decreased glomerular filtration rate in chronic renal failure contributes to elevation of serum magnesium level. The aim of our study was to measure platelet aggregation in peritoneal dialysis (PD) patients who developed hypermagnesemia by the addition of magnesiun sulphate (MgSO₄) in vitro. Forty-three patients with PD were included in the study. Multiple electrode aggregometry method capable of measuring platelet aggregation in whole blood was used and adenosine diphosphate (ADP) was selected as the agonist. Only ADP was used in the first test cell (C1) of the device; ADP was added as a whole blood agonist, which was raised to magnesium level 3-3,5 mg/dl by the addition of in vitro MgSO₄ in the second test cell (C2). Three criteria were used to measure platelet aggregation: aggregation, area under the curve (AUC), and velocity. There was a statistically significant positive correlation between C1-C2 AUC and C1-C2 velocitiy. There was no statistically significant relationship between C1-C2 aggregation. PD patients were divided into two groups, male and female, and the results were examined. In male patient group; there was no statistically significant difference between C1-C2 AUC, C1-C2 aggregation and C1-C2 velocity. In the female patient group; there was no statistically significant difference between C1-C2 AUC. There was a statistically significant positive correlation between C1-C2 aggregation and C1-C2 velocities. Serum magnesium levels may be effective on platelet aggregation in female patients with PD but no similar effect was found in male patients.

Keywords:
hypermagnesemia platelet aggregation multiple electrode aggregometry chronic renal failure peritoneal dialysis

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