Study of p53, Pcna, Ki67 and Micronuclei Related to Genotoxic Damage for Risk Categorization in Pre-Malignant Oral Lesions

Asoke Roy^1, , Satyendra Prakash Bhatnagar², Malay Chatterjee³, Dipanwita Ghosh¹, Goutam Mandal⁴, Shyamsundar Mondal⁵ and Shyamal Goswami⁶

¹Department of Pathology and Cervical Screening, Chittaranjan National Cancer Institute, Kolkata, India

²Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, India

³Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India

⁴Department of Pathology, Hospital wing, Chittaranjan National Cancer Institute, Kolkata, India

⁵Department of Statistics and Records, Chittaranjan National Cancer Institute, Kolkata, India

⁶Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India

Cite this paper:
Asoke Roy, Satyendra Prakash Bhatnagar, Malay Chatterjee, Dipanwita Ghosh, Goutam Mandal, Shyamsundar Mondal and Shyamal Goswami. Study of p53, Pcna, Ki67 and Micronuclei Related to Genotoxic Damage for Risk Categorization in Pre-Malignant Oral Lesions. Journal of Cancer Research and Treatment. 2014; 2(1):10-15. doi: 10.12691/jcrt-2-1-3

Abstract

In this prospective study, search for high risk cases of premalignant oral lesions were attempted through evaluation of oncoprotein expression, cell proliferation and micronuclei. Materials and methods: A total 50 cases of oral leukoplakia were diagnosed and adequate controls along with detailed history were included in this study. Study of tumour markers like p53, PCNA and Ki-67 were done immunohistochemically on tissue sections. Study of micronuclei was performed by feulgen staining method. Results: The mean age of the 50 cases were 44.48 ± 9.76 and median age was 50 years. There were 45 male cases (90%) and 5 female cases (10%). There were 26 non dysplastic (52%) and 24 dysplastic cases (48%). The smokers consist of 25 cases (50%) and other addictions (betel quid chewers, ghutka, khaini, alcohol etc.) had 24 cases (48%) and 1 case was non addict (2%). Out of 25 cases of smokers, 20 (80%) were positive in case of p53, 19 (76%) were positive in case of PCNA and 17 (68%) were positive in case of Ki67. Out of 24 cases of other addictions, 16 (66.67%) cases were positive in case of p53, 13 (54.17%) positive cases in case of PCNA and16 cases (66.67%) were positive in case of Ki67.The smokers had 0.51 ± 0.21 micronuclei frequency whereas other addiction groups had 0.24 ± 0.10. Conclusion: The histopathological risk assessment of these cases is inconclusive and thus p53, PCNA, Ki67 and micronuclei evaluation are needed for risk categorization.

Keywords:
oral leukoplakia oral squamous cell carcinoma immunohistochemistry molecular-markers p53 PCNA Ki67 micronuclei epithelial dysplasia

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