Exopolysaccharides Produced by Rhizobium: Production, Composition and Rheological Properties

Ribeiro V.A.^1, and Burkert C.A.V.¹

¹Federal University of Rio Grande, School of Chemistry and Food, Rio Grande, RS, Brazil

Cite this paper:
Ribeiro V.A. and Burkert C.A.V.. Exopolysaccharides Produced by Rhizobium: Production, Composition and Rheological Properties. Journal of Polymer and Biopolymer Physics Chemistry. 2016; 4(1):1-6. doi: 10.12691/jpbpc-4-1-1

Abstract

The use of exopolysaccharides (EPS) in industrial product formulations has increased in recent years due to their ability to increase the viscosity of solutions or cause the formation of gels, affecting the texture of products. In industry, EPS can be added as gelling, thickening and stabilizing agents in foods, pharmaceuticals and cosmetics. In this context, EPS from nitrogen-fixing rhizobial bacteria are emerging as potential biopolymers for industrial applications. However, the establishment of cultivation conditions, their chemical structure and physicochemical characteristics, such as rheological behavior, are essential to enable their use on a large scale. Furthermore, the possibility of using byproducts and agroindustrial wastes as substrates can contribute to the economic feasiability of the process. In this context, this article aims to present a review of EPS synthesized by different strains of Rhizobium in relation to their production, composition and rheological properties.

Keywords:
fixing-nitrogen bacteria rhizobia biopolymers extracellular polysaccharides rheological behavior

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References:

[1]	Aranda-Selverio, G., Penna, A.L.B., Campos-Sás, L.F., Santos Junior, O., Vasconcelos, A.F.D., Silva, M.L.C., Lemos, E.G.M., Campanharo, J.C., Silveira, J.L.M., “Propriedades reológicas e efeito da adição de sal na viscosidade de exopolissacarídeos produzidos por bactérias do gênero Rhizobium”, Química. Nova, 33(4). 895-899. March 2010.
[2]	Barbosa, A.M., Cunha, P.D.T., Pigatto, M.M., Silva, M.L.C., “Produção e aplicações de exopolissacarídeos fúngicos”, Semina: Ciências Exatas e Tecnológicas, 25(1). 29-42. January/June 2004.
[3]	Barreto, M.C.S., Figueiredo, M.V.B., Burity, H.A., Silva, M.L.R.B., Lima-Filho, J.L., “Produção e comportamento reológico de biopolímeros produzidos por rizóbios e caracterização genética”, Revista Brasileira de Agrociência, 17(2-4). 221-227. April/June 2011.
[4]	Becker, A., Pühler, A., Production of exopolysaccharides, Kluwer Academic Publishers, Oegstgeest, 1998, 97-118.
[5]	Breedveld, M.W., Zevenhuizen, L.P.T.M., Zehnder, A.J.B., “Osmotically induced oligo- and polysaccharide synthesis by Rhizobium meliloti SU-47”, Journal of General Microbiology, 136. 2511-2519. August 1990.
[6]	Berrada, H., Fikri-Benbrahim, K., “Taxonomy of the Rhizobia: current perspectives”, British Microbiology Research Journal, 4(6). 616-639. June 2014.
[7]	Bomfeti, C.A., Florentino, L.A., Guimarães, A.P., Cardoso, P.G., Guerreiro, M.C., Moreira, F.M.S., “Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae”, Revista Brasileira de Ciência do Solo, 35(3). 657-671. May/June 2011.
[8]	Castellane, T.C.L., Lemos, E.G.M., “Composição de exopolissacarídeos produzidos por estirpes de rizóbios cultivados em diferentes fontes de carbono”, Pesquisa Agropecuária Brasileira, 42(10). 1503-1506. October 2007.
[9]	Castellane, T.C.L., Lemos, M.V.F., Lemos, E.G.M., “Evaluation of the biotechnological potential of Rhizobium tropici strains for exopolysaccharide production”, Carbohydrate Polymers, 111(13). 191-197. October 2014.
[10]	Castellane, T.C.L., Persona, M.R., Campanharo, J.C., Lemos, E.G.M., “Production of exopolysaccharide from rhizobia with potential biotechnological and bioremediation applications”, International Journal of Biological Macromolecules, 74. 515-522. March 2015.
[11]	Devi, S.E, Vijayendra, S.V.N., Shamala, T.R., “Exploration of rice bran, an agroindustry residue, for the production of intra- and extra-cellular polymers by Sinorhizobium meliloti MTCC 100”, Biocatalysis and Agricultural Biotechnology, 1(1). 80-84. January 2012.
[12]	Diaz, P.S., Vendruscolo, C.T., Vendruscolo, J.L.S., “Xanthan rheological: a review about the influence of electrolytes on the viscosity of aqueous solutions of xanthan gums”, Semina: Ciências Exatas e Tecnológicas, 25(1). 15-28. June 2004.
[13]	Donot, F., Fontana, A., Baccou, J.C., Schorr-Galindo, S., “Microbial exopolysaccharides: main examples of synthesis, excretion, genetics and extraction”, Carbohydrate Polymers, 87(2). 951-962. January 2012.
[14]	Duta, F.P., França, F.P., Lopes, L.M.A., “Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method”, Electronic Journal of Biotechnology, 9(4). 391-399. July 2006.
[15]	Fernandes Júnior, P.I., Almeida, J.P.S., Passos, S.R., Oliveira, P.J., Rumjanek, N.G., Xavier, G.R., “Produção e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu”, Pesquisa Agropecuária Brasileira, 45(12). 1465-1471. December 2010.
[16]	Freitas, F., Alves, V.D, Reis M.A.M., “Advances in bacterial exopolysaccharides: from production to biotechnological applications”, Trends in Biotechnology, 29(8). 388-398. August 2011.
[17]	Ghosh, A.C., Ghosh, S., Basu, P.S., “Production of extracellular polysaccharide by a Rhizobium species from root nodules of the leguminous tree Dalbergia lanceolaria”, Engineering in Life Sciences, 5(4). 378–382. August 2005.
[18]	Guentas, L., Pheulpin, P., Michaud, P., Heyraud, A., Gey, C., Courtois, B., Courtois, J., “Structure of a polysaccharide from a Rhizobium species containing 2-deoxy--D-arabino-hexuronic acid”, Carbohydrate Research, 332 (2). 167-173. May 2001.
[19]	Janczarek, M., Rachwał, K., Cieśla, J., Ginalska, G., Bieganowski, A., “Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties”, Plant Soil, 388. 211-227. March 2015.
[20]	Kaci, Y., Heyraud, A., Barakat, M., Heulin, T., “Isolation and identification of an EPS producing Rhizobium strain from arid soil (Algeria): characterization of its EPS and the effect of inoculation on wheat rhizosphere soil structure”, Research in Microbiology 156(4). 522-531. May 2005.
[21]	Kayacier, A., Dogan, M., “Rheological properties of some gums-salep mixed solutions”, Journal of Food Engineering, 77(3). 261-265. February 2006.
[22]	Kumar, A.S., Mody, K., Jha, B., “Bacterial exopolysaccharides: a perception”, Journal of Basic Microbiology, 47(2). 103-117. April 2007.
[23]	Kutkowska, J., Tuska-Szewczuk, A., Janczarek, M., Paduch, R., Kaminska, T., Urbanik-Sypniewska, T., “Biological activity of (lipo)polysaccharides of the exopolysaccharide-deficient mutant Rt120 derived from Rhizobium leguminosarum bv. trifolli strain TA2”, Biochemistry (Moscow), 76(7). 840-850. July 2011.
[24]	Li, S., Huang, R., Shah, N.P., Tao, X., Xiong, Y., Wei, H., “Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315”, Journal of Dairy Science, 97(12). 7334-7343. December 2014.
[25]	Liu, Y., Gu, O., Ofosu, F.K., Yu, X., “Production, structural characterization and gel forming property of a new exopolysaccharide produced by Agrobacterium HX1126 using glycerol or D-mannitol as substrate”, Carbohydrate Polymers, 136(20). 917-922. January 2016.
[26]	Mandal, S., Ray, B., Dey, S., Pati, B., “Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L) Hepper”, Biotechnology Letters, 29(8). 1271-1275. August 2007.
[27]	Mesomo, M.C., Silva, M.F., Boni, G., Padilha, F.F., Mazutti, M., Mossi, A., Oliveira, D., Cansian, R.L., Luccio, M.D., Treichel, H., “Xanthan gum produced by Xanthomonas campestris from cheese whey: production optimisation and rheological characterization”, Journal of the Science of Food and Agriculture, 89(14). 2440-2445. November 2009.
[28]	Monteiro, N.K., Aranda-Selverio, G., Exposti, D.T.D., Silva, M.L.C., Lemos, E.G.M., Campanharo, J.C., Silveira, J.L.M., “Caracterização química dos géis produzidos pelas bactérias diazotróficas Rhizobium tropici e Mesorhizobium sp.”, Química Nova, 35(4). 705-708. January 2012.
[29]	Moreira, F.M.S., Siqueira, J.O., Microbiologia e bioquímica do solo, Editora da UFLA, Lavras, 2006, 729 p.
[30]	Moretto, C., Castellane, T.C.L., Lopes, E.M., Omori, W.P., Sacco, L.P., Lemos, E.G.M., “Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia”, International Journal of Biological Macromolecules, 81. 291-298. July 2015.
[31]	Navarini, L., Stredansky, M., Matulova, M., Bertocchi, C., “Production and characterization of an exopolysaccharide from Rhizobium hedysari HCNT 1”, Biotechnology Letters, 19(12). 1231-1234. December 1997.
[32]	Pasquel, A., “Gomas: utilização e aspectos reológicos”, Boletim da Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 33(1). 86-87. 1999.
[33]	Prasanna, P.H.P., Bell, A., Grandison, A.S., Charalampopoulos, D., “Emulsifying, rheological and physicochemical properties of exopolysaccharide produced by Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205”, Carbohydrate Polymers, 90. 533-540. September 2012.
[34]	Priyanka, P., Arun, A.B., Ashwini, P., Rekha. P.D., “Versatile properties of an exopolysaccharide R-PS18 produced by Rhizobium sp. PRIM-18”, Carbohydrate Polymers, 126. 215-221. August 2015.
[35]	Ramalingam, C., Priya, J., Mundra, S., “Applications of microbial polysaccharides in food industry”, International Journal of Pharmaceutical Sciences Review and Research, 27(1). 322-324. July/August 2014.
[36]	Ribeiro, V.A., Teixeira, M., Burkert, C.A.V., “Cultivo de bactérias utilizando glicerina residual para obtenção de exopolissacarídeos”, in XX Congresso Brasileiro de Engenharia Química (COBEQ), Associação Brasileira de Engenharia Química.
[37]	Sellami, M., Oszako, T, Miled, N., Rebah, F.B., “Industrial wastewater as raw material for exopolysaccharide production by Rhizobium leguminosarum”, Brazilian Journal of Microbiology, 46(2). 407-413. June 2015.
[38]	Skorupska, A., Janczarek, M., Marczak, M., Mazur, A., Król, J., “Rhizobial exopolysaccharides: genetic control and symbiotic functions”, Microbial Cell Factories, 5(7). 1-19. February 2006.
[39]	Staehelin, C., Forsberg, L.S., D'Haeze, W., Gao, M.Y., Carlson, R.W., Xie, Z.P., Pellock, B.J., Jones, K.M., Walker, G.C, Streit, W.R., Broughton, W.J., “Exo-oligosaccharides of Rhizobium sp. strain NGR234 are required for symbiosis with various legumes”, Journal of Bacteriology, 188(17). 6168-6178. September 2006.
[40]	Staudt, A.K., Wolfe, L.G., Shrout, J.D., “Variations in exopolysaccharide production by Rhizobium tropici”, Archives of Microbiology, 194(3). 197-206. March 2012.
[41]	Toneli, J.T.C.L., Murr, F.E.X., Park, K.J., “Estudo da reologia de polissacarídeos utilizados na indústria de alimentos”, Revista Brasileira de Produtos Agroindustriais, 7(2). 181-204. 2005.
[42]	Willems, A., “The taxonomy of rhizobia: an overview”, Plant and Soil, 287(1). 3-14. September 2006.
[43]	Zhou, R., Wu, Z., Chen, C., Han, J., Ai, L., Guo, B., “Exopolysaccharides produced by Rhizobium radiobacter S10 in whey and their rheological properties”, Food Hydrocolloids 36. 362-368. May 2014.