Histone H1.5 Expression in Prostatic Carcinoma: An Immunohistochemical Study

Mohamed A. El-Rashidy¹, Asmaa E. Bedeer¹ and Ahmed M. Kabel^{2, 3,}

¹Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

²Pharmacology department, Faculty of Medicine, Tanta University, Tanta, Egypt

³Department of clinical pharmacy, College of Pharmacy, Taif University, Taif, Saudi Arabia

Cite this paper:
Mohamed A. El-Rashidy, Asmaa E. Bedeer and Ahmed M. Kabel. Histone H1.5 Expression in Prostatic Carcinoma: An Immunohistochemical Study. Journal of Cancer Research and Treatment. 2016; 4(2):21-25. doi: 10.12691/jcrt-4-2-1

Abstract

Background and Aim: Histone H1.5 (HH1.5) is a subtype of histone H1, a family of linker proteins that is known to determine chromatin structure, alter gene expression and DNA repair. It also contributes to regulation of cell proliferation in breast cancer. In this study, we aimed to investigate the immunohistochemical expression of HH1.5 in various prostatic lesions. Methods: A total 50 cases of various prostatic biopsies were studied. Histone H1.5 expression was evaluated in all cases. HH1.5 expression was scored as negative (<11%), 1+ (11-50%), or 2+ (>50%). Correlations between the intensity and differential localization of these markers and Gleason patterns were evaluated. Results: HH1.5 immunohistochemistry revealed positive nuclear reactivity in all cases (100%) of prostate adenocarcinomas, compared to only 2 (11%) of 18 cases of benign prostatic glands (P ≤ 0.001). In all positive benign prostate epithelium, HH1.5 was limited to focal and weak reactivity. Similarly, both the two cases of high-grade prostatic intraepithelial neoplasia exhibited focal weak nuclear reactivity. Increased HH1.5 reactivity was observed in Gleason patterns 5 and 4 as compared to Gleason pattern 3, 100%, 64.7% and 50%, respectively (P ≤ 0.002). Conclusion: HH1.5 may be a useful diagnostic tool in evaluating prostatic biopsies, particularly with small foci of cancer. Further studies are needed to support these findings and investigate the possible prognostic significance of HH1.5 in prostatic adenocarcinomas.

Keywords:
histone expression prostate carcinoma

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