Amino Acid and Heavy Metal Composition of Afzelia africana Leaves

Umedum Ngozi Lillian^1, , Nwosu Christian Chisom¹, Udeozo Ifeoma Prisca² and Igwemmar Noela Chinyere³

¹Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria

²Department of Chemistry, Tansian University, Oba, Nigeria

³Department of Chemistry, University of Abuja, Gwagwalada, Nigeria

Cite this paper:
Umedum Ngozi Lillian, Nwosu Christian Chisom, Udeozo Ifeoma Prisca and Igwemmar Noela Chinyere. Amino Acid and Heavy Metal Composition of Afzelia africana Leaves. World Journal of Nutrition and Health. 2014; 2(2):17-20. doi: 10.12691/jnh-2-2-1

Abstract

Malnutrition is one of the major problems in Nigeria and other developing countries. The country is blessed with rich soil and favorable climate, but lack of irrigation facilities has resulted to seasonality; and ignorance of nutritional value of lesser-known vegetables have contributed to scarcity of vegetables for the increasing population. This study investigated the amino acid and heavy metal composition of Afzelia africana leaves. Sixteen amino acids were found in varying proportions in the protein of the vegetable. All essential amino acids, except methionine and tryptophan were present in good quantities. Phenylalanine had the highest concentration of 2.27g/100g protein while isoleucine had the lowest concentration of 1.71g/ 100g protein. The heavy metal analysis was done by atomic absorption spectrometry. Results obtained showed the presence of the essential metals: iron (8.49ppm) and nickel (0.03ppm); and the non-essential metals: lead (0.75ppm) and mercury (0.64ppm). Results revealed that the protein contained in the leaves of Afzelia africana is of high quality; however the presence of the toxic metals is an issue of great concern.

Keywords:
Afzelia Africana amino acids vegetable heavy metals FAO/ WHO limit

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References:

[1]	World Food Programme. Food and Nutrition Handbook. Rome: World Food Programme, 2000.
[2]	FAO Food and Agriculture Organization of the United Nations. News release:Global hunger declining, but still unacceptably high. Rome, Italy, 2010.
[3]	WHO World Health Organization. News release” New online nutrition initiative can help protect lives and health of millions of children”. 2011. Available from http://www.who.int/mediacentre/news/releases/2011/nutrition_20110810/en/ accessed 8/3/2013
[4]	Obinna C. “Malnutrition: Great concern, little action in Nigeria”. Vanguard News, December 2012.
[5]	Bain L.E., Awah P.,K., Ngia G., Kindong N.P.,Sigal Y., Bernard N., Tanjeko A.T.,”Malnutrition in Sub-saharan Africa: burden, causes and prospects”. The Pan African Medical Journal, 15(120), 2013.
[6]	Ezzati F., Lopez A.D., Rodgers A., Hoorn S.V., Murray C.J., “Selected major risk factors and global and regional burden of disease”. The Lancet, 360(9343), 1347-1360, 2002.
[7]	Igwenyi I.O., Offor C.E., Aja P.M, Aloh G.S., Orji O.U., and Afiukwa C.A., “Potentials of Afzelia africana vegetable oil in biodiesel production”. Asian Journal of Biochemistry 6(6), 450-457, 2011.
[8]	Mefoh N.C., Obizoba I.C., Madukwe E.U., “Chemical, nutritive value and organoleptic attributes of in-built products of fermented Afzelia africana tender leaves and shoots”. International Journal of Scientific and Research Publications 3(8), 1-8, August 2013.
[9]	Ikhimioya I., Bamikole M.A, Omoregie A.U, and Ikhatua U.J., “Compositional evaluation of some dry season shrub and tree foliages in a transitionally vegetated zone of Nigeria”. Livestock Research for Rural Development 19(3), March, 2007.
[10]	Igbabul B., Hiikyaa O, Amove J. “Effect of fermentation on the proximate composition and functional properties of mahogany bean (Afzelia africana) flour”. Curr Res Nutr.Food Sci 2(1), 2014. Available from http://www.foodandnutritionjournal.org/?P=611.
[11]	Orwa C., Metua A., Kindt R., Jamnadass R., and Simons A., Agroforestree database: a tree reference and selection guide version 4.0, 2009. Available from http://www.worldagroforestry.org/af/treedb/.
[12]	Oduguwa O.O., Ikeobi C.O.N., Oduguwa B.O., and Oyedele O.O., “Chemical evaluation of foliage of some tropical leguminous trees and shrubs as fodder”. Pertanika J. Trop. Agric. Sci 20(1), 31-34, 1997.
[13]	Igwenyi I.O and Akubugwo E.I., “Analysis of four seeds used as soup thickeners in the South-eastern part of Nigeria”. Proceedings of the International conference on Chemistry and Chemical Engineering, 426-430, 2010.
[14]	Sparkman D.H.,Stein E.H., Moore S., Automatic recording apparatus for use in chromatography of amino acids. Anal. Chem 30, 119, 1958.
[15]	Okalebo J.R.,Catha K.W., and Woomer P.L. 2002 Laboratory methods of s oil and plant analysis: a working manual TSBF-CIAT and SACRED Africa, Nairobi, Kenya 200.
[16]	White J. Essential amino acids and their functions available from http://how-to-burn-fat.com/nutrition/essential-amino-acids-and-their-functions accessed 8/3/2014.
[17]	Kubmarawa D., Andenyang I.F.H and Magomya A.M 2009 Proximate composition and amino acid profile of two non-conventional leafy vegetables (Hibiscus cannabinus and Haematostaphis barteri). African Journal of Food Science, 3(9), 233-236, 2009.
[18]	Kubmarawa D., Shangal M.H., and Diwu B.G., “Amino acid profile of Amaranthus caudatus”. E3 Journal of Biotechnology and Pharmaceutical Research, 4(4), 68-72, 2013. Available from http://www.e3journals.org/JBPR.
[19]	Fuh C.,Lin H. And Tsai H., “Determination of lead, cadmium and arsenic in 13 herbs of tocolysis formulation using atomic absorption spectrometry”. Journal of Food and Drug Analysis, 11(1), 39-45, 2003.
[20]	WHO. Cadmium environmental health criteria. World Health Organization, Geneva, Switzerland, p 134, 1992.
[21]	FAO/WHO, Contaminants. In Codex Alimentarius, vol xvII, Edition 1. FAO/WHO, Codex Alimentarius Commision, Rome. 1984.
[22]	Maobe A.G., Gatebe E., Gitu l., and Rotich H., “Profile of heavy metals in selected medicinal plants used for the treatment of diabetes, malaria and pneumonia in Kisii region, Southwest Kenya”. Global Journal of Pharmacology, 6(3), 245-251, 2012.
[23]	Ullah R., Khader J.A., Hussain I.,AbdElsalam N.M., Talha M., Khan N., “Investigation of macro and micro nutrients in selected medicinal plants”. African Journal of Pharmacy and Pharmacology, 6(25), 1829-1832, 2012.
[24]	Corbett J.V., “Accidental poisoning with iron supplements”. American Journal of Maternity Child Nursing, 20, 234, 1995.
[25]	Jabeen S., Shah M.T., Khan S.,Hayat M.Q., “Determination of major and trace elements in ten important folk therapeutic plants of Haripur basin”. Pakistan Journal of Medicinal Plants Research, 4(7), 559-566, 2010.
[26]	Nkansah M.A. and Amoako C.O., “Heavy metal content of some common spices available in market in the Kumasi metropolis of Ghana”. American Journal of Scientific and Industrial Research, 1(2), 158-163, 2010.
[27]	deMan J.M Principles of food Chemistry Van Nostrand Reinhold, New York, 1990, p 429.