Evaluation of Molecular and Serological Diagnostic Techniques for a Large Scale Detection of Plum Pox Virus

Jifara Teshale^1,

¹Ambo University, College of Agriculture, Department of Agribusiness and value chain management, Ambo, Ethiopia

Cite this paper:
Jifara Teshale. Evaluation of Molecular and Serological Diagnostic Techniques for a Large Scale Detection of Plum Pox Virus. Research in Plant Sciences. 2014; 2(2):33-41. doi: 10.12691/plant-2-2-1

Abstract

Plum pox virus (PPV), the agent of sharka, is the most devastating virus infecting stone fruits. The PPV control is mainly based on prevention, and its quick and reliable detection is considered crucial in this strategy. In this study DAS-ELISA and Real-time PCR were compared for evaluating their potentialities and limits for large scale surveys. Different hosts (apricot and peach), plant organs (phloem, buds, flowers, leaves and fruits) and parts of them, different periods of the year, the presence or absence of symptoms were considered for comparison. No significant differences were observed between the two techniques when the titre of the virus in the plant is high (on spring). Conversely, Real-time is preferable to ELISA for testing tissues with low virus concentration (in the early stages of infection or during summer). Moreover, the accurate and careful observation of symptoms in the field remains crucial in the application of the disease monitoring and eradication programs.

Keywords:
plum pox virus Real time-PCR DAS-ELISA stone fruits

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References:

[1]	Boscia, D., Zeramdini, H., Cambra, M., Potere, O., Gorris, M.T. and Myrta, A. 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. Eur. J. Plant Pathol. 103: 447-480.
[2]	Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J.E. 1996. Plum pox potyvirus. Plant Viruses Online: Descriptions and Lists from the VIDE Database. http://image.fs.uidaho.edu/vide/descr630.htm
[3]	Candresse T., Cambra M., Dallot S., Lanneau M., Asensio M., Gorris M.T., Revers F., Macquaire G., Olmos A, Boscia D., Quiot J. B. and Dunez J. (1998). Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of Plum pox potyvirus. Phytopathology, n. 88: 198-20.
[4]	Cambra, M., Asensio, M., Gorris, M.T., Pérez, E., Camarasa, E., García, J.A., López-Moya, J.J., López-Abella, D., Vela, C. and Sanz, A. 1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non structural proteins. OEPP/EPPO Bulletin 24: 569-577.
[5]	Candresse, T. and Cambra, M. 2006. Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains. OEPP/EPPO Bulletin 36: 239-246.
[6]	Capote N. Gorris M.T. Martínez M.C. Asensio M. Olmos A. and Cambra M. (2006). Interference between D and M types of Plum pox virus. Japanese plums assessed by specific monoclonal antibodies and quantitative real-time RT-PCR. Phytopathology, n. 96: 320-325.
[7]	Capote, N., Bertolini, E., Olmos, A., Vidal, E., Martínez, M.C., Cambra, M. 2009. Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR. Int. Microbiol. 12: 1-6.
[8]	Christoff A. (1934). Mosaikkrankheit oder Viruschlorose, bei Apfeln. Eine neus Viruskrankheit. Phytopathology, n. 7: 521-536.
[9]	Clark M.F. and Adams A.N. (1977). Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, n. 34: 475-483.
[10]	Dunez J. and Sutic D. (1988). Plum Pox Virus. In: Smith I.M., Dunez J., Elliot R.A., Phillips D.H., and S. A. Arches S.A. (Eds). European Handbook of Plant Diseases. Blackwell, London. pp. 44-6.
[11]	Gentit, P., 2006. Detection of Plum pox virus: biological methods. OEPP/EPPO Bulletin 36: 251-253.
[12]	James, D. and Thompson, D. 2006. Hosts and symptoms of Plum pox virus: ornamental and wild Prunus species. OEPP/EPPO Bulletin 36: 222-224.
[13]	Levy, L. and Hadidi, A. 1994. A simple and rapid method for processing tissue infected with plum pox potyvirus for use with specific-3’non-coding region RT-PCR assays. OEPP/EPPO Bulletin 24: 595-604.
[14]	López-Moya J.J., Fernández-Fernández M.R., Cambra M. and García J.A. (2000). Biotechnological aspects of plum pox virus. Journal of Biotechnology, n. 76: 121-136.
[15]	Myrta, A., Potere, O., Boscia, D., Candresse, T., Cambra, M. and Savino, V. 1998. Production of a monoclonal antibody specific to the El Amar strain of plum pox virus. Acta Virol. 42: 248-250.
[16]	Myrta, A., Potere, O., Crescenzi, A., Nuzzaci, M. and Boscia, D. 2000. Production of two monoclonal antibodies specific to cherry strain of plum pox virus (PPV-C). J. Plant Pathol. 82: 95-103.
[17]	Nemchinov, L., Hadidi, A., Maiss, H., Cambra, M., Candresse, T. and Damsteegt, V. 1996. Sour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence of a new subgroup of PPV strains. Phytopathology 86: 1215-1221.
[18]	Németh M. (1986) Virus diseases of stone fruit trees. Virus, Mycoplasma and Rickettsia Diseases of Fruit Trees, pp. 256-545.
[19]	Nemeth M. (1994). History and importance of plum pox virus in stone fruit production. OEPP/EPPO Bulletin, n. 24: 525-536.
[20]	Olmos, A., Bertolini, E., Gil, M. and Cambra, M. 2005. Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. J. Virol. Methods 128: 151-155.
[21]	Salvador, B., García, J.A., Simόn-Mateo, C. 2006. Causal agent of sharka disease: Plum pox virus genome and function of gene products. OEPP/EPPO Bulletin 36: 229-238.
[22]	Serçe, C.U., Candresse, T., Svanella-Dumas, L., Krizbai, L., Gazel, M., Cağlayan, K. 2009. Further characterization of a recombinant group of Plum pox virus isolates, PPV-T, found in orchards in Ankara province of Turkey. Virus Res. 142: 121-126.
[23]	Schneider, W.L., Sherman, D.J., Stone, A.L., Damsteegt, V.D. and Frederick, R.D. 2004. Specific detection and quantification of Plum pox virus by real-time fluorescent reverse transcription-PCR. J. Virol. Methods 120: 97-105.
[24]	Varga, A. and James, D. 2005. Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing. J. Virol. Methods 123: 213-220.
[25]	Varveri, C., Candresse, T., Cugusi, M., Ravelonandro, M. and Dunez, J. 1988. Use of a 32P labelled transcribed RNA probe for dot hybridization detection of plum pox virus. Phytopathology 78: 1280-1283.
[26]	Wetzel, T., Candresse, T., Macquaire, G., Ravelonandro, M. and Dunez, J. 1992. A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. J. Virol. Methods 39: 27-37.
[27]	Wetzel, T., Candresse, T., Ravelonandro, M. and Dunez, J. 1991. A polymerase chain reaction assay adapted to plum pox virus detection. J. Virol. Methods 33: 355-365.
[28]	Wetzel, T., Tavert, G., Teycheney, P.Y., Ravelonandro, M., Candresse, T. and Dunez, J. 1990. Dot hybridisation detection of plum pox virus using 32 P-labeled RNA probes representing non-structural viral protein genes. J Virol. Methods 30: 161-171.