The Daily Light-dark Cycle of Photosynthetic Oxygen Evolution in Three Species of Tropical Calcareous Algae

Alvin P. Monotilla^1, , Serafin M. Geson¹, Paul Isaac O. Dizon¹ and Danilo T. Dy¹

¹Marine Biology Section, University of San Carlos, Cebu City, Philippines

Cite this paper:
Alvin P. Monotilla, Serafin M. Geson, Paul Isaac O. Dizon and Danilo T. Dy. The Daily Light-dark Cycle of Photosynthetic Oxygen Evolution in Three Species of Tropical Calcareous Algae. Journal of Aquatic Science. 2015; 3(1):14-18. doi: 10.12691/jas-3-1-3

Abstract

Endogenous coordination between light, temperature and other factors by different species of algae would be vital in the production of several proteins needed for growth and adaptations, and therefore will affect their productivity. Among plant activities that are governed by daily light-dark cycles (i.e. 12L:12D and 12L:12L) among calcareous algae (Halimeda simulans, Mastophora rosea and Padina australis) were conducted by monitoring the dissolved oxygen (DO) concentration inside incubating bottles with algal samples for 24h and the features of the resulting DO curves were determined by measuring the number of pixel under the curve through image analysis. There was no significant difference among algae during the initial 12L:12D cycle suggesting their normal response to light-dark cycles. However, after six days under continuous light, M. rosea showed a significant decrease in the DO curve (lesser number of pixel under the curve) compared to DO curves during the initial 12L:12D cycle. The decrease in the DO concentration during the continuous L:L treatments might be attributed to the photoinhibitory effect of the red alga being less adoptive to subsequent high intensities. Although an increase in DO concentrations is expected with continuous light, not all algae responded to it. Only Padina exhibited circadian rhythm in our 24h observation under continuous light.

Keywords:
Coral reef Padina Halimeda Mastophora circadian rhythm

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References:

[1]	Aguilera J, Figueroa FL, Häder DP and Jiménez C. 2008. Photoinhibition and photosynthetic pigment reorganisation dynamics in light/darkness cycles as photoprotective mechanisms of Porphyraumbilicalis against damaging effects of UV radiation. Scientia Mar. 72(1): 87-97.
[2]	Ahmad, M., Grancher, N., Heil, M., Black, R. C., Giovani, B., Galland, P. and Lardemer, D. 1999. Action Spectrum for Cryptochrome-Dependent Hypocotyl Growth Inhibition in Arabidopsis. Plant Physiol. 129: 774-785.
[3]	Argyroudi-Akoyunoglou JH and Pombana A. 1996. Light-independent endogenous circadian rhythm in the capacity for chlorophyll formation. J. Photochem. Photobiol. 36:271-277.
[4]	Bischoff K, Gomez I, Molis M, Hanelt D, Karsten U, Lüder U, Roleda MY, Zacher K andWiencke C. 2006. Ultraviolet radiation shape seaweeds. Rev. Environ. Sci. Biotechnol. 26 pp.
[5]	Chow F, de Oliveira MC and Pedersén M. 2004. In vitro assay and light regulation of nitratereductase in red alga Gracilariachilensis. J. Plant Physiol. 161: 769-776.
[6]	Cunningham FX1, Dennenberg RJ, Mustardy L, Jursinic PA, Gantt E. 1989. Stoichiometry of Photosystem I, Photosystem II, and Phycobilisomes in the Red Alga Porphyridiumcruentum as a Function of Growth Irradiance. Plant Physiol. 91(3):1179-87.
[7]	Drew EA and Abel KM. 1990.Studies on Halimeda III. A Daily Cycle of Chloroplast Migration within segments. Botanica Marina 33: 31-45.
[8]	Drew EA and Abel KM. 1992. Studies on Halimeda IV.An endogenous rhythm of chloroplastmigration in the siphonous green alga, H. distorta. J. Interdisciplinary Cycle Res. 23 (2): 128-236.
[9]	Drew EA and Abel KM. 1995. Studies on Halimeda V. Effect of temperature on chloroplastmigration in this siphonous green alga. Biol. Rhythm Res. 26 (1): 48-54.
[10]	Granbom M, Pedersén M, Kadel P and Lüning K. 2001. Circadian rhythm of photosyntheticoxygen evolution in Kappaphycusalvarezii (Rhodophyta): dependence on light quantity and quality. J. Phycol. 37:1020-1025.
[11]	Koop H U, Schmid R, Heunert H H and Milthaler B. 1978. Chloroplast Migration: a New Circadian Rhythm in Acetabularia. Protoplasma 97: 301-310.
[12]	Larkum AWD, Salih A, Kühl M. 2011.Rapid mass movement of chloroplasts during segmentformation of the calcifying siphonalean green alga, Halimedamacroloba. PLoS ONE 6(7): e20841.
[13]	Lesser M P. 1996. Elevated temperatures and ultraviolet radiation cause oxidative stress and inhibit photosynthesis in symbiotic dinoflagellates. Limnol. Oceanogr. 41(2): 271-283.
[14]	Lopes PF, de Oliveira MC and Colepicolo P. 2002. Characterization and daily variation of nitratereductase in Gracilariatenuistipitata (Rhodophyta). Biochem. Biophys. Res. Comm. 295:50-54.
[15]	Mishkind M, Mauzerall D and Beale SI. 1979. Diurnal variation in situ of photosynthetic capacity in Ulva is caused by a dark reaction. Plant Physiol. 64:896-899.
[16]	Osmond C B, Ramus J, Levavasseur, G, Franklin, L A, Henley W H. 1993. Fluorescence quenching during photosynthesis and photoinhibition of Ulvarotundata blid. Planta 190(1): 97-106.
[17]	Sagert S, Forster RM, Feuerpfeil P and Schubert H.1997. Daily course of photosynthesis andphotoinhibition in Chondruscrispus (Rhodophyta) from different shore levels. European J. of Phycol 32(4): 363-371.
[18]	Van Alstyne K, McCarthy J III, Hustead CL and Kearns LJ. 1999. Phlorotannin allocation among tissues of Northeastern Pacific kelps and rockweeds. J. Phycol. 35: 483-492.
[19]	Yen, U. C., Huang, T. C. and Yen, T. C. 2004.Observation of the circadian photosynthetic rhythm in cyanobacteria with a dissolved-oxygen meter. Pl. Sci. 166: 949-952.