Determination of Cypermethrin, Chlorpyrifos and Diazinon Residues in Tomato and Reduction of Cypermethrin Residues in Tomato Using Rice Bran

M. Alamgir Z. Chowdhury^{1, 2}, Shubhra Bhattacharjee¹, A. N. M. Fakhruddin^1, , M. Nazrul Islam² and M. Khorshed Alam²

¹Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka, Bangladesh

²Agrochemical and Environmental Research Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Bangladesh

Cite this paper:
M. Alamgir Z. Chowdhury, Shubhra Bhattacharjee, A. N. M. Fakhruddin, M. Nazrul Islam and M. Khorshed Alam. Determination of Cypermethrin, Chlorpyrifos and Diazinon Residues in Tomato and Reduction of Cypermethrin Residues in Tomato Using Rice Bran. World Journal of Agricultural Research. 2013; 1(2):30-35. doi: 10.12691/wjar-1-2-2

Abstract

The present study was an attempt to address the health hazard which posed by the ingestation of pesticide residues contained in tomato. Fourteen tomato samples were randomly selected from different shops of local markets of Savar Upazila, Bangladesh and analyzed to identify the level of widely applied cypermethrin, chlorpyrifos and diazinon residues by using high performance liquid chromatography (HPLC) technique. It was found that out of 14 samples, five were found contaminated with cypermethrin and one with chlorpyrifos, but none residue of diazinon was detected in any of the tested samples. Only 7% of the samples were found contaminated with cypermethrin insecticide residue which was above the maximum residue level (MRL) adopted by the FAO/WHO Codex Alimentarius Commission (CAC), although cypermethrin (0.065 ± 0.07mg/kg) and chlorpyrifos (0.024 ± 0.041mg/kg) residues were detected in samples. Rice bran rubbing, a cheap decontamination technique, was used to treat applied cypermethrin residues. 0.45 mg/kg of cypermethrin spiked tomato samples were rubbed by rice bran paste for 5, 10 and 15min. Two experiments were conducted for assessing the efficiency of the adsorbent. Rubbing with rice bran paste for 10min, removed 97.73% and 97.4% in both trails, and whereas cypermethrin residue was not detected in tomato samples treated for 15 min. The removal efficiency was 97.56 ± 0.22% with the adsorbent rice bran rubbing for 10min. The result showed that cypermethrin residues of tomato sample could easily be decontaminated through absorption of rice bran.

Keywords:
solanaceae lycopersicum esculentum pesticides contamination HPLC

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References:

[1]	Ware, G. W. and D. M. Withacre. An introduction to insecticide. 4th Edition. MeisterPro Information Resources, Ohio, USA. pp. 61-69, 2004.
[2]	Chowdhury, B. Vegetables. 6th Edition, The Director, National Book Trust, New Delhi, India. 1979.
[3]	Bose, T. K and M. G. Som. Vegetables Crops in India. 1st Edn. Prokash, Bidan Sarani, Calcutta, India, 1986.
[4]	BBS (Bangladesh Bureau of Statistics). Yearbook of Agricultural Statistics of Bangladesh. Planning Division, Ministry of Planning, Govt. of the People's Republic of Bangladesh, Dhaka, Bangladesh. p.147, 2005.
[5]	Tahir, M. U., S. M., Naik, S. Rehman and M. A. Shahzad. Quantitative Analysis of the Pesticide Residues in Marketed Fruits and Vegetables in Lahore, Pakistan. Biomedical, 25: 171-174, 2009.
[6]	Karim, M. R., M. S. Rahman and M. S. Alam. 2000. Profitability of Summer BARI Hybrid Tomato Cultivation in Jessore District of Bangladesh. J. Agric. Rural. Dev., 7: (1&2), 73-79, 2000.
[7]	Parveen, S. and N. Nakagoshi. An Analysis of Pesticide Use for Rice Pest Management in Bangladesh. J. Int. Dev. Coop., 8 (7): 107-111, 2001.
[8]	Kalam, K. M. H, A. Bangladesh Country Paper. In: Environmental Assessment for Agricultural Development in Asia and the Pacific. Asian Productivity Organization (APO), Tokyo. Japan. pp. 141-152, 1998.
[9]	PAB (Pesticide Association of Bangladesh). Pesticides Consumption Report for the Year 1999/2000. Pesticide Association of Bangladesh, Dhaka. pp. 18-19, 2000.
[10]	McCauley, L. A., W. K. Anger, M. Keifer, R. Langley,M. G. Robson and D. Rohlman. Studying Health Outcomes in Farm Worker Populations Exposed to Pesticides. Environ. Health Pers., 114 (3): 953-960, 2006.
[11]	Ali, R. B. and A. Tahir. Preliminary Survey for Pesticide Poisoning in Pakistan. Pakistan J. Biol. Sci., 3 (11): 1976-1977, 2000.
[12]	Rahman, M. H. and M. J. B. Alam. 1997. Risk Assessment of Pesticide Use in Bangladesh. J. Civi.l Eng., 25: 97-102, 1997.
[13]	Meisner, C. Reports of Pesticide Hotspots in Bangladesh. Available athttp://siteresources.worldbank.org/INTEAER/Resources/59916061251483570919/Report_of_Pesticide_Hotspots_in_Bangladesh.pdf. 2004.
[14]	Koirala, P., D. B. Khadka and A. Mishra. Pesticide Residues as Environmental Contaminants in Foods in Nepal. J. Agric. Environ., 8: 96-100, 2007.
[15]	Adachi, A and T. Okano. Pesticide residues reducation in selected vegetables using rice bran. J Health Sci., 52 (3): 320-323, 2006.
[16]	Aysal, P., K. Gozek, N. Artik and A. S. Tunebilek. 14C-Chlorpyrifos Residues in Tomatoes and Tomato Products. Bull. Environ. Cont. Toxicol., 62(4), 377-382, 1999.
[17]	CAC (CODEX Alimentarius Commission). Pesticide Residues in Food and Feed. Available athttp://www.codexalimentarius.net/pestres/data/pesticides/details.html?id=22. 2010.
[18]	TAS (Thai Agricultural Standard). Pesticide Residues: Maximum Residue Limits. National Bureau of Agricultural Commodity and Food Standards, Ministry of Agriculture and Cooperatives. Published in the Royal Gazette l (125 Special Section 139D). p.10, 2008.
[19]	Morasso, G., C. Bolognesi, E. Duglio and M. Musso. Pesticides as food contaminants: a pilot project for correct public information. Trends Food. Sci. Technol., 11: 379-82, 2001.
[20]	Khatoon, J. A., M. S. Islam, M. N. Talukdar and M. A. Hossain. Monitoring of the Residue Level of Three Selected Pesticide in Red Amaranth. Pakistan J. Biol. Sci., 4 (4), 474-479, 2004.
[21]	Fenske, R. A., G. Kedan, C. Lu,J. A. Fisker-Andersen and C. L. Curl. Assessment of Organophosphorous Pesticide Exposures in the Diets of Pre-school Children in Washington State. J. Exposure Anal. Environ. Epidemiol., 12: 21-28, 2002.
[22]	Chowdhury, M. T. I., M. A. Razzaque and M. S. I. Khan. Chlorinated Pesticide Residue Status in Tomato, Potato and Carrot. J. Exp. Sci. 2 (1): 1-5, 2011.
[23]	Fenoll, J., P. Hellin, C. Martinez, M. Miguel and P. Flores. Multiresidue Method for Analysis of Pesticides in Pepper and Tomato by Gas Chromatography with Nitrogen–Phosphorus Detection. Food Chem., 105: 711-719, 2006.
[24]	Karnath, N. G. K. Challenges of Limiting Pesticide Residues in Fresh Vegetables: the Indian Experience. Food Safety Management in Developing Countries. Proceeding of International Workshop, CIRAD-FAO. pp. 1-13, 2002.
[25]	Kumar, D, R. C. Sharma and P. Chauhan, P. Estimation of Multiclass Pesticide Residues in Tomato (Lycopersicon esculentum) and Radish (Raphanus sativus) Vegetables by Chromatographic Methods. Res J Agric Sci., 2 (1): 40-43, 2011.
[26]	Adachi, A., S.Takagi and T. Okano. Studies on removal efficiency of rice bran for pesticides. J. Health Sci., 47 (2): 94-98, 2000.
[27]	Adachi, A., C. Ikeda, S. Takagi, N. Fukao, E. Yoshii and T Okano. 2001. Efficiency of rice bran for removal of Organochlorine Compounds and benzene from industrial wastewater. J. Agric. Food Chem., 48: 1309-1314, 2001.
[28]	Buttrose, M. S. and C. Ikeda. Ultrastructure of the developing aleurone cells of wheat grains. J. Biol. Sci., 16: 768-774, 1963.
[29]	Adachi, A., Y. Okita and J. Adachi. Efficiency of rice bran for removal of Pretilachlor and Esprocarb in Artificial Gastric Fluid. Organochlorine Compounds and benzene from industrial wastewater. J. Health Sci., 56 (1): 88-91, 2009.
[30]	Kesarwani, R., S. Debnath, S. Kshatri and S. P. Rajiv. Use of rice bran for adsorption of color based effluents. Chem. Ind. Des., 13: 97-100, 2000.
[31]	Hosseinnia, A., M. S. Hashtroudi, M. Pazouki and M Banifatemi. Removal of surfactant from wastewater by rice husk. Iranian J. Chem. Eng., 3 (3): 44-49, 2006.