Isolation and Molecular Identification of New Emergent Candida Lusitaniae Isolated from Sudanese Immunocompromised Patients Infected with Oropharyngeal Candidiasis

Mutaz F. Saad^1, and Amr M. Albasha²

¹Department of Clinical Laboratory, College of Applied medical sciences, Al Jouf University, Sakaka, Saudi Arabia

²Department of Microbiology, College of Medical laboratory, Sudan University of science and technology, Khartoum, Sudan

Cite this paper:
Mutaz F. Saad and Amr M. Albasha. Isolation and Molecular Identification of New Emergent Candida Lusitaniae Isolated from Sudanese Immunocompromised Patients Infected with Oropharyngeal Candidiasis. American Journal of Microbiological Research. 2015; 3(2):62-64. doi: 10.12691/ajmr-3-2-4

Abstract

Seventy seven oral swab samples (n=77) were collected in period between august 2007 to may 2008 from hospitalized immunocompromised and HIV patients suspected for Oropharyngeal Candidiasis and admitted in different hospitals in Ed-wiuem state and Khartoum state, Sudan. All samples were inoculated on Sabouraud dextrose agar and identified by colonial morphology, Germ tube test and Vitek2 compact system for biochemical identification and antifungal susceptibility test. Out of 77 oral swab samples collected from immunocompromised and HIV patients, 41 (53.3%) samples showed positive growth of Candida, and 36 (46.7%) samples showed negative growth. The identification showed that out of forty one positive cultures, 32 isolates found as Candida albicans (78%), while nine samples (n=9) appeared as non-Candida albicans (22%) and found as Candida lusitaniae according to GTT and Vitek2 Compact identification. Then DNA was extracted from all non-Candida albicans isolates and DNA sequencing was carried and D1/D2 region were determined using NL1 primer. DNA based identification showed that all nine (n=9) GTT negative isolates were Candida lusitaniae (Anamorh Clavispora lusitaniae). This study documented that there are new emergent species of Candida should be considered when dealing with specimen collected from patients suspected for yeast infections. Our results provide useful information that C. lusitaniae can be isolated as well as other Candida species from immunocompromised patients in Sudan.

Keywords:
immunocompromised HIV C. lusitaniae DNA sequencing Sudan

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