Laser Biotechnology for Enhanced Rooting and Shooting of Salix Viminalis in Hydroponic Condition for Better Adaptation in Industrially Contaminated Land

Bigyan Rimal^1, , Rindra Manasoa Ranaivoson¹, Katarzyna Podlejska Czarnecka¹ and Jan W. Dobrowolski¹

¹AGH University of Science and Technology, UNESCO AGH Chair, Krakow, Poland

Cite this paper:
Bigyan Rimal, Rindra Manasoa Ranaivoson, Katarzyna Podlejska Czarnecka and Jan W. Dobrowolski. Laser Biotechnology for Enhanced Rooting and Shooting of Salix Viminalis in Hydroponic Condition for Better Adaptation in Industrially Contaminated Land. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(5):228-230. doi: 10.12691/ijebb-2-5-3

Abstract

Laser Photo stimulation of selected plant and microbial species has been very important in reclamation of several degraded land masses as well as effective treatment of waste and contaminated water sources. The experiment on Laser Photo-stimulation of different size of willow cuttings is carried out in laboratory condition at Department of Environment protection Engineering of AGH-UST, Krakow, Poland. Laser irradiation with selected wavelength and time of exposition of willow cuttings and establishment in hydroponic culture system resulted in enhanced development of roots and shoots compared to the non-irradiated control group. Experimental observation made every week after the irradiation has results of increased number of roots and sprouts. Root buds also increased rapidly in number after second week. Roots changed into highly dispersed secondary roots in II and IV experimental groups and sprouts also started to develop leaves after second week. Remarkable differences in control and experimental groups were seen after five weeks of hydroponic culture. The enhanced development of roots, shoots and other protective organs like root caps after laser irradiation is supposed to help willow species to grow fast and adapt in industrially contaminated land.

Keywords:
laser biostimulation willow root shoot cuttings

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References:

[1]	Dobrowolski et. al, 2012, Laser Biotechnology for more efficient bioremediation, protection of aquatic ecosystems and reclamation of contaminated areas; Wiley Online Library.
[2]	Dobrowolski J.: New perspectives of the application of laser biotechnology to improve water quality. NEAR International Symposium on the Assessment Disposal and Treatment of Rural Wastes. The Protection of Freshwater Resources, Rivers, Lakes and Groundwater, Politechnika Krakowska, 20-22, 1998.
[3]	Dobrowolski et. al, 2012, Laser Biotechnology for more efficient bioremediation, protection of aquatic ecosystems and reclamation of contaminated areas; Wiley Online Library.
[4]	Dobrowolski et. al, 2012, Laser stimulation of Trichophyton mentagrophytes for the enhancement biodegradation of hydrocarbons; Environmental Engineering and Management Journal; Vol. 11, No. 10, 1783-1788.
[5]	Hanna Obarska-Pempkowiak, Katarzyna Kołecka: Experiences of Salix viminalis application to water and sewage treatment, Department of Water and Waste-Water Technology, Faculty Civil and Environmental Engineering, Gdańsk University of Technology, Narutowicza 11/12, 80-952 Gdańsk.
[6]	Karu TI, Pyatibrat L, Kalendo G (1995) Irradiation with He-Ne laser increases ATP level in cells cultivatedin vitro.J Photochem Photobiol B27: 219-23.
[7]	Oron U, Yaakobi T, Oron A, Mordechovitz D, Shofti R, Hayam Get al.(2001) Low-energy laser irradiation reduces formation of scar tissue after myocardial infarction in rats and dogs. Circulation 103: 296-301.
[8]	Sommer AP, Pinheiro AL, Mester AR, Franke RP, Whelan HT (2001) Biostimulatory windows in low intensity laser activation: lasers, scanners and NASA’s light-emitting diode array system.J Clin Laser Med Surg 19: 29-34.
[9]	Yu W, Naim JO, Lanzafame RJ (1994) The effects of photo-irradiation on the secretion of TGF and PDGF from fibroblasts in vitro. Lasers Surg Med suppl 6: 8.
[10]	Letokhov V (1983) Laser-induced chemistry. Nature 305: 103-8.
[11]	Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM et al.(1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium-neon laser. FEBS Lett 175: 95-9.
[12]	Pastore D, Greco M, Passarella S (2000) Specific helium-neon laser sensitivity of the purified cytochromecoxidase.Int J Radiat Biol 76: 863-70.
[13]	Salet C, Passarella S, Quagliariello E (1987) Effects of selective irradiation on mammalian mitochondria. Photochem and Photobiol 45: 433-8.
[14]	Belkin M, Zaturunsky B, Schwartz M (1988) A critical review of low energy laser bioeffects. Lasers Light Ophthalmol 2: 63-71.
[15]	Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation. J Clin Periodontol 23: 492-6.
[16]	Wan-Ping Hu, Jeh-Jeng Wang, Chia-Li Yu, Cheng-Che E Lan, Gow-Shing Chen and Hsin-Su Yu (2007) Journal of Investigative Dermatology (2007) 127, 2048-2057; Helium-Neon Laser Irradiation Stimulates Cell Proliferation through Photostimulatory Effects in Mitochondria.