Development of Murine Model for Breast Cancer Metastasis to Bone

Komal Talreja¹ and Archana Moon^1,

¹University Department of Biochemistry, Rashtrasant Tukadoji Maharaj Nagpur University Nagpur

Cite this paper:
Komal Talreja and Archana Moon. Development of Murine Model for Breast Cancer Metastasis to Bone. Journal of Cancer Research and Treatment. 2017; 5(1):20-27. doi: 10.12691/jcrt-5-1-4

Abstract

Breast cancer is the second most common diagnosed cancer. 70% of breast cancers metastasize to the bone. This metastasis is more serious than the original tumor. The present study deals with the generation of animal (rat) model for breast cancer metastasis to bone by insertion of human breast cancer cell line MDA-MB 231. The cell line MDA-MB 231 was administered in the immunosupressed rat femur bone and tumor formation was observed for two months. Blood parameters viz; SGOT, SGPT, ALP, TRAP and calcium and antioxidant enzymes (catalase, SOD, GSH, GSSG, GST, MDA and GPX) in liver tissue were studied after one month and two months of insertion. In our study we observed increase in the antioxidant liver enzyme levels i.e. Catalase, SOD, GSH, GSH and MDA, and SGOT, ALP, TRAP and calcium levels also were increased in serum. Increased levels of antioxidant enzymes are indicative of increases Reactive oxygen species (oxidative stress) are involved in initiation, promotion and progression of carcinogenesis. Further, bone histopathology results indicate necrotic bone in experimental rats, which suggests osteolytic bone metastasis. This developed model for breast cancer metastasisto bone is cost effective and gives insight into the metastatic colonization, in which initially formed micrometastasis succeed in colonizing the marrow and generating osteolytic metastasis.

Keywords:
breast cancer metastasis cancer cell line tumor

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References:

[1]	Robert H Goldstein, Robert A Weinberg, and Michael Rosenblatt, Of Mice and (Wo)men: Mouse Models of Breast Cancer Metastasis to Bone, Journal of Bone and Mineral Research, Vol. 25, No. 3, March 2010, pp 431-436.
[2]	Tobias Ba¨uerle, Hassan Adwan, Fabian Kiessling, Heidegard Hilbig, Franz P. Armbruster and Martin R. Berger. Characterization of a rat model with site-specific bone metastasis induced by MDA-MB-231 breast ancer cells and its application to the effects of an antibody against bone sialoprotein., Int. J. Cancer: 115, 177-186 (2005).
[3]	Ann Rheum Dis Leflunomide: mode of action in the treatment of rheumatoid arthritis, Annals of the rheumatic diseases 2000; 59: 841-849.
[4]	Janet E. Price, Aristidis Polyzos, Ruo Dan Zhang, and Lisa M. Daniels Tumorigenicity and Metastasis of Human Breast Carcinoma Cell Lines in Nude Mice1, , [CANCER RESEARCH 50, 717-721. February) I, 1990).
[5]	D. Proschek, m.g. mack, a.a. kurth, p. Proschek, b. Martin, m.l. hansmann and t.j. vogl, Radiofrequency Ablation of Experimental Bone Metastases in Nude Rats , anticancer research 28: 879-886 (2008).
[6]	Komal talreja, archana moon*, surbhi shivhare, ninad moon, Akshay dhoble, sanjay ingle and radhika Pagey, In vivo toxicity profile of brassica oleracea l.Var.capitata (cabbage), international journal of pharma and bio sciences, int j pharm bio sci; 6(3): (p) 408-419, 2015 july.
[7]	Ic-h. William lau,’ tomoyuld onishi, jon e. Wergedal, frederick a. Singer, and david j. Bayllnk, Characterization and assay of Tartarate–Resistant Acid Phosphatase activity in serum: potential use to assess bone resorption. CLIN.CHEM.33/4, 458-462 (1987).
[8]	Mahmoud hussein hadwan*, lamia a. Almashhedy And abdul razzaq s. Alsalman, Precise method for the assessment of catalase-like Activity in seminal fluids. International journal of pharma and bio sciences Int j pharm bio sci; 4(1): (p) 949-954, 2013 jan.
[9]	Stefan MARKLUND and Gudrun MARKLUND Involvement of the Superoxide Anion Radical in the Autoxidation Of Pyrogallol and a Convenient Assay for Superoxide Dismutase Department of Chemistry, Section of Physiological Chemistry, University of Umei Eur. J. Biochem. 47,469-474 (1974).
[10]	B. E. Davidson and f. J. R. Hird The Estimation of Glutathione in Rat Tissues, A comparison of a new spectrophotometric method with the Glyoxalase method, By biochem. J. (1964), 93, 232.
[11]	Sarita chavan, laxmayya sava, vishal saxena, sandhya pillai, alka sontakke and digamber ingole.Reduced glutathione : importance of specimen collection. Indian Journal of Clinical Biochemistry, 20 (1) 150-152, 2005.
[12]	Daniela Giustarini1, Isabella Dalle-Donne2, Aldo Milzani2, Paolo Fanti3,4 & Ranieri Rossi Analysis of GSH and GSSG after derivatization with N-ethylmaleimide, , nature protocols, VOL.8 NO.9 |2013|.
[13]	Wallert and Provost Lab Enzyme Assay for Glutathione S-Transferase.
[14]	Moumita Dutta, Utpal Kumar Biswas, Runu Chakraborty, Piyasa Banerjee, Arun Kumar, Utpal Raychaudhuri Enhanced antioxidant enzyme activity in tissues and reduced total oxidative stress in plasma by the effect of Swietenia macrophylla king seeds in type II diabetes rats, International Journal of Herbal Medicine, ISSN 2321-2187 IJHM; 1 (6): 31-36, 2014.
[15]	Adekunle Adeniran Sanmi Oxidative Stress in Biochemical, Seminological and Histological Alterations Due to Acute Administration of Intramuscular Artemether in Mice, http://www.sciencepub.net/researcher.
[16]	ab150681 Picro-Sirius Red Stain Kit (Connective Tissue Stain), abcam.
[17]	Centre for Musculoskeletal Research, University of Rochester medical centre.
[18]	Sirius Red Staining Protocol for Collagen, John A. Kiernan, NovaUltra Special Stain Kits.
[19]	Nagwa A. Ismail, Sawsan H. Okasha, A. Dhawan, Azza O. Abdel-Rahman, Olfat G. Shaker, and Nehal A. Sadik. Antioxidant Enzyme Activities in Hepatic Tissue from Children with Chronic Cholestatic Liver Disease.
[20]	Sarah O. Nwozo1, Owumi Solomon, Oresajo Oluwakemi Abimbola and Dada Omolayo. Kikelomo1Comparative Study of Biochemical and Nutritional Status of Breast Cancer Patients on Chemotherapy/Radiotherapy in Ibadan American Journals of Cancer Science http://ivyunion.org/index.php/ajcs/ Vol. 1, Article ID 20130091, 10 pages.
[21]	Tapasi Rana, Anwesa Chakrabarti, Michael Freeman, Swati Biswas. Doxorubicin-Mediated Bone Loss in Breast Cancer Bone Metastases Is Driven by an Interplay between Oxidative Stress and Induction of TGFb PLOS ONE | www.plosone.org 1 | Volume 8 | Issue 10 | e78043, October 2013.