On the “All or Half” Law of Recombinant DNA

Gang Zhang^{1, 2,} and Yi Zhang³

¹Department of Medicine, Centre for Research in Neurodegenerative Diseases, University of Toronto, 6 Queen’s Park Crescent West, Toronto, ON, M5S 3H2, Canada

²Department of Cell & Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada

³Program in Life Science, New College, University of Toronto, 40 Willcocks St, Toronto, ON, M5S 1C6, Canada

Cite this paper:
Gang Zhang and Yi Zhang. On the “All or Half” Law of Recombinant DNA. American Journal of Biomedical Research. 2016; 4(1):1-4. doi: 10.12691/ajbr-4-1-1

Abstract

Plasmid vectors are one of the most important tools for the investigation of the functions of genes of interest. Efficient cloning of various vectors, according to different purposes, is critical for biomedical research. Previously, we reported a new method, designated as “Combinatorial Strategy”, for cloning different vectors with various clone sites. We demonstrated that it is a quantitative law for recombinant DNA with our method, which is when two different clone sites are used, almost 100% transformants are positive clones, on the other hand, when one over-hang clone site, or different blunt clone sites are used, about 50% of the transformants are positive clones. We named this quantitative law as the "All or Half" law of recombinant DNA. Here, we summarized the mechanisms of recombinant DNA, provided a general protocol and suggested the predicted results for plasmid vector cloning. This is a revolutionary breakthrough of recombinant DNA technology.

Keywords:
recombinant DNA Combinatorial Strategy "All or Half" law Calf Intestinal Phosphatase site-directed mutagenesis Top10 clone sites

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