Effect of the Electric Field on the Antibacterial Activity of Au Nanoparticles on Some Gram-positive and Gram-negative Bacteria

Thamir Jumaa¹, Maysaa Chasib^2, , Mazin K. Hamid¹ and Raad Al-Haddad³

¹Department of Physics, College of Sciences, AL-Nahrain University, Baghdad, Iraq

²Department of Biotechnology, College of Sciences, AL-Nahrain University, Baghdad, Iraq

³Department of Physics, College of Sciences, Baghdad University, Baghdad, Iraq

Cite this paper:
Thamir Jumaa, Maysaa Chasib, Mazin K. Hamid and Raad Al-Haddad. Effect of the Electric Field on the Antibacterial Activity of Au Nanoparticles on Some Gram-positive and Gram-negative Bacteria. Nanoscience and Nanotechnology Research. 2014; 2(1):1-7. doi: 10.12691/nnr-2-1-1

Abstract

Metal nanoparticles are being extensively used in various biomedical applications due to their small size to volume ratio and extensive thermal stability. Gold nanoparticles (AuNPs) are an obvious choice due to their amenability of synthesis and functionalization, less toxicity and ease of detection. The synthesis and bioactivity of gold nanoparticles has been extensively studied. The present study was focused on method to increase the activity and the efficacy of the antibacterial activity of gold nanoparticles which produced by laser ablation of 1064 nm wavelength and three energy powers (400,500,600) mJ were applied to produced gold nanoparticles with different sizes on Gram-positive isolate (Staphylococcus aureus) and the Gram-negative isolate(Pseudomonas aeroginosa). It was found that using the agar well diffusion assay method which showed that the individually of AuNPs of 0.2 mg/ml concentration have no synergistic effect on the studied Staphylococcus aureus and Pseudomonas. So, a new modified technique was made on these AuNPs with the same concentration to increase their antibacterial activity, is exposure the gold nanoparticles colloidal to 1500 v/m applied electric field which resulting to be an effective AuNPs with inhibition properties against Gram-positive isolates (Staphylococcus aureus) contrary to nanoparticles that was not exposed to electric field with the same concentration.

Keywords:
gold nanoparticles Mie scattering antibacterial activity Staphylococcus aureus Pseudomonas aeruginosa electric field well diffusion

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