Indoor Airborne Microflora in Various Section of a Tertiary Healthcare Centre in Rural Area of Ovia Northeast Edo State

Seto Saint T. ALADENIKA^1, and Mathew F. Olaniyan¹

¹Department of Medical Laboratory Science, Achievers University, Owo-Nigeria

Cite this paper:
Seto Saint T. ALADENIKA and Mathew F. Olaniyan. Indoor Airborne Microflora in Various Section of a Tertiary Healthcare Centre in Rural Area of Ovia Northeast Edo State. American Journal of Infectious Diseases and Microbiology. 2014; 2(4):86-90. doi: 10.12691/ajidm-2-4-3

Abstract

This study was carried out to determined the variation in microbiota of five section of Igbinedion University Teaching Hospital Okada. Samples were collected using the settled plate techniques for the enumeration of bacterial and fungal isolates. The air specimens were collected three times; in the morning between the hours of 8 am and 10 am, in the afternoon between 12 noon and 2 pm and in the evening between the hours of 4pm and 6pm. The total heterotrophic microbial population of the five different units (wards) studied varied from wards to wards. The highest bacterial population was recorded in the afternoon between time 12 pm and 2 pm compared to the morning and evening, ranging from 5 cfu/m3 to 17 cfu/m3, with the accident and emergency ward recording the highest bacterial counts of 17 cfu/m3. The concentration of fungal population in air of the five different wards also followed the bacteria pattern in which highest fungi population were recorderd in afternoon (16 cfu/m3). At the three different times of study, the Accident and emergency ward, male ward, female ward as well as children wards show high yield fungal isolates. Five bacteria were isolated, namely; Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes and Proteus mirabilis and identified accordingly. Aspergillus sp, Penicillum sp, and Mucor sp, were three fungi genera isolated. The degree of frequency of microbial distribution was high in the accident and emergency ward but lower in operating room (theatre). Accident and emergency ward my serve as source of infection in this hospital hence there should be an increase in level of hygiene in this unit also human movement should be limited scince they often serve as souces of pathogen.

Keywords:
indoor airborne microflora tertiary healthcare pathogen rural area

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References:

[1]	Chuaybamroong P, Choomseer P, Sribenjalux P (2008). Comparison between hospital single air unit and central air unit for ventilation performances and airborne microbes. Aerosol and Air Qual. Res, 8 (1): 28-36.
[2]	McCarthy J, Luscuere P, Streifel A, Kalliokoski P (2000). Indoor air quality in hospitals and other health care facilities. In Proceeding of Healthy Buildings 2000, Espoo, Finland.
[3]	Obbard J P, Fang L S (2003). Airborne concentrations of bacteria in a hospital environment in Singapore. Water, Air, and Soil Pollution 144: 333-341.
[4]	Atlas RM (1995). Microorganisms and Human Diseases. In Microorganisms in Our World. Mosby-Year Book, Inc. P19-22
[5]	Hambraeus A (1988). Aerobiology in the operating room– a review. J. Hosp.Infect. 11 (supp. A): 68-76.
[6]	Emmerson AM (1995). The impact of surveys on hospital infection. J. Hosp. Infect. 30: 421-440.
[7]	Gillette B (2000). Indoor air quality important. Mississippi Bus. J. 22 (47): 14-16.
[8]	Nevalainen AK, Willeke F, Liebhaber J, Pastuszka A, Burge H, Henningson E (1993). Bioaerosol sampling. In K. Willeke and P. Baron (ed.), Aerosol measurement: principles, techniques and applications. Van Nostrand Reinhold, New York. p 321-324.
[9]	Augustowska M, Dutkiewicz J (2006). Variability of airborne microflora in a hospital ward within a period of one year. Ann. Agric. Environ. Med. 13: 99-1 06.
[10]	Matar GM, Chaar MH, Araj GF, Srour Z, Jamaleddine G, Hadi U (2005). Detection of a highly prevalent and potentially virulent strain of Pseudomonas aeruginosa from nosocomial infections in a medical center. BMC Microbiol. 5: 29-36.
[11]	Montz JR, Edward W (2000). Contamination control in hospitals. Engineered Systems, 17 (6): 68-71.
[12]	Abussaud MJ (1991). Prevalence of nosocomial infections in Saudi Arabian teaching hospital. J. Hosp. Infect. 17: 235-238.
[13]	Shintani H, Taniai E, Miki A, Kurosu S, Hayashi F (2004). Comparison of the collecting efficiency of microbiological air samplers. J. Hosp. Infect. 56: 42-48.
[14]	Li CS, Hou PA (2003). Bioaerosol characteristics in hospital clean rooms. Sci. Total Environ. 305: 169-176.
[15]	Pasquarella C, Pitzurra O, Savino A (2000). The index of microbial air contamination. J. Hosp. Infect. 46: 241-256.
[16]	Dharan S, Pittet D (2002). Review: Environmental controls in operating theatres. J. Hosp. Infect. 51: 79-84.
[17]	Qudiesat, K, Abu-Elteen, K, Elkarmi A, Hamad, M, Abussaud, M.(2009) Assessment of airborne pathogens in healthcare settings African Journal of Microbiology Research, 3 (2): 066-076.
[18]	Wu PC, Su HJ, and Ho HM (2000). A Comparison of sampling media for environmental viable fungi collected in a hospital environment. Environ. Res. Section A 82: 253-2.
[19]	Nesa D, Lortholary J, Bouakline A, Bordes M, Chandenier J, Derouin F and Gangneux JP (2001). Comparative performance of impactor air samplers for quantification of fungal contamination. J. Hosp. Infect. 47: 149-155.
[20]	Morris G, Kokki MH, Richardson MD (2000). Sampling of Aspergillus spores in air. J. Hosp. Infect. 44: 81-92.
[21]	Gangneux JP, Gangneux FR, Gicquel G, Tanquerel JJ, Chevrier S, Poisson M, Aupee M, Guiguen C (2006). Bacterial and fungal counts in hospital air: comparative yields for 4 sieve impactor air samplers with 2 culture media. Infect Control Hosp Epidemiol. 27: 1405-1 408.
[22]	Prigione V, Lingua G, Marchiosio VF (2004). Development and use of flow cytometry for detection of airborne fungi. Appl. Environ. Microbiol. 70: 1360-1365.
[23]	Gröschel DHM (1980). Air sampling in hospitals. Ann. N. Y. Acad. Sci. 353: 230-240.
[24]	Goodley JM, Clayton YM, Hay RJ (1994). Environmental sampling for aspergilli during building construction on a hospital site. J. Hosp. Infect. 26: 27-35.
[25]	Gerson SL, Parker P, Jacobs MR et al. (et al is not allowed please include all authors name) (1994). Aspergillosis due to carpet contamination. Infect. Control Hosp. Epidemiol. 15: 221-223.
[26]	Pastuszka JS, Marchwinska-Wyrwal E, Wlazlo A (2005). Bacterial aerosol in Silesian hospital: preliminary results. Polish J. Environ. Studies 14 (6): 883-890.
[27]	Tilak, S.T., (1982). Biology of airborne organism: Aerobiology, 1" edn. M.J. Vaidya publishers, pp: 148-258.
[28]	Aneja, K.R., (2007). Experiments in Microbiology, Plant Pathology and Biotechnology. (IV Ed.) New Age International (P) Limited, Publishers. New Delhi. p145-156
[29]	Cuppuccino, J.G. and N. Sherman, (2002). Techniques for isolation of pure culture In. Microbiology: a laboratory manual (VI Ed.) Person Education, Inc. Singapore. p132-133.
[30]	Bhatica L and Vishwakarma R (2010). Hospital Indoor Airborne Microflora in private and Government owned Hospitals in Sagar City, India. World J. Med. Sci. 5 (3): 65-70
[31]	Ekhaise, F.O., Ighosewe O.U. and Ajakpovi O.D. (2008). Hospital Indoor, Airborne Microflora in private and Government Owned Hospital in Benin City, Nigeria. World J. Medical Sci., 3(1): 19-23.
[32]	Kesah, CN, Egri-Okwaji, M.T.C., Odugbemi, T O. and Iroha, EO. (1998). Resistance of Nosocomial Bacterial Pathogens to commonly used Antimicrobial Agents. The Nigerian Postgraduate Medical Journal 6 (2): 60.64.
[33]	Ekhaise F O and Ogboghodo B I (2011) Microbiological Indoor and Outdoor Air Quality of Two Major Hospitals in Benin City, Nigeria. Sierra Leone J Biomed Res 3 (3): 169-174
[34]	Burge, H.A. and M.E. Hoyer, (1990). Indoor air quality. Applied Occupational Environment Hygiene. 5: 84-93.
[35]	Jaffal AA, Nsanze H, Bener A, Ameen AS, Banat IM, El Mogheth AA (1997). Hospital airborne microbial pollution in a desert country. Environ. Internat. 23: 167-172.
[36]	Ehiaghe, F. A., Ehiaghe, I. J., Agbonlahor, D. E., Oviasogie, F. E., Etikerentse, S. M. O., Nwobu, R. A. U., Akinshipe, B. O., Ikusemore, A. I., Aladenika, S. T. and Enwa, F. O. (2013) Plasmid Profiling and Curing Analysis of Fluoroquinolone Multidrug Resistant Pseudomonas aeruginosa in Benin City, Nigeria OJMM, 3: 201-205
[37]	Okhuoya, J.A. and S.O. Okaraedge, 1992. Microflora of road side air and leaf surfaces of selected vegetables. Nigerian Journal of Pure and Applied Science, 12: 42-48.
[38]	Samuel,S. O, Kayode,O. O, Musa,O. I, Nwigwe,G.C, Aboderin A.O, Salami T.A.T and Taiwo S.S (2010). Nosocomial infections and the challenges of control in developing countries. Afr. J. Cln. Exper. Microbiol 11 (2): 102-110
[39]	Ayanru, D.K.G., (1981). Agricultural and clinical significance of some microbial components of the air spores in the Benin-city area. In: Global impact of Applied Microbiology. 6th International Conference. Emejuae, Oguhbi and Sanni (Eds.). Academic Press London, pp: 107-117.
[40]	Jaffal, A.A., I.M. Banat, A.A. El Mogheth, H. Nsanze, A. Benar and A.S. Ameen, 1997. Residential indoor airborne microbial populations in the United Arab Emirates. Environ Internat. 23 (4): 529-533.
[41]	Williams, R.E., O.M. Lldwell and A. Hirch, (1965). The bacterial flora of the air of occupied rooms. Journal of Hygiene, 54: 512-523.
[42]	Prescott, L.M., J.P. Harley and D.A. Klein, 2005. Microbiology. 6th edn. McGraw Hill Co., New York, pp: 92.
[43]	Uneke C. J. and P. A. Ijeoma (2010) The Potential for Nosocomial Infection Transmission by White Coats Used by Physicians in Nigeria: Implications for Improved Patient-Safety Initiatives. World Health Popul. 11 (3): 44-54.
[44]	Kotsans, D., C. Scott, E.E. Gillespie and T.M. Korman. 2008. “What's Hanging around Your Neck? Pathogenic Bacteria on Identity Badges and Lanyards.” Medical Journal of Australia 188 (1): 5-8.
[45]	Loh, W., V.V. Ng and J. Holton. 2000. “Bacterial Flora on the White Coats of Medical Students.” Journal of Hospital Infection 45 (1): 65-8.