World Journal of Agricultural Research
ISSN (Print): 2333-0643 ISSN (Online): 2333-0678 Website: Editor-in-chief: Rener Luciano de Souza Ferraz
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World Journal of Agricultural Research. 2015, 3(5), 148-152
DOI: 10.12691/wjar-3-5-1
Open AccessArticle

Determination of Suitable Agro Climatic Region and Optimum Harvesting Stage by Means of Total Phenolic Content, Total Flavonoid Content and Total Antioxidant Capacity of Ocimum tenuiflorum L. (Lamiaceae) Grown in Sri Lanka

R.M. Dharmadasa1, , K.W. Abeywardhana2, D.C. Abeysinghe2, A.M.L. Aththanayake1, Bandara J3 and V.S. Bandara1

1Industrial Technology Institute, Baudhaloka Mawatha, Colombo, Sri Lanka

2Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP)

3Field Crop Research and Development Center, Mahailluppalama, Sri Lanka

Pub. Date: September 13, 2015

Cite this paper:
R.M. Dharmadasa, K.W. Abeywardhana, D.C. Abeysinghe, A.M.L. Aththanayake, Bandara J and V.S. Bandara. Determination of Suitable Agro Climatic Region and Optimum Harvesting Stage by Means of Total Phenolic Content, Total Flavonoid Content and Total Antioxidant Capacity of Ocimum tenuiflorum L. (Lamiaceae) Grown in Sri Lanka. World Journal of Agricultural Research. 2015; 3(5):148-152. doi: 10.12691/wjar-3-5-1


Ocimum tenuiflorum L. (Lamiaceae) is a industrial important medicinal plant,cultivated for its therapeutic values in many Asian countries. Even though the therapeutic properties of O. tenuiflorum is mainly depend on its secondary metabolites, presently required plant materials for Ayurveda and traditional systems of medicine are collected from the wild without considering its age and therapeutic properties. Therefore, the present study is undertaken tp compare the important phytochemicals (total .phenolic content (TPC), total flavonoid content (TFC)) and total antioxidant capacity (TAC), of different parts of Ocimum tenuiflorum.which were harvested at three consecutive pruning stages from two agro climatic regions in Sri Lanka. TPC, TFC and TAC were performed using colorimetric Folin-Ciocalteu method, aluminum nitrate method and Ferric Reducing Antioxidant Power (FRAP) assay respectively. Data were analyzed using the general linear model (GLM) procedure of Minitab statistical package followed by Duncan’s Multiple Range Test (DMRT) for mean separation. Total antioxidant capacity of leaf, bark, flower and seed extracts were significantly (p=0.05) increased from first pruning to third pruning irrespective of agro climatic regions. Antioxidant capacity and Total flavonoid content of different parts of O. tenuiflorum cultivated in low country intermediate-Zone (IL1a) and low country dry zone (DL1b) was varied as leaf> bark> flower> seeds. Even though the TPC was significantly varied among different parts as well as pruning stages, remarkable relationship was observed neither agro climatic region nor pruning intervals. Results of the present study are vital important for the determination of suitable agroclimatic region, optimum harvesting stage for higher phytochemical, bioactivity and optimum therapeutic properties of O. tenuiflorum.

Ocimum tenuiflorum Lamiaceae total antioxidant capacity total phenoloic content total flavonoid content

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[1]  Ali, H., and Dixit, S, “In-vitro antimicrobial activity of flavonoid of Ocimum sanctum with synergistic effect of their combined form,” Asian Pac J. Trop Dis. 2. 396-398. 2012.
[2]  Singh, E., Sharma, S., Dwivedi, J. and Sharma, S, “Diversified potentials of Ocimum sanctum Linn. (Tulsi): An exhaustive survey,” J. Nat. Prod. Plant Resour; 2(1). 39-48. 2012.
[3]  Abeywardhana, K.W., Abeysinghe, D.C., Dharmadasa, R.M and A.M.L Aththanayake, “Determination of Optimum Maturity Stage for Ocimum sanctum L. Grown under Different Growing Systems in Terms of Therapeutically Active Secondary Metabolites.” World Journal of Agricultural Research, 2(4): 159-162, 2014.
[4]  Dharmadasa, R.M., Premakumara, G.A.S, Hettiarachchi, P.L, Ratnasuriya, W.D. Cytotoxcity and In-vivo antimalarial activity of aqueous whole plant extract of Munronia pinnata (Wall.) Theob. (Meliaceae) in mice. Research Journal of medicinal plant. 6, (3), 267-273, 2012.
[5]  Wang, S. Y.; Lin, H.S. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J. Agric. Food Chem. 48, 140-146. 2000.
[6]  Liu, M, Li, X. Q., Weber, C., Lee, C. Y., Brown, J., and Liu, R. H. Antioxidant and Anti-proliferative Activities of Rasp berries. J. Agric. Food Chem. 2002, 50, 2926-2930. 2002.
[7]  Benzie, I. F. F. and Strain, J.J. The ferric reducing ability of plasma as a measure of antioxidant power: The FRAP assay. Journal of analytical Biochemistry, 293, 70-76. 1996.
[8]  Edreva, A., Velikova, V., Tsonev, T., Dagnon, S., Grel, A., Akta, L., Gesheva, E., Stress-protective role of secondary metabolites: diversity of functions and mechanisms. Gen. Appl. Plant Physiology 34, 67-78. 2008.
[9]  Aysel Sivaci1 and Sevcan Duman, Evaluation of seasonal antioxidant activity and total phenolic compounds in stems and leaves of some almond (Prunus amygdalus L.) varieties. Sivaci and Duman. Biological Research, 47, 2-5, 2014.
[10]  Anttonen, M.J. and R.O. Karjalainen. Environmental and genetic variation of phenolic compounds in red raspberry. Food Composition and Analysis 18,759-769, 2005.
[11]  Ganga Rao. B, Rajeswararao. P, Prayaga Murty. P, Sambasiva Rao. E, Madhukiran.P, Mallikarjuna Rao.T, V.S.Praneeth.D. Investigation on regional variation in total phenolic content, alkaloid content and in-vitro antioxidant activity of Cleome chelidonii L. International Journal of Pharmacy and Pharmaceutical Sciences, 416-418. 2011.
[12]  McGhie , T.K., Martin Hunt , and Laura E. Barnett. Cultivar and growing region determine the antioxidant polyphenolic concentration and composition of apples grown in New Zealand. J. Agric. Food Chem., 53 (8), 3065-3070, 2005.
[13]  Fernando, I. D. N. S., Abeysinghe, D.C. and Dharmadasa, R.M., Determination of phenolic contents and antioxidant capacity of different parts of Withania somnifera (L.) Dunal. from three different growth stages. Industrial Crops and Products, 50, 2013.
[14]  Dharmadasa, R. M., Samarasinghe, K, Adhihetty, P., and Hettiarachchi, P.L. Comparative Pharmacognostic Evaluation ofMunronia Pinnata (Wall.) Theob. (Meliaceae) and its substitute Andrographis paniculata (Burm.f.) Wall. Ex Nees (Acanthaceae). World Journal of Agricultural Research 1(5) 77-81. 2013.
[15]  Thanyaporn Siriwoharn, Ronald E. Wrolstad, Chad E. Finn, And Clifford B. Pereira Influence of cultivar, maturity, and sampling on Blackberry (Rubus L. Hybrids) anthocyanins, polyphenolics, and antioxidant properties. J. Agric. Food Chem., 52, 8021-8030, 2004.