Evaluation of the Effect of the Aqueous Extract of the Recipe of the Leaves of O. Grastissium and the Steam Bark of T. Superba on Acetic Acid-Induced Spasms in Wistar Rats

Boukongo R. P.¹, Elion Itou RDG^{1, 2,} , Mambeke H. M.¹, Etou Ossibi AW^{1, 3}, Morabandza C. J.⁴ and Abena AA⁵

¹Laboratory of Pharmacodynamics and Experimental Pathophysiology (L2PE), Marien University Ngouabi, BP69, Brazzaville, Congo

²National Institute for Research in Health Sciences, Scientific City of Brazzaville (formerly ORSTOM), Route de l’Auberge de Gascogne (chateau d’eau), Congo;Laboratory of Biochemistry and Pharmacology, Faculty of Health Sciences, University Marien Ngouabi, BP 69, Brazzaville, Congo

³National Institute for Research in Health Sciences, Scientific City of Brazzaville (formerly ORSTOM), Route de l’Auberge de Gascogne (chateau d’eau), Congo

⁴Department of Natural Sciences, Ecole Normale Supérieure, Marien University, Ngouabi Congo, B.P 69, Brazzaville, Congo

⁵Laboratory of Biochemistry and Pharmacology, Faculty of Health Sciences, University Marien Ngouabi, BP 69, Brazzaville, Congo

Cite this paper:
Boukongo R. P., Elion Itou RDG, Mambeke H. M., Etou Ossibi AW, Morabandza C. J. and Abena AA. Evaluation of the Effect of the Aqueous Extract of the Recipe of the Leaves of O. Grastissium and the Steam Bark of T. Superba on Acetic Acid-Induced Spasms in Wistar Rats. Research in Plant Sciences. 2023; 11(1):22-28. doi: 10.12691/plant-11-1-4

Abstract

Intestinal spasms are violent contractions that occur in the intestine. Aside from treatment with conventional medications, certain medicinal plants are widely used alone or in combination in traditional congolese medicine to treat spasms. This study aims to evaluate the antispasmodic effects of an aqueous extract of the recipe made from the leaves of O. gratissimum and the bark of T. superba. The spasms were induced 1 hour after administration of the aqueous extract by intraperitoneal injection of 1% acetic acid; the duration of the experiment varied from 30, 60 and 90 minutes. Then, the animals were sacrificed after anesthesia with ethyl ether, the peritoneum was rinsed with 0.9% Nacl, the peritoneal fluid obtained in order to measure prostaglandins (PGE2α and PGF2α), as well as oxidative stress parameters. The aqueous extract at the doses (125,250 and 500 mg/kg) used has an antispasmodic effect which resulted in a significant reduction (p<0.001) in the number of spasms. The antispasmodic effects were confirmed by a significant decrease (p<0.001) in prostaglandins PGE2α and PGF2 for their involvement in the pain induction mechanism of acetic acid. Furthermore, a significant reduction (p<0.001) in the parameters of oxidative stress induced by the intraperitoneal injection of acetic acid was observed. The results showed that the aqueous extract of the recipe has significant antispasmodic activity and could justify the traditional use of the recipe in abdominal pain.

Keywords:
spasms antispasmodic O. gratissimum T. superba recipe

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