Exploring the Strength of Pseudomonas –a & Pseudomonas B in Removal of ClO₄^- & ClO₃^-: an Outstanding Approach of Environmental Bioremediation

Maulin P Shah^1,

¹Industrial Waste Water Research Laboratory Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP)

Cite this paper:
Maulin P Shah. Exploring the Strength of Pseudomonas –a & Pseudomonas B in Removal of ClO₄^- & ClO₃^-: an Outstanding Approach of Environmental Bioremediation. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(2):55-61. doi: 10.12691/ijebb-2-2-2

Abstract

Elimination of perchlorate (ClO₄^-) and chlorate (ClO₃^-) can be viewed as a very promising water treatment technology. The process is based on the ability of specific bacteria to use (per) chlorate as an electron acceptor in the absence of oxygen. The present research work was focused on the isolation and kinetic characterization of perchlorate reducing bacteria. The enrichment process started with a sludge sample taken from an anaerobic digester of a domestic wastewater treatment plant (Ankleshwar, India). Two perchlorate-reducing bacteria (A) and (B) were isolated using different selection methods, platting and liquid transfer respectively. The purity of the isolates was confirmed by genetic characterization of 16S rDNA. The BLAST search showed that the microorganims shared a 99% sequence similarities to the 16S rDNA of Pseudomonas sp. (A) and Pseudomonas sp. (B). Batch tests were performed under anaerobic conditions with acetate as the electron donor and perchlorate and/or chlorate as electron acceptor. During perchlorate reduction by Pseudomonas sp. (A) it was observed transient accumulation of chlorate. The isolates showed different behaviour concerning perchlorate and chlorate reduction. Chlorate was preferentially reduced when both electron acceptors were present, being perchlorate reduced after completely depletion of chlorate. The former performance was observed in both bacteria.

Keywords:
perchlorate and chlorate reduction kinetic characterization pseudomonas

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