Ameliorative Potential of Different Doses of Indol-3-carbinol on Doxorubicin-induced Cardiotoxicity in Mice

Almokhtar A. Adwas¹, Abeer A. Elkhoely², Ahmed M. Kabel^3, , Mohamed Nabih Abdel-Rahman³ and Amany A. Eissa²

¹Pharmacology department, Faculty of Medicine, Zawia University, Lybia

²Pharmacology and toxicology department, Faculty of Pharmacy, Helwan University, Egypt

³Pharmacology department, Faculty of Medicine, Tanta University, Egypt

Cite this paper:
Almokhtar A. Adwas, Abeer A. Elkhoely, Ahmed M. Kabel, Mohamed Nabih Abdel-Rahman and Amany A. Eissa. Ameliorative Potential of Different Doses of Indol-3-carbinol on Doxorubicin-induced Cardiotoxicity in Mice. Journal of Cancer Research and Treatment. 2016; 4(2):26-31. doi: 10.12691/jcrt-4-2-2

Abstract

Background: Doxorubicin (DOX) is a commonly used chemotherapeutic agent that is associated with serious dose-limiting cardiotoxicity. This cardiotoxicity was attributed to various mechanisms including induction of oxidative stress and inflammation together with inhibition of apoptosis. Indole-3-carbinol (I3C) is a phytochemical that was suggested to have potent anti-oxidant and anti-inflammatory properties. Aim: It was to detect the possible ameliorative effects of different doses of I3C on doxorubicin-induced cardiotoxicity in mice. Methods: Eighty mice were divided into four equal groups: control untreated group; DOX group; DOX + I3C 1000 ppm group and DOX + I3C 2000 ppm group. Survival rate, serum creatine kinase (CK-MB), lactate dehydrogenase (LDH) and troponin I were measured. Also, tissue malondialdehyde (MDA), tissue catalase (CAT), tissue glutathione peroxidase (GPx), and tissue tumor necrosis factor alpha (TNF-α) were determined. Parts of the heart were subjected to histopathological examination. Results: I3C produced dose-dependent significant increase in the survival rate, tissue GPx and CAT with significant decrease in serum CK-MB, LDH, troponin I, tissue MDA and TNF-α and improved the histopathological and immunohistochemical changes compared to DOX-treated group. Conclusion: I3C- in a dose dependent manner- had a protective effect against doxorubicin-induced cardiotoxicity in mice.

Keywords:
indole-3-carbinol doxorubicin cardiotoxicity mice

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